Invitrogen

KIF3C Polyclonal Antibody

查看其他3个KIF3C抗体

问题与答案

问题与答案

问题与答案

抗体检测数据 (3)

KIF3C Antibody in Immunocytochemistry (ICC/IF)

KIF3C Antibody in Immunocytochemistry (ICC/IF)

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KIF3C Antibody (PA5-97899) in ICC/IF

Immunofluorescent analysis of KIF3C in A549 cells using a KIF3C polyclonal antibody (Product # PA5-97899) at a dilution of 1:200. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated Goat Anti-Rabbit IgG(H+L). Cells were counter-stained with DAPI.

KIF3C Antibody in Immunocytochemistry (ICC/IF)

KIF3C Antibody in Immunohistochemistry (Paraffin) (IHC (P))

KIF3C Antibody in Western Blot (WB)

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KIF3C Antibody in Immunocytochemistry (ICC/IF) — Immunofluorescent analysis of KIF3C in A549 cells using a KIF3C polyclonal antibody (Product # PA5-97899) at a dilution of 1:200. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated Goat Anti-Rabbit IgG(H+L). Cells were counter-stained with DAPI.

KIF3C Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemical analysis of KIF3C in paraffin embedded human glioma using a KIF3C polyclonal antibody (Product # PA5-97899) at a dilution of 1:600. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.

KIF3C Antibody in Western Blot (WB) — Western Blot analysis of KIF3C using a KIF3C Polyclonal antibody (Product # PA5-97899) at a concentration of 4.2 µg/mL. Positive WB detected in: Hela whole cell lysate, K562 whole cell lysate. A secondary Goat polyclonal antibody to rabbit IgG was applied at a 1:50,000 dilution. Observed band size: 90 kDa.

Immunogen image

KIF3C Polyclonal Antibody

Immunogen Image KIF3C Polyclonal Antibody

产品信息

PA5-97899

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1:500-1:5,000

-

免疫组化（石蜡） (IHC (P))

1:500-1:1,000

-

免疫细胞化学 (ICC/IF)

1:200-1:500

-

酶联免疫吸附实验 (ELISA)

Assay-Dependent

-

产品规格

种属反应

Human

宿主/亚型

Rabbit / IgG

分类

Polyclonal

类型

Antibody

抗原

Recombinant Human Kinesin-like protein KIF3C protein (158-267AA)

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偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

1 mg/mL

纯化类型

Protein G

保存液

PBS, pH 7.4, with 50% glycerol

内含物

0.03% ProClin 300

保存条件

-20°C or -80°C if preferred

运输条件

Ambient (domestic); Wet ice (international)

靶标信息

KIF3C is a microtubule-based anterograde translocator for membranous organelles.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

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引用该产品

生物信息学

蛋白别名: KIF3C variant protein; Kinesin-like protein KIF3C

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基因别名: KIF3C

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UniProt ID: (Human) O14782

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Entrez Gene ID: (Human) 3797

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motor activity microtubule motor activity ATP binding microtubule binding ATPase activity microtubule binding motor protein

retrograde vesicle-mediated transport, Golgi to ER microtubule-based movement antigen processing and presentation of exogenous peptide antigen via MHC class II organelle transport along microtubule