Invitrogen

LDHA/LDHB Recombinant Rabbit Monoclonal Antibody (PSH12-75)

Name: LDHA\/LDHB Recombinant Rabbit Monoclonal Antibody (PSH12-75)
Brand: Invitrogen
SKU: MA569999

说明书

证书

实验方案

问题与答案

技术支持

说明书

证书

实验方案

问题与答案

技术支持

说明书

证书

实验方案

问题与答案

技术支持

抗体检测数据 (13)

LDHA/LDHB Antibody in Immunocytochemistry (ICC/IF)

图: 1 / 13

LDHA/LDHB Antibody (MA569999) in ICC/IF

Immunocytochemistry analysis of LDHA/LDHB in PC-12 cells. Samples were incubated with LDHA/LDHB monoclonal antibody (Product # MA569999) using a dilution of 1:100 in 1% BSA in PBST overnight at 4°C followed by Goat Anti-Rabbit IgG H&L (iFluor™ 488) at a dilution of 1:1,000. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes ... View More

LDHA/LDHB Antibody in Immunohistochemistry (Paraffin) (IHC (P))

LDHA/LDHB Antibody in Flow Cytometry (Flow)

LDHA/LDHB Antibody in Immunoprecipitation (IP)

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Immunogen image

Immunogen Image LDHA/LDHB Recombinant Rabbit Monoclonal Antibody (PSH12-75)

产品信息

MA569999

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1:5,000

免疫组化（石蜡） (IHC (P))

1:20,000

免疫细胞化学 (ICC/IF)

1:100

流式细胞分析 (Flow)

1:1,000

免疫沉淀 (IP)

1-2 µg

产品规格

种属反应

Rat, Human, Mouse

宿主/亚型

Rabbit / IgG

Expression System

HEK293 cells

分类

Recombinant Monoclonal

类型

Antibody

克隆号

PSH12-75

抗原

Synthetic peptide within Human LDHA aa 1-50.

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偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

1 mg/mL

纯化类型

Protein A

保存液

PBS, pH 7.4, with 0.1% BSA, 40% glycerol

内含物

0.05% sodium azide

保存条件

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

运输条件

Ambient (domestic); Wet ice (international)

产品详细信息

Positive control: HepG2 cell lysate, HeLa cell lysate, MCF7 cell lysate, A431 cell lysate, 293T cell lysate, PANC-1 cell lysate, U-87 MG cell lysate, MDA-MB-231 cell lysate, NIH/3T3 cell lysate, RAW264.7 cell lysate, Neuro-2a cell lysate, C2C12 cell lysate, C6 cell lysate, PC-12 cell lysate, human breast cancer tissue, human liver tissue, mouse liver tissue, mouse heart tissue, rat heart tissue.

Subcellular location: Cytoplasm.

靶标信息

Both LDHA and LDHB genes are part of the lactate dehydrogenase enzyme family, functioning together to regulate lactate metabolism. These genes show differential expression in tissues, with LDHA being more prominent in skeletal muscle, whereas LDHB is prevalent in heart muscle. Lactate dehydrogenase A (LDHA) is a gene located on chromosome 11p15.4. It encodes for the A subunit of the enzyme lactate dehydrogenase (LDH), which is crucial in the conversion of pyruvate to lactate during anaerobic glycolysis. The LDHA gene is involved in metabolic processes and plays a role in muscle function. Overexpression of LDHA is often associated with various cancers, highlighting its significance in metabolic reprogramming of cancer cells. Lactate dehydrogenase B (LDHB) is located on chromosome 12p12.2. It encodes for the B subunit of LDH, which predominantly facilitates the conversion of lactate to pyruvate under aerobic conditions. The LDHB gene is widely expressed in the heart, brain, and kidneys, where it helps maintain cellular energy homeostasis. LDHB serves as a biomarker in certain diseases, including cardiac conditions, where its serum levels are measured to assess tissue damage.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

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生物信息学

蛋白别名: Cell proliferation-inducing gene 19 protein; epididymis secretory protein Li 281; epididymis secretory sperm binding protein Li 133P; L-lactate dehydrogenase A chain; L-lactate dehydrogenase B chain; L-lactate dehydrogenase C chain; lactate dehydrogenase 1, A chain; lactate dehydrogenase 3, C chain, sperm specific; lactate dehydrogenase A-like; lactate dehydrogenase A4; lactate dehydrogenase C variant 3; lactate dehydrogenase H chain; lactate dehydrogenase M; lactate dehydrogenase-A; Lactate dehydrogenease B; LDH heart subunit; LDH muscle subunit; LDH testis subunit; LDH-A; LDH-B; LDH-C; LDH-H; LDH-M; LDH-X; M-LDH; proliferation-inducing gene 19; Renal carcinoma antigen NY-REN-46; Renal carcinoma antigen NY-REN-59; testicular secretory protein Li 25

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基因别名: AI790582; GSD11; H-Ldh; HEL-S-133P; HEL-S-281; l7R2; Ldh-1; Ldh-2; LDH-B; LDH-H; Ldh1; Ldh2; Ldh3; LDHA; LDHB; LDHBD; LDHM; PIG19; TRG-5