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Invitrogen
Ku70 Monoclonal Antibody (N3H10)
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Ku70 Antibody (MA5-13110) in ICC/IF
Immunofluorescence analysis of Ku70 was performed using 70% confluent log phase HeLa cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 1% BSA for 1 hour at room temperature. The cells were labeled with Ku70 Monoclonal Antibody (N3H10) (Product # MA5-13110) at 1:100 dilution in 0.1% BSA, incubated at 4 degree Celsius overnight and then labeled with Goat anti-Mouse IgG (H+L) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate (Product # A28175) ... View More
产品信息
MA5-13110
应用
建议稀释比
已发表文章
产品规格
已发表种属
Hamster,
Human,
Mouse
宿主/亚型
Mouse
/ IgG2b
分类
Monoclonal
类型
Antibody
克隆号
N3H10
抗原
Human placental extract designated as PSE1-PL
偶联物
形式
Liquid
浓度
0.2 mg/mL
保存条件
4°C
运输条件
Wet ice
RRID
产品详细信息
MA5-13110 targets Ku (p70) in immunofluorescence, immunohistochemistry (paraffin), immunoprecipitation, FACS and Western blot applications and shows reactivity with Human, Non-human primate, and Xenopus laevis samples. Reacts with mouse and hamster in Western blot applications only.
The MA5-13110 immunogen is human placental extract designated as PSE1-PL.
Antibodies to this protein (and modification) were previously sold as part of a Thermo Scientific Cellomics High Content Screening Kit. This replacement antibody is now recommended for researchers who need an antibody for high content cell based assays. It has been thoroughly tested and validated for cellular immunofluorescence (IF) applications. Further optimization including the selection of the most appropriate fluorescent Dylight conjugated secondary antibody may have to be performed for your high content assay.
靶标信息
XRCC6 is a nuclear complex consisting of two subunits with molecular masses of approximately 70 and 80 kDa. The complex functions as a single-stranded DNA-dependent ATP-dependent helicase. The complex may be involved in the repair of nonhomologous DNA ends such as that required for double-strand break repair, transposition, and V(D)J recombination. High levels of autoantibodies to p70 and p80 have been found in some patients with systemic lupus erythematosus.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
