Invitrogen

LC3A Polyclonal Antibody

高级验证

This Antibody was verified by Cell treatment to ensure that the antibody binds to the antigen stated.

1个已发表图片

2 篇参考文献

查看其他16个LC3A抗体

说明书

实验方案

技术支持

说明书

实验方案

技术支持

LC3A Antibody in Immunocytochemistry (ICC/IF)

图: 1 / 9

LC3A Antibody (PA5-22990) in ICC/IF

Immunocytochemistry analysis of LC3A in HeLa cells. Samples were incubated in LC3A polyclonal antibody (Product # PA5-22990) followed by DyLight 488 (green). Cells were treated overnight with 50 µM chloroquine to induce autophagosome formation. Nuclei and alpha-tubulin were counterstained with DAPI (blue) and DyLight 550 (red).

LC3A Antibody in Immunohistochemistry (Paraffin) (IHC (P))

LC3A Antibody in Immunohistochemistry (IHC)

Product Image Gallery

LC3A Antibody — Altered expression of proteins upon cell treatment demonstrates antibody specificity. Western blot using Anti-LC3A Polyclonal Antibody (Product # PA5-22990), shows induced expression in HeLa and HCT 116 cells upon Chloroquine treatment. {TM}

产品信息

PA5-22990

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1:1,000-1:2,500

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免疫组化 (IHC)

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免疫组化（石蜡） (IHC (P))

1:100-1:400

免疫组化（冰冻） (IHC (F))

Assay-Dependent

免疫细胞化学 (ICC/IF)

1:100

流式细胞分析 (Flow)

1:100

产品规格

种属反应

Bovine, Human, Mouse, Rat, Zebrafish

已发表种属

Mouse

宿主/亚型

Rabbit / IgG

分类

Polyclonal

类型

Antibody

抗原

Genomic sequence made to a N-terminal portion of the human LC3A protein.

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偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

1.0 mg/mL

纯化类型

Antigen affinity chromatography

保存液

PBS

内含物

0.02% sodium azide

保存条件

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

运输条件

Ambient (domestic); Wet ice (international)

RRID

AB_11154460

产品详细信息

In ICC/IF, autophagosome formation has been seen in HeLa cells after treatment with 50uM chloroquine.

Suggested positive control: human brain lysate, LC3 overexpression lysate.

靶标信息

MAP1A and MAP1B are microtubule-associated proteins which mediate the physical interactions between microtubules and components of the cytoskeleton. MAP1A and MAP1B each consist of a heavy chain subunit and multiple light chain subunits. The protein encoded by this gene is one of the light chain subunits and can associate with either MAP1A or MAP1B. Two transcript variants encoding different isoforms have been found for this gene.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

Product References

Involvement of the TNF-α/SATB2 axis in the induced apoptosis and inhibited autophagy of osteoblasts by the antipsychotic Risperidone.

Molecular medicine (Cambridge, Mass.)

Zhang S,He W,Li A,Zhao C,Chen Y,Xu C,Zhang Q,Zheng D,Chen M,Miao H,Huang Y

Published figure using LC3A polyclonal antibody (Product # PA5-22990) in Immunohistochemistry

Tue May 03 00:00:00 EDT 2022

Diethylstilbestrol (DES) induces autophagy in thymocytes by regulating Beclin-1 expression through epigenetic modulation.

Toxicology

Singh NP,Miranda K,Singh UP,Nagarkatti P,Nagarkatti M

PA5-22990 was used in Western Blotting to demonstrate for the first time that DES induces autophagy in thymocytes potentially through epigenetic changes involving hypomethylation of Becn1 and down-regulation of miR-30a expression.

Sat Dec 01 00:00:00 EST 2018

生物信息学

蛋白别名: Autophagy-related protein LC3 A; Autophagy-related ubiquitin-like modifier LC3 A; MAP1 light chain 3-like protein 1; MAP1A/1B light chain 3 A; MAP1A/MAP1B LC3 A; MAP1A/MAP1B light chain 3 A; Microtubule-asso; Microtubule-associated protein 1 light chain 3 alpha; microtubule-associated protein 1-light chain 3A; microtubule-associated proteins 1A/1B light chain 3; Microtubule-associated proteins 1A/1B light chain 3A; MLP3A; RP11-346K17.1

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基因别名: 1010001H21Rik; 4922501H04Rik; ATG8E; LC3; LC3A; MAP1ALC3; MAP1BLC3; MAP1LC3A; zgc:77094