Invitrogen

LRRK2 Monoclonal Antibody (N138/6), FITC

查看其他23个LRRK2抗体

抗体检测数据 (3)

LRRK2 Antibody in Immunocytochemistry (ICC/IF)

LRRK2 Antibody in Immunocytochemistry (ICC/IF)

图: 1 / 3

LRRK2 Antibody (MA5-45645) in ICC/IF

Immunocytochemistry/Immunofluorescence analysis using human neuroblastoma cells. Fixation involved 4% Formaldehyde for 15 min at RT. Samples were incubated with LRRK2/Dardarin monoclonal antibody (Product # MA5-45645) at 1:100 for 60 min at RT, followed by Goat Anti-Mouse ATTO 488 at 1:100 for 60 min at RT. Counterstain used was Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1,000; 1:5,000 for 60 min RT, 5 min RT. Localization: Cytoplasm, Membrane, Nucleus. Magnification: 60X. (A) DAPI (blue) nuclear stain. (B) Phalloid... View More

LRRK2 Antibody in Immunocytochemistry (ICC/IF)

LRRK2 Antibody in Immunocytochemistry (ICC/IF)

LRRK2 Antibody in Western Blot (WB)

Product Image Gallery

LRRK2 Antibody in Immunocytochemistry (ICC/IF) — Immunocytochemistry/Immunofluorescence analysis using human neuroblastoma cells. Fixation involved 4% Formaldehyde for 15 min at RT. Samples were incubated with LRRK2/Dardarin monoclonal antibody (Product # MA5-45645) at 1:100 for 60 min at RT, followed by Goat Anti-Mouse ATTO 488 at 1:100 for 60 min at RT. Counterstain used was Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1,000; 1:5,000 for 60 min RT, 5 min RT. Localization: Cytoplasm, Membrane, Nucleus. Magnification: 60X. (A) DAPI (blue) nuclear stain. (B) Phalloidin Texas Red F-Actin stain. (C) LRRK2/Dardarin antibody. (D) Composite.

LRRK2 Antibody in Immunocytochemistry (ICC/IF) — Immunocytochemistry/Immunofluorescence analysis using human neuroblastoma cells. Fixation involved 4% PFA for 15 min. Samples were incubated with LRRK2/Dardarin monoclonal antibody (Product # MA5-45645) at 1:100 for overnight at 4°C with slow rocking, followed by AlexaFluor 488 at 1:1,000 for 1 hour at RT. Counterstain used was Phalloidin-iFluor 647 (red) F-Actin stain; Hoechst (blue) nuclear stain at 1:800, 1.6mM for 20 min at RT. (A) Hoechst (blue) nuclear stain. (B) Phalloidin-iFluor 647 (red) F-Actin stain. (C) LRRK2/Dardarin antibody (D) Composite.

LRRK2 Antibody in Western Blot (WB) — Western Blot analysis of Rat Brain Membrane showing detection of >200 kDa LRRK2/Dardarin protein. Lane 1: Molecular Weight Ladder. Lane 2: Rat Brain Membrane. Load: 15 µg. Blocking: 2% BSA and 2% Skim Milk in 1X TBST. Samples were incubated with LRRK2/Dardarin monoclonal antibody (Product # MA5-45645) at 1:200 for 16 hours at 4°C, followed by Goat Anti-Mouse IgG: HRP at 1:1,000 for 1 hour RT. Color Development: ECL solution for 6 min in RT. Predicted/Observed Size: >200 kDa.

产品信息

MA5-45645

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1:1,000

-

免疫细胞化学 (ICC/IF)

Assay-dependent

-

产品规格

种属反应

Human, Mouse, Rat

宿主/亚型

Mouse / IgG1

分类

Monoclonal

类型

Antibody

克隆号

N138/6

抗原

Fusion protein, amino acids 1-500 (N-terminus) of human LRRK2.

查看免疫原

偶联物

FITC FITC FITC

激发/发射光谱

498/517 nm 查看光谱

形式

Liquid

浓度

1 mg/mL

纯化类型

Protein G

保存液

9.1mM sodium bicarbonate/PBS, pH 7.4, with 581mM DMSO, 123mM ethanolamine

内含物

no preservative

保存条件

4° C, store in dark

运输条件

Ambient (domestic); Wet ice (international)

RRID

AB_2932099

产品详细信息

83% identical in mouse and rat. No significant identity with LRRK1.

1 µg/mL of MA5-45645 was sufficient for detection of LRRK2/Dardarin in 20 µg of rat brain lysate by colorimetric immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody.|Detects >200kDa.

This antibody was formerly sold as clone S138-6.

