Identifyn

Ku70 Polyclonal Antibody, Identifyn™

Name: Ku70 Polyclonal Antibody, Identifyn\u2122
Brand: Identifyn
SKU: PA-RP-000006

查看其他33个Ku70抗体

说明书

实验方案

问题与答案

技术支持

说明书

实验方案

问题与答案

技术支持

说明书

实验方案

问题与答案

技术支持

抗体检测数据 (9)

Ku70 Antibody in Immunocytochemistry (ICC/IF)

图: 1 / 9

Ku70 Antibody (PA-RP-000006) in ICC/IF

Identifyn Detailed protocols can be found below under Documents & Downloads. Control Image: Plus Damage-Panel A. HeLa cells were grown on acid-etched 1.5H cover glass to ~50% confluency. DNA damage was induced via treatment with 2 µM Etoposide-spiked complete growth media for 24 hours, followed by -20°C methanol fixation and blocked with Thermo Fisher Fish Serum Blocker (Product # 37527X3). The primary antibody, Rabbit anti-Human Ku70 Polyclonal, IgG, was incubated for 1 hour at room temperature at a dilution of 1:250. Cells were washed 3X... View More

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Immunogen image

Immunogen Image Ku70 Polyclonal Antibody, Identifyn™

产品信息

PA-RP-000006

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1:250-1:1,000

免疫细胞化学 (ICC/IF)

1:250-1,000

产品规格

种属反应

Human

宿主/亚型

Rabbit / IgG

分类

Polyclonal

类型

Antibody

抗原

Peptide Sequences between Residues 205-256 and 573-623, referencing both Uniprot P12956 and Gene ID 2547.

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偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

1 mg/mL

纯化类型

Affinity chromatography

保存液

PBS, pH 7.4, with 10% glycerol

内含物

0.02% sodium azide

保存条件

4°C

运输条件

Ambient (domestic); Wet ice (international)

产品详细信息

Identifyn™ Primary and Secondary antibodies are extensively validated in Immunofluorescence assays by traditional as well as high resolution acquisition like Widefield SIM, Confocal, Airyscan and STORM Microscopy.

Recommended dilution for traditional microscopy:

Widefield - 1:250-1:1,000

Widefield SIM - 1:250-1:1,000

Confocal - 1:250-1:1,000

Recommended dilution for Super Resolution microscopy:

SIM (Structured Illumination Microscopy) - 1:1,000

Single Molecule STORM (Stochastic Optical Reconstruction Microscopy) - 1:100-1:1,000

Detailed protocols can be found below under Documents & Downloads.

靶标信息

XRCC6 is a nuclear complex consisting of two subunits with molecular masses of approximately 70 and 80 kDa. The complex functions as a single-stranded DNA-dependent ATP-dependent helicase. The complex may be involved in the repair of nonhomologous DNA ends such as that required for double-strand break repair, transposition, and V(D)J recombination. High levels of autoantibodies to p70 and p80 have been found in some patients with systemic lupus erythematosus.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

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引用该产品

生物信息学

蛋白别名: 5'-deoxyribose-5-phosphate lyase Ku70; 5'-dRP lyase Ku70; 70 kDa subunit of Ku antigen; ATP-dependent DNA helicase 2 subunit 1; ATP-dependent DNA helicase II 70 kDa subunit; ATP-dependent DNA helicase II, 70 kDa subunit; CT; CTA-216E10.7; CTC box binding factor 75 kDa subunit; CTC box-binding factor 75 kDa subunit; CTC75; DNA repair protein XRCC6; Ku autoantigen p70 subunit; Ku autoantigen, 70kDa; Ku70; Lupus Ku autoantigen protein p70; OTTHUMP00000028581; thyroid autoantigen 70kD (Ku antigen); thyroid autoantigen 70kDa (Ku antigen); Thyroid-lupus autoantigen; thyroid-lupus autoantigen p70; TLAA; X-ray repair complementing defective repair in Chinese hamster cells 6; X-ray repair cross-complementing protein 6

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基因别名: CTC75; CTCBF; G22P1; KU70; ML8; TLAA; XRCC6

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UniProt ID: (Human) P12956

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Entrez Gene ID: (Human) 2547

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功能