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          Disclaimer

          Clicking the images or links will redirect you to a website hosted by BenchSci that provides third-party scientific content. Neither the content nor the BenchSci technology and processes for selection have been evaluated by us; we are providing them as-is and without warranty of any kind, including for use or application of the Thermo Fisher Scientific products presented.

          • Primary Antibodies ›
          • KLRG1 Antibodies

          Invitrogen

          KLRG1 Monoclonal Antibody (2F1), Alexa Fluor™ 647, eBioscience™

          9 Published Figures
          9 References
          View all (43) KLRG1 antibodies
          Datasheet
          Protocols
          Tech Support

          Cite KLRG1 Monoclonal Antibody (2F1), Alexa Fluor™ 647, eBioscience™

          Datasheet
          Protocols
          Tech Support
          • Antibody Testing Data (1)
          • Published Figures (9)
          KLRG1 Antibody in Flow Cytometry (Flow)
          Group 53 Created with Sketch.

          FIGURE: 1 / 10

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          KLRG1 Antibody (51-5893-82) in Flow

          C57BL/6 mouse splenocytes were stained with NK1.1 Monoclonal Antibody, FITC (Product # 11-5941-82) and 0.125 µg of KLRG1 Monoclonal Antibody, Alexa Fluor 647. Total viable cells were used for analysis, as determined by 7-AAD (Product # 00-6993-50). {{ $ctrl.currentElement.advancedVerification.fullName }} validation info. View more
          Published figure supplied by benchsci-logo
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          Product Details

          51-5893-82

          Applications
          Tested Dilution
          Publications

          流式细胞分析 (Flow)

          0.25 µg/test
          查看9篇已发表文章 9篇已发表文章
          产品规格

          Species Reactivity

          Mouse

          Published species

          Mouse

          Host/Isotype

          Syrian hamster / IgG

          分类

          Monoclonal

          类型

          Antibody

          克隆号

          2F1

          Conjugate

          Alexa Fluor™ 647
          • Alexa Fluor 488 
          • Alexa Fluor 532 
          • Alexa Fluor 561
          • APC 
          • APC-eFluor 780
          • Biotin 
          • eFluor 450
          • eFluor 506
          • FITC
          • Functional Grade
          • Pacific Orange
          • PE 
          • PE-Cyanine5
          • PE-Cyanine5.5
          • PE-Cyanine7
          • PE-eFluor 610
          • PerCP-eFluor 710
          • Super Bright 436
          • Super Bright 600
          • Super Bright 645
          • Super Bright 702
          • Super Bright 780

          Excitation/Emission Max

          650/671 nm View spectra spectra

          形式

          Liquid

          浓度

          0.2 mg/mL

          纯化类型

          Affinity chromatography

          保存液

          PBS, pH 7.2

          内含物

          0.09% sodium azide

          保存条件

          4° C, store in dark, DO NOT FREEZE!

          RRID

          AB_2744744

          Product Specific Information

          Description: This 2F1 monoclonal antibody reacts with the mouse Killer cell Lectin-like Receptor G1 (KLRG1), also known as Mast cell Function-associated Antigen (MAFA). KLRG1 is a homodimer of glycosylated 30-38 kDa subunits and contains a cytoplasmic motif similar to the immunoreceptor tyrosine-based inhibitory motif (ITIM). Rat MAFA was identified as an antigen specific to rat mast cells; however, the expression of mouse KLRG1/MAFA using 2F1 has not been detected on the surface of mouse mast cell lines, bone marrow-derived mast cells, or peritoneal mast cells. This antigen is expressed on approximately one-third of mouse NK cells and a subset of T cells. MHC class I molecules regulate KLRG1 via interactions with class I-specific inhibitory Ly49 molecules and SHP-1 signaling. Although KLRG1 and Ly49 are both lectin-like inhibitory receptors that are regulated by class I MHC expression, the effects of this on cell surface expression of these molecules are opposing, and the underlying regulatory mechanisms distinct.

          Applications Reported: This 2F1 antibody has been reported for use in flow cytometric analysis.

          Applications Tested: This 2F1 antibody has been tested by flow cytometric analysis of mouse splenocytes. This may be used at less than or equal to 0.25 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

          Excitation: 633-647 nm; Emission: 668 nm; Laser: Red Laser.

          Target Information

          KLRG1 (Killer cell lectin-like receptor G1, MAFA, 2F1-Ag) is a homodimeric member of the lectin-like type 2 transmembrane receptor family whose members contain characteristic immunoreceptor tyrosine-based inhibitory motifs (ITIMs) in their cytoplasmic domains. These ITIMs interact with the SH2 domains of protein phosphatases such as SHP-1. KLRG1 is an inhibitory receptor that is expressed on natural killer (NK) cells and certain T cells. NK cells are involved in the lysis of tumor cells and virus-infected cells, and mediate humoral and cell-mediated immune responses.

          仅用于科研。不用于诊断过程。未经明确授权不得转售。

          Flow Cytometry
          Panel Builder

          How to use the Panel Builder

          Watch the video to learn how to use the Invitrogen Flow Cytometry Panel Builder to build your next flow cytometry panel in 5 easy steps.

          Bioinformatics

          Protein Aliases: Killer cell lectin-like receptor subfamily G member 1; Mast cell function-associated antigen 2F1; natural killer cell receptor

          View more View less

          Gene Aliases: 2F1-Ag; Klrg1; MAFA; MAFA-L

          View more View less

          UniProt ID: (Mouse) O88713

          View more View less

          Entrez Gene ID: (Mouse) 50928

          View more View less

          Molecular Function: transmembrane signal receptor

          Function(s)
          immune system process cell surface receptor signaling pathway innate immune response
          Process(es)
          carbohydrate binding
          It has to be done as per old AB suggested Products section.
          guarantee_icon

          Performance Guarantee

          If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

          Learn more
          help_icon

          We're here to help

          Get expert recommendations for common problems or connect directly with an on staff expert for technical assistance related to applications, equipment and general product use.

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