Invitrogen

Ly-49H Monoclonal Antibody (3D10), APC, eBioscience™

Ly-49H Antibody in Flow Cytometry (Flow)

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Ly-49H Antibody (17-5886-82) in Flow

Staining of C57Bl/6 splenocytes with Anti-Mouse CD49b (Integrin alpha 2) PE (Product # 12-5971-82) and 0.5 µg of Mouse IgG1 K Isotype Control APC (Product # 17-4714-81) (left) or 0.5 µg of Anti-Mouse Ly-49H APC (right). Cells in the lymphocyte gate were used for analysis.

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产品信息

17-5886-82

应用

建议稀释比

已发表文章

流式细胞分析 (Flow)

1 µg/test

查看11篇已发表文章 11篇已发表文章

产品规格

种属反应

Mouse

已发表种属

Mouse

宿主/亚型

Mouse / IgG1, kappa

分类

Monoclonal

类型

Antibody

克隆号

3D10

偶联物

APC APC APC

激发/发射光谱

651/660 nm 查看光谱

形式

Liquid

浓度

0.2 mg/mL

纯化类型

Affinity chromatography

保存液

PBS, pH 7.2

内含物

0.09% sodium azide

保存条件

4°C, store in dark, DO NOT FREEZE!

运输条件

Ambient (domestic); Wet ice (international)

AB_10598809

产品详细信息

Description: The monoclonal antibody 3D10 recognizes Ly-49H, a member of the C-type lectin Ly-49 multigene family of receptors found on natural killer cells. Ly-49H, like Ly-49D, lacks the characteristic ITIM (immunoreceptor tyrosine-based inhibitory motif) and contains an arginine in the transmembrane domain implying these molecules act as activation receptors. Ly-49H is expressed in a subset of NK cells but not NKT (NK1.1CD3+) cells. Natural killer cells can express several Ly-49 proteins. It has been shown that there is preferential staining of Ly-49H with Ly-49D (Product # 00-5523-00) and protocol. Please see BestProtocols® Section (Staining intracellular Antigens for Flow Cytometry) for staining protocol (refer to Protocol B: One-step protocol for intracellular (nuclear) proteins). This can be used at less than or equal to 0.125 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

Excitation: 488 nm; Emission: 520 nm; Laser: Blue Laser.

Filtration: 0.2 µm post-manufacturing filtered.

靶标信息

Ly49H is a member of the C-type lectin Ly-49 multigene family of receptors found on natural killer cells. Ly49H, like Ly49D, lacks the characteristic ITAM (immunoreceptor tyrosine-based inhibitory motif) and contains an arginine in the transmembrane domain implying these molecules act as activation receptors. Ly49H is expressed in a subset of NK cells but not NKT (NK1.1/CD3+) cells. Natural killer cells can express several Ly49 proteins.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

How to use the Panel Builder

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Product References

Perinatal murine cytomegalovirus infection reshapes the transcriptional profile and functionality of NK cells.

Nature communications

Rožmanić C,Lisnić B,Pribanić Matešić M,Mihalić A,Hiršl L,Park E,Lesac Brizić A,Indenbirken D,Viduka I,Šantić M,Adler B,Yokoyama WM,Krmpotić A,Juranić Lisnić V,Jonjić S,Brizić I

Published figure using Ly-49H monoclonal antibody (Product # 17-5886-82) in Flow Cytometry

Thu Oct 12 00:00:00 UTC 2023

Canonical IRE1 function needed to sustain vigorous natural killer cell proliferation during viral infection.

iScience

Vetters J,van Helden M,De Nolf C,Rennen S,Cloots E,Van De Velde E,Fayazpour F,Van Moorleghem J,Vanheerswynghels M,Vergote K,Boon L,Vivier E,Lambrecht BN,Janssens S

Published figure using Ly-49H monoclonal antibody (Product # 17-5886-82) in Flow Cytometry

Fri Dec 15 00:00:00 UTC 2023

PD-1 expression on mouse intratumoral NK cells and its effects on NK cell phenotype.

iScience

Wagner AK,Kadri N,Tibbitt C,van de Ven K,Bagawath-Singh S,Oliinyk D,LeGresley E,Campbell N,Trittel S,Riese P,Ribacke U,Sandalova T,Achour A,Kärre K,Chambers BJ

17-5886-82 was used in Flow cytometry/Cell sorting to demonstrate that there may be a role for the PD-1/PD-L1 axis in tumor-infiltrating NK cells in vivo.

