MUbio

Lamin B1 Monoclonal Antibody (119D5-F1)

Name: Lamin B1 Monoclonal Antibody (119D5-F1)
Brand: MUbio
SKU: MUB1103P

查看其他34个Lamin B1抗体

说明书

实验方案

问题与答案

技术支持

说明书

实验方案

问题与答案

技术支持

说明书

实验方案

问题与答案

技术支持

产品信息

MUB1103P

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1:100-1:1,000

免疫组化（冰冻） (IHC (F))

1:100-1:200

免疫细胞化学 (ICC/IF)

1:100-1:200

流式细胞分析 (Flow)

1:100-1:200

酶联免疫吸附实验 (ELISA)

Assay-dependent

产品规格

种属反应

Bovine, Dog, Human, Mouse, Sheep, Rabbit, Rat, Zebrafish

宿主/亚型

Mouse / IgG1

分类

Monoclonal

类型

Antibody

克隆号

119D5-F1

抗原

Lamin B1

偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

1 mg/mL

纯化类型

Protein A/G

保存液

PBS

内含物

0.09% sodium azide

保存条件

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

运输条件

Ambient (domestic); Wet ice (international)

产品详细信息

119D5-F1 reacts with an epitope located C-terminal of residue 231 in lamin B1.

The antibody may be stored at 4°C. For prolonged storage prepare appropriate aliquots and store at or below -20°C. Prior to use, an aliquot is thawed slowly in the dark at ambient temperature, spun down again and used to prepare working dilutions by adding sterile phosphate buffered saline (PBS, pH 7.2). Repeated thawing and freezing should be avoided. Working dilutions should be stored at 4°C, not refrozen, and preferably used the same day. If a slight precipitation occurs upon storage, this should be removed by centrifugation. It will not affect the performance or the concentration of the product.

靶标信息

Lamin B1 is a key member of the nuclear lamina structure, encoded by the LMNB1 gene. It is part of the B-type lamins, ubiquitously expressed across all mammalian cells, providing structural integrity and mechanical support by forming a meshwork along the inner nuclear membrane. Lamin B1 is involved in maintaining the shape and stability of the nucleus, playing a critical role in cellular processes such as genome organization, DNA replication, and transcription regulation. The lamin filaments are type V intermediate filament proteins, enabling the nucleus to withstand mechanical stress and preserve its functionality. Lamin B1's expression and dynamics vary during cell differentiation, influencing gene regulation. Its location within the euchromatin-enriched section of the genome suggests potential roles in chromatin organization and the epithelial-to-mesenchymal transition. Governed by complex interactions with other proteins, lamin B1 is integral to cellular architecture and health, with variations in its levels linked to developmental and disease processes. Recent studies have highlighted the importance of understanding the role and dynamics of lamin B1 in human-induced pluripotent stem cells and germ layer cells, offering insights into differentiation-dependent changes and their implications for nuclear structure.

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