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Lck Antibody (HA500280) in WB
Western blot analysis of Lck on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA500280, 1:1,000) was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:200,000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: Rat thymus tissue lysate Lane 2: Jurkat cell lysate.
产品信息
HA500280
产品规格
种属反应
Human,
Rat
宿主/亚型
Rabbit
/ IgG
分类
Polyclonal
类型
Antibody
抗原
Recombinant protein within human Lck aa 100-300/509.
偶联物
Unconjugated
形式
Liquid
浓度
1 mg/mL
纯化类型
Antigen affinity chromatography
保存液
TBS, pH 7.4, with 0.2% BSA, 50% glycerol
内含物
0.05% sodium azide
保存条件
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
运输条件
Wet ice
靶标信息
LCK is a member of the Src-family tyrosine kinase, which are essential for signaling through the T-cell receptor (TCR) complex. Upon TCR triggering, LCK phosphorylates the ITAM motives in its zeta subunits establishing binding sites for the SH2 domains of the tyrosine kinase ZAP70, which is also phosphorylated by LCK and activated to generate subsequent signaling platforms by phosphorylation of adaptor LAT. The majority of LCK is localized to the plasma membrane, however, there is also a significant fraction associated with the Golgi apparatus which may contribute to Raf activation under conditions of weak stimulation through the TCR. LCK contains N-terminal sites for myristylation and palmitylation, a PTK domain, and SH2 and SH3 domains which are involved in mediating protein-protein interactions with phosphotyrosine-containing and proline-rich motifs, respectively. LCK is also involved in the regulation of apoptosis induced by various stimuli, but not by the death receptors. Diseases associated with LCK include Immunodeficiency 22 and CD45 deficiency. Alternatively splice variants of the LCK gene encoding different isoforms of the protein have been found.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
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生物信息学
蛋白别名: EC 2.7.10.2; kinase Lck; Leukocyte C-terminal Src kinase; LSK; Lymphocyte cell-specific protein-tyrosine kinase; lymphocyte protein tyrosine kinase; lymphocyte-specific protein tyrosine kinase; OTTHUMP00000008640; OTTHUMP00000008740; OTTHUMP00000008741; p56(LSTRA) protein-tyrosine kinase; p56-LCK; Protein YT16; Proto-oncogene Lck; Proto-oncogene tyrosine-protein kinase LCK; RP4-675E8.4; T cell-specific protein-tyrosine kinase; T-lymphocyte specific protein tyrosine kinase p56lck; tyr; Tyrosine-protein kinase Lck
基因别名: IMD22; LCK; Lck1; Lcktkr; LSK; p56lck; pp58lck; YT16
UniProt ID: (Human) P06239, (Rat) Q01621
Entrez Gene ID: (Human) 3932, (Rat) 313050
glycoprotein binding
protein tyrosine kinase activity
non-membrane spanning protein tyrosine kinase activity
protein serine/threonine phosphatase activity
protein binding
ATP binding
protein C-terminus binding
kinase activity
protein kinase binding
protein phosphatase binding
SH2 domain binding
CD4 receptor binding
CD8 receptor binding
identical protein binding
phosphatidylinositol 3-kinase binding
phosphatidylinositol-4,5-bisphosphate 3-kinase activity
ATPase binding
antigen binding
receptor binding
protein complex binding
protein phosphorylation
cellular zinc ion homeostasis
apoptotic process
activation of cysteine-type endopeptidase activity involved in apoptotic process
cell surface receptor signaling pathway
transmembrane receptor protein tyrosine kinase signaling pathway
aging
response to mechanical stimulus
response to metal ion
response to zinc ion
positive regulation of gene expression
dephosphorylation
peptidyl-tyrosine phosphorylation
T cell differentiation
peptidyl-tyrosine autophosphorylation
regulation of cell proliferation
response to drug
positive regulation of tyrosine phosphorylation of Stat5 protein
response to hydrogen peroxide
regulation of apoptotic process
innate immune response
positive regulation of gamma-delta T cell differentiation
protein autophosphorylation
B cell receptor signaling pathway
regulation of T cell receptor signaling pathway
positive regulation of T cell receptor signaling pathway
positive regulation of T cell activation
release of sequestered calcium ion into cytosol
positive regulation of uterine smooth muscle contraction
cellular response to peptide hormone stimulus
positive regulation of intrinsic apoptotic signaling pathway
protein dephosphorylation
regulation of phosphatidylinositol 3-kinase signaling
viral process
hemopoiesis
platelet activation
T cell costimulation
phosphatidylinositol phosphorylation
phosphatidylinositol-mediated signaling
regulation of defense response to virus by virus
T cell receptor signaling pathway
leukocyte migration
regulation of lymphocyte activation