Fri Oct 21 00:00:00 UTC 2022

Control of Viral Infection by Natural Killer Cell Inhibitory Receptors.

Cell reports

Parikh BA,Bern MD,Piersma SJ,Yang L,Beckman DL,Poursine-Laurent J,Plougastel-Douglas B,Yokoyama WM

17-5886 was used in Flow cytometry/Cell sorting to show MHC-I-restricted control of murine cytomegalovirus (MCMV) infection in vivo that is NK cell dependent.

Tue Jul 28 00:00:00 UTC 2020

Hhex Directly Represses BIM-Dependent Apoptosis to Promote NK Cell Development and Maintenance.

Cell reports

Goh W,Scheer S,Jackson JT,Hediyeh-Zadeh S,Delconte RB,Schuster IS,Andoniou CE,Rautela J,Degli-Esposti MA,Davis MJ,McCormack MP,Nutt SL,Huntington ND

17-5886 was used in Flow cytometry/Cell sorting to show homeobox transcriptional regulator Hhex regulates NK cell homeostasis and immunity.

Tue Oct 20 00:00:00 UTC 2020

Stage-Specific Requirement for Eomes in Mature NK Cell Homeostasis and Cytotoxicity.

Cell reports

Wagner JA,Wong P,Schappe T,Berrien-Elliott MM,Cubitt C,Jaeger N,Lee M,Keppel CR,Marin ND,Foltz JA,Marsala L,Neal CC,Sullivan RP,Schneider SE,Keppel MP,Saucier N,Cooper MA,Fehniger TA

17-5886 was used in Flow cytometry/Cell sorting to develop a tamoxifen-inducible, type-1-ILC-specific (Ncr1-targeted) cre mouse and combine this with Eomes-floxed mice.

Tue Jun 02 00:00:00 UTC 2020

Virus infection is controlled by hematopoietic and stromal cell sensing of murine cytomegalovirus through STING.

eLife

Piersma SJ,Poursine-Laurent J,Yang L,Barber GN,Parikh BA,Yokoyama WM

17-5886 was used in Flow cytometry/Cell sorting to contrast anti-viral effects of MyD88 versus STING in distinct cell types that are infected with murine CMV (MCMV).

Wed Jul 29 00:00:00 UTC 2020

Synergized regulation of NK cell education by NKG2A and specific Ly49 family members.

Nature communications

Zhang X,Feng J,Chen S,Yang H,Dong Z

17-5886 was used in Flow cytometry/Cell sorting to provide genetic evidence supporting that NKG2A and the inhibitory members of Ly49 family receptors synergize to regulate NK cell education.

Fri Nov 01 00:00:00 UTC 2019

Diacylglycerol Kinase ζ Is a Target To Enhance NK Cell Function.

Journal of immunology (Baltimore, Md. : 1950)

Yang E,Singh BK,Paustian AM,Kambayashi T

17-5886 was used in Flow cytometry/Cell sorting to investigate methods to enhance NK cell function, showing that diacylglycerol kinase ζ is a target for enhancement of NK function.

Mon Aug 01 00:00:00 UTC 2016

NK cell development requires Tsc1-dependent negative regulation of IL-15-triggered mTORC1 activation.

Nature communications

Yang M,Chen S,Du J,He J,Wang Y,Li Z,Liu G,Peng W,Zeng X,Li D,Xu P,Guo W,Chang Z,Wang S,Tian Z,Dong Z

17-5886 was used in Flow cytometry/Cell sorting to reveal a role for Tsc1-dependent inhibition of mTORC1 activation during immature NK cell development.

Wed Sep 07 00:00:00 UTC 2016

Markers of nonselective and specific NK cell activation.

Journal of immunology (Baltimore, Md. : 1950)

Fogel LA,Sun MM,Geurs TL,Carayannopoulos LN,French AR

17-5886 was used in Flow cytometry/Cell sorting to demonstrate that a combination of Sca-1, CD27, and KLRG1 can distinguish NK cells based on their mechanism of activation.