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LATS1 Antibody (711149) in ICC/IF
For immunofluorescence analysis, SH-SY5Y cells were fixed and permeabilized for detection of endogenous LATS1 using LATS1 Recombinant Rabbit Polyclonal Antibody (Product # 711149, 2 µg/mL) and labeled with Goat anti-Rabbit IgG (Heavy Chain) Superclonal Secondary Antibody, Alexa Fluor® 488 conjugate (Product # A27034, 1:2000). Panel a) shows representative cells that were stained for detection and localization of LATS1 protein (green), Panel b) is stained for nuclei (blue) using SlowFade® Gold Antifade Mountant with DAPI (Product # S36938).... View More
产品信息
711149
产品规格
种属反应
Human
宿主/亚型
Rabbit
/ IgG
Expression System
Expi293
分类
Recombinant Superclonal
类型
Antibody
克隆号
2HCLC
抗原
Protein corresponding to Human LATS1 (aa 11-277)
偶联物
形式
Liquid
浓度
0.5 mg/mL
纯化类型
Protein A
保存液
PBS, pH 7.4
内含物
0.09% sodium azide
保存条件
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
运输条件
Wet ice
RRID
AB_2633126
产品详细信息
This antibody is predicted to react with Monkey, Horse and Dog.
Recombinant rabbit polyclonal antibodies are unique offerings from Thermo Fisher Scientific. They are comprised of a selection of multiple different recombinant monoclonal antibodies, providing the best of both worlds - the sensitivity of polyclonal antibodies with the specificity of monoclonal antibodies - all delivered with the consistency only found in a recombinant antibody. While functionally the same as a polyclonal antibody - recognizing multiple epitope sites on the target and producing higher detection sensitivity for low abundance targets - a recombinant rabbit polyclonal antibody has a known mixture of light and heavy chains. The exact population can be produced in every lot, circumventing the biological variability typically associated with polyclonal antibody production.
靶标信息
LATS1 is a putative serine/threonine kinase that localizes to the mitotic apparatus and complexes with cell cycle controller CDC2 kinase in early mitosis which is phosphorylated in a cell-cycle dependent manner. The N-terminal region of the protein binds CDC2 to form a complex showing reduced H1 histone kinase activity which indicating a role as a negative regulator of CDC2/cyclin A and the C-terminal kinase domain binds to its own N-terminal region, suggesting potential negative regulation through interference with complex formation via intramolecular binding. LATS1 act as a tumor suppressor and play an important role in the development of soft-tissue sarcomas, ovarian stromal cell tumors and a high sensitivity to carcinogenic treatments.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
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生物信息学
蛋白别名: h-warts; Large tumor suppressor homolog 1; LATS (large tumor suppressor, Drosophila) homolog 1; LATS, large tumor suppressor, homolog 1; LATS1; Serine/threonine-protein kinase LATS1; WARTS protein kinase
基因别名: LATS1; WARTS; wts
UniProt ID: (Human) O95835
Entrez Gene ID: (Human) 9113
G1/S transition of mitotic cell cycle
G2/M transition of mitotic cell cycle
sister chromatid segregation
inner cell mass cell fate commitment
inner cell mass cellular morphogenesis
protein phosphorylation
mitotic nuclear division
hormone-mediated signaling pathway
keratinocyte differentiation
regulation of actin filament polymerization
positive regulation of peptidyl-serine phosphorylation
hippo signaling
positive regulation of apoptotic process
regulation of protein complex assembly
negative regulation of cyclin-dependent protein serine/threonine kinase activity
regulation of organ growth
cytoplasmic sequestering of protein
cell division
negative regulation of canonical Wnt signaling pathway