靶标信息

LRRK2 is a multidomain protein that includes a kinase domain, a GTPase domain, and a leucine-rich repeat (LRR) domain. Mutations in LRRK2 have been associated with Parkinson's disease. LRRK2 is a member of the leucine-rich repeat kinase family and encodes a protein with an ankryin repeat region, a leucine-rich repeat domain, a kinase domain, a DFG-like motif, a RAS domain, a GTPase domain, a MLK-like domain, and a WD40 domain. The protein is present largely in the cytoplasm but also associates with the mitochondrial outer membrane.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

篇参考文献 (0)

您是否在文献中引用过该产品？请点击下方按钮邮件告知我们。

引用该产品

生物信息学

蛋白别名: augmented in rheumatoid arthritis 17; BC 300-268; Dardarin; DKFZp434H2111; FLJ45829; leucine-rich repeat kinase 2; Leucine-rich repeat serine/threonine-protein kinase 2; LRR kinase 2; LRRK 2; LRRK-2

查看更多收起

基因别名: 4921513O20Rik; 9330188B09Rik; AURA17; AW561911; cI-46; D630001M17Rik; DARDARIN; Gm927; LRRK2; PARK8; RIPK7; ROCO2

查看更多收起

UniProt ID: (Human) Q5S007, (Mouse) Q5S006

查看更多收起

Entrez Gene ID: (Human) 120892, (Mouse) 66725, (Rat) 300160

查看更多收起

SNARE binding glycoprotein binding actin binding GTPase activity protein kinase activity protein serine/threonine kinase activity MAP kinase kinase activity GTPase activator activity protein binding ATP binding GTP binding microtubule binding tubulin binding kinase activity Rho GTPase binding syntaxin-1 binding receptor signaling complex scaffold activity clathrin binding GTP-dependent protein kinase activity peroxidase inhibitor activity co-receptor binding identical protein binding protein homodimerization activity ion channel binding protein kinase A binding beta-catenin destruction complex binding nucleotide binding transferase activity transferase activity, transferring phosphorus-containing groups molecular_function scaffold/adaptor protein

参与通路

MAPK cascade activation of MAPKK activity activation of MAPK activity negative regulation of protein phosphorylation positive regulation of protein phosphorylation protein phosphorylation endocytosis autophagy response to oxidative stress mitochondrion organization Golgi organization lysosome organization small GTPase mediated signal transduction neuromuscular junction development determination of adult lifespan cellular response to starvation regulation of autophagy positive regulation of autophagy regulation of protein kinase A signaling negative regulation of protein processing regulation of neuron maturation negative regulation of macroautophagy phosphorylation peptidyl-serine phosphorylation peptidyl-threonine phosphorylation calcium-mediated signaling olfactory bulb development tangential migration from the subventricular zone to the olfactory bulb positive regulation of protein ubiquitination negative regulation of protein binding positive regulation of protein binding positive regulation of proteasomal ubiquitin-dependent protein catabolic process negative regulation of GTPase activity cellular response to oxidative stress cellular protein localization intracellular signal transduction regulation of kidney size exploration behavior locomotory exploration behavior regulation of lysosomal lumen pH regulation of locomotion regulation of membrane potential positive regulation of programmed cell death positive regulation of MAP kinase activity positive regulation of GTPase activity GTP metabolic process protein autophosphorylation intracellular distribution of mitochondria neuron projection morphogenesis regulation of calcium-mediated signaling mitochondrion localization regulation of mitochondrial depolarization regulation of synaptic transmission, glutamatergic canonical Wnt signaling pathway excitatory postsynaptic potential regulation of dopamine receptor signaling pathway positive regulation of dopamine receptor signaling pathway regulation of canonical Wnt signaling pathway regulation of dendritic spine morphogenesis protein localization to mitochondrion neuron death cellular response to manganese ion reactive oxygen species metabolic process regulation of mitochondrial fission positive regulation of canonical Wnt signaling pathway negative regulation of excitatory postsynaptic potential regulation of neuron death negative regulation of neuron death negative regulation of endoplasmic reticulum stress-induced intrinsic apoptotic signaling pathway positive regulation of protein autoubiquitination regulation of neuroblast proliferation regulation of synaptic vesicle transport negative regulation of late endosome to lysosome transport negative regulation of autophagosome assembly negative regulation of thioredoxin peroxidase activity by peptidyl-threonine phosphorylation negative regulation of hydrogen peroxide-induced cell death negative regulation of protein targeting to mitochondrion negative regulation of protein processing involved in protein targeting to mitochondrion cellular response to dopamine Wnt signalosome assembly regulation of retrograde transport, endosome to Golgi regulation of branching morphogenesis of a nerve regulation of synaptic vesicle exocytosis negative regulation of peroxidase activity cell differentiation synaptic vesicle recycling cellular response to organic cyclic compound