应用 | 建议稀释比 | 已发表文章 |
---|---|---|
流式细胞分析 (Flow) |
1 µg/test | 查看8篇已发表文章 8篇已发表文章 |
产品规格 | |
---|---|
种属反应 |
Mouse |
已发表种属 |
Mouse |
宿主/亚型 |
Mouse / IgG1, kappa |
推荐的同型对照 |
Mouse IgG1 kappa Isotype Control (P3.6.2.8.1), APC, eBioscience™ |
分类 |
Monoclonal |
类型 |
Antibody |
克隆号 |
3D10 |
偶联物 |
APC
|
激发/发射光谱 |
查看光谱 |
形式 |
Liquid |
浓度 |
0.2 mg/mL |
纯化类型 |
Affinity chromatography |
保存液 |
PBS, pH 7.2 |
内含物 |
0.09% sodium azide |
保存条件 |
4° C, store in dark, DO NOT FREEZE! |
RRID |
AB_10598809 |
Description: The monoclonal antibody 3D10 recognizes Ly-49H, a member of the C-type lectin Ly-49 multigene family of receptors found on natural killer cells. Ly-49H, like Ly-49D, lacks the characteristic ITIM (immunoreceptor tyrosine-based inhibitory motif) and contains an arginine in the transmembrane domain implying these molecules act as activation receptors. Ly-49H is expressed in a subset of NK cells but not NKT (NK1.1CD3+) cells. Natural killer cells can express several Ly-49 proteins. It has been shown that there is preferential staining of Ly-49H with Ly-49D (cat 12-5783). Expression has been confirmed on C57BL/6 and NWNA but not Balb/c or DBA/2 mice. Other mice strains have not been tested. No crossreactivity to Ly-49A, C, D or G2 has been observed. Addition of monoclonal antibody 3D10 to cell cultures induces lysis of target cells.
Applications Reported: This 3D10 antibody has been reported for use in flow cytometric analysis.
Applications Tested: This 3D10 antibody has been tested by flow cytometric analysis of C57Bl/6 spleen cells. This can be used at less than or equal to 1 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.
Excitation: 633-647 nm; Emission: 660 nm; Laser: Red Laser.
Filtration: 0.2 µm post-manufacturing filtered.
Ly49H is a member of the C-type lectin Ly-49 multigene family of receptors found on natural killer cells. Ly49H, like Ly49D, lacks the characteristic ITAM (immunoreceptor tyrosine-based inhibitory motif) and contains an arginine in the transmembrane domain implying these molecules act as activation receptors. Ly49H is expressed in a subset of NK cells but not NKT (NK1.1/CD3+) cells. Natural killer cells can express several Ly49 proteins.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
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蛋白别名: cytomegalovirus resistance 1; Killer cell lectin-like receptor 8; Ly-49h; Lymphocyte antigen 49h; T-cell surface glycoprotein Ly-49H
基因别名: Cmv-1; Cmv1; Klra8; Ly-49h; Ly49-h; Ly49H; Ly49u<129>
UniProt ID: (Mouse) Q60682
Entrez Gene ID: (Mouse) 16639