Invitrogen

Kinesin 5A Polyclonal Antibody

Kinesin 5A Antibody in Immunocytochemistry (ICC/IF)

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Kinesin 5A Antibody (PA1-642) in ICC/IF

Immunofluorescence analysis of Kinesin 5A was done on 70% confluent log phase SH-SY5Y cells. The cells were fixed with 4% paraformaldehyde for 15 minutes, permeabilized with 0.25% Triton™ X-100 for 10 minutes, and blocked with 5% BSA for 1 hour at room temperature. The cells were labeled with Kinesin 5A Rabbit Polyclonal Antibody (Product # PA1-642) at 1 µg/mL in 1% BSA and incubated for 3 hours at room tem... View More

Kinesin 5A Antibody in Western Blot (WB)

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产品信息

PA1-642

应用

建议稀释比

已发表文章

免疫印迹 (WB)

0.5-2 µg/mL

查看4篇已发表文章 4篇已发表文章

免疫细胞化学 (ICC/IF)

1-2 µg/mL

免疫沉淀 (IP)

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产品规格

种属反应

Bovine, Human, Mouse

已发表种属

Human, Mouse

宿主/亚型

Rabbit / IgG

分类

Polyclonal

类型

Antibody

抗原

Synthetic Peptide: C(1007) G Y E A E D Q A K L F P L H Q E T A A S(1027)

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偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

1 mg/mL

纯化类型

Antigen affinity chromatography

保存液

PBS with 1mg/mL BSA

内含物

0.05% sodium azide

保存条件

-20°C, Avoid Freeze/Thaw Cycles

运输条件

Ambient (domestic); Wet ice (international)

AB_2132216

产品详细信息

PA1-642 detects kinesin 5A from mouse, human, and bovine samples. This antibody is specific for kinesin 5A and does not detect other kinesin isotypes.

PA1-642 has been successfully used in Western blot procedures. By Western blot, this antibody detects an ~110 kDa protein representing kinesin 5A from mouse neuronal cell lysate and human retinal extracts.

The PA1-642 immunizing peptide corresponds to amino acid residues 1007-1027 from mouse kinesin 5A. This sequence is completely conserved in human. This peptide (Cat. # PEP-197) is available for use in neutralization and control experiments.

靶标信息

This gene encodes a member of the kinesin family of proteins. Members of this family are part of a multisubunit complex that functions as a microtubule motor in intracellular organelle transport. Mutations in this gene cause autosomal dominant spastic paraplegia 10.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

Product References

Truncated Tau Induces Mitochondrial Transport Failure Through the Impairment of TRAK2 Protein and Bioenergetics Decline in Neuronal Cells.

Frontiers in cellular neuroscience

Quintanilla RA,Tapia-Monsalves C,Vergara EH,Pérez MJ,Aranguiz A

PA1-642 was used in Western Blotting to study the possible role of motor proteins (kinesin 1 and dynein) and mitochondrial protein adaptors (RhoT1/T2, syntaphilin, and TRAK2) in the mitochondrial transport failure induced by caspase-cleaved tau.

Mon Sep 28 00:00:00 UTC 2020

Truncated Tau Induces Mitochondrial Transport Failure Through the Impairment of TRAK2 Protein and Bioenergetics Decline in Neuronal Cells.

Frontiers in cellular neuroscience

Quintanilla RA,Tapia-Monsalves C,Vergara EH,Pérez MJ,Aranguiz A

Mon Sep 28 00:00:00 UTC 2020

Disparate Regulatory Mechanisms Control Fat3 and P75NTR Protein Transport through a Conserved Kif5-Interaction Domain.

PloS one

Cheng H,Burroughs-Garcia J,Birkness JE,Trinidad JC,Deans MR

PA1642 was used in immunoprecipitation and western blot to elucidate the regulation of Fat3 localization

Tue Jun 06 00:00:00 UTC 2017

Neurochemical profile of dementia pugilistica.

Journal of neurotrauma

Kokjohn TA,Maarouf CL,Daugs ID,Hunter JM,Whiteside CM,Malek-Ahmadi M,Rodriguez E,Kalback W,Jacobson SA,Sabbagh MN,Beach TG,Roher AE

PA1-642 was used in western blot to study the biochemical/molecular changes associated with dementia pugilistica

Sat Jun 01 00:00:00 UTC 2013

APP anterograde transport requires Rab3A GTPase activity for assembly of the transport vesicle.

The Journal of neuroscience : the official journal of the Society for Neuroscience

Szodorai A,Kuan YH,Hunzelmann S,Engel U,Sakane A,Sasaki T,Takai Y,Kirsch J,Müller U,Beyreuther K,Brady S,Morfini G,Kins S

PA1-642 was used in western blot to investigate the role of the Rab3A GTPase during the APP anterograde transport.

Wed Nov 18 00:00:00 UTC 2009

Conventional kinesin holoenzymes are composed of heavy and light chain homodimers.

Biochemistry

DeBoer SR,You Y,Szodorai A,Kaminska A,Pigino G,Nwabuisi E,Wang B,Estrada-Hernandez T,Kins S,Brady ST,Morfini G

PA1-642 was used in western blot to investigate the composition of the kinesin holoenzymes

Tue Apr 15 00:00:00 UTC 2008

生物信息学

蛋白别名: KIF5A variant protein; Kinesin heavy chain isoform 5A; Kinesin heavy chain neuron-specific 1; kinesin, heavy chain, neuron-specific; Neuronal kinesin heavy chain; NKHC

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基因别名: D10Bwg0738e; D12S1889; Khc; Kiaa4086; Kif5; KIF5A; Kns; mKIAA4086; MY050; NKHC; NKHC1; SPG10

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UniProt ID: (Human) Q12840, (Mouse) P33175

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Entrez Gene ID: (Human) 3798, (Bovine) 511273, (Mouse) 16572

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功能

motor activity protein binding ATP binding microtubule binding ATP-dependent microtubule motor activity, plus-end-directed microtubule motor activity protein complex binding microtubule binding motor protein

参与通路

retrograde vesicle-mediated transport, Golgi to ER microtubule-based movement chemical synaptic transmission axon guidance protein localization antigen processing and presentation of exogenous peptide antigen via MHC class II cytoskeleton-dependent intracellular transport