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          Clicking the images or links will redirect you to a website hosted by BenchSci that provides third-party scientific content. Neither the content nor the BenchSci technology and processes for selection have been evaluated by us; we are providing them as-is and without warranty of any kind, including for use or application of the Thermo Fisher Scientific products presented.

          • Primary Antibodies ›
          • MAP2 Antibodies

          Invitrogen

          MAP2 Monoclonal Antibody (M13)

          25个已发表图片
          61 篇参考文献
          查看其他34个MAP2抗体

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          说明书
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          说明书
          实验方案
          问题与答案
          技术支持

          Cite MAP2 Monoclonal Antibody (M13)

          • 抗体检测数据 (4)
          • 已发表图片 (25)
          MAP2 Antibody in Immunocytochemistry (ICC/IF)
          Group 53 Created with Sketch.
          MAP2 Antibody in Immunocytochemistry (ICC/IF)
          Group 53 Created with Sketch.

          图: 1 / 29

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          MAP2 Antibody (13-1500) in ICC/IF

          Immunofluorescent analysis of PSD-95 (green) and MAP2 (red) on rat primary cortical neurons cultured for 28 days in the B-27 Plus Neuronal Culture System (Product # A3653401). At day 28 the cells were fixed with 4% paraformaldehyde for 15 min, permeabilized with 0.1% triton x-100 for 30min, and blocked with 1% BSA for 30 min at room temperature. Cells were stained with anti-PSD95 antibody (Product # 51-6900) at a dilution of 1:200, and anti-MAP2 (Product # 13-1500) at a dilution of 1:400, in 1% BSA staining buffer, overnight at 4C, and the... View More {{ $ctrl.currentElement.advancedVerification.fullName }} 验证信息 View more
          已发表图片由___提供 benchsci-logo
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          查看产品

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          MAP2 Antibody in Immunocytochemistry (ICC/IF)
          MAP2 Antibody in Immunocytochemistry (ICC/IF)
          MAP2 Antibody in Immunohistochemistry (IHC)
          MAP2 Antibody in Western Blot (WB)
          MAP2 Antibody in Immunocytochemistry (ICC/IF)
          MAP2 Antibody in Immunocytochemistry (ICC/IF)
          MAP2 Antibody in Immunocytochemistry (ICC/IF)
          MAP2 Antibody in Immunocytochemistry (ICC/IF)
          MAP2 Antibody in Immunocytochemistry (ICC/IF)
          MAP2 Antibody in Immunocytochemistry (ICC/IF)
          MAP2 Antibody in Immunocytochemistry (ICC/IF)
          MAP2 Antibody in Immunocytochemistry (ICC/IF)
          MAP2 Antibody in Immunocytochemistry (ICC/IF)
          MAP2 Antibody in Immunocytochemistry (ICC/IF)
          MAP2 Antibody in Immunocytochemistry (ICC/IF)
          MAP2 Antibody in Immunocytochemistry (ICC/IF)
          MAP2 Antibody in Immunocytochemistry (ICC/IF)
          MAP2 Antibody in Immunocytochemistry (ICC/IF)
          MAP2 Antibody in Immunocytochemistry (ICC/IF)
          MAP2 Antibody in Immunocytochemistry (ICC/IF)
          MAP2 Antibody in Immunocytochemistry (ICC/IF)
          MAP2 Antibody in Immunocytochemistry (ICC/IF)
          MAP2 Antibody in Immunohistochemistry (IHC)
          MAP2 Antibody in Western Blot (WB)
          MAP2 Antibody in Western Blot (WB)
          MAP2 Antibody in Western Blot (WB)
          MAP2 Antibody in Western Blot (WB)
          MAP2 Antibody in Flow Cytometry (Flow)
          MAP2 Antibody in Flow Cytometry (Flow)

          产品信息

          13-1500

          应用
          建议稀释比
          已发表文章

          免疫印迹 (WB)

          1:500
          查看7篇已发表文章 7篇已发表文章

          免疫组化 (IHC)

          5-10 µg/mL
          查看10篇已发表文章 10篇已发表文章

          免疫组化(冰冻) (IHC (F))

          -
          查看1篇已发表文章 1篇已发表文章

          免疫细胞化学 (ICC/IF)

          1:250-1:400
          查看35篇已发表文章 35篇已发表文章

          流式细胞分析 (Flow)

          -
          查看3篇已发表文章 3篇已发表文章

          酶联免疫吸附实验 (ELISA)

          0.1-0.5 µg/mL
          查看2篇已发表文章 2篇已发表文章

          免疫沉淀 (IP)

          2-5 µg
          查看1篇已发表文章 1篇已发表文章

          其他PubMed (Misc)

          -
          查看2篇已发表文章 2篇已发表文章
          产品规格

          种属反应

          Human, Mouse, Rat

          已发表种属

          Horse, Human, Mouse, Rat

          宿主/亚型

          Mouse / IgG1, kappa

          分类

          Monoclonal

          类型

          Antibody

          克隆号

          M13

          抗原

          MAPs

          偶联物

          Unconjugated Unconjugated Unconjugated

          形式

          Liquid

          浓度

          0.5 mg/mL

          纯化类型

          purified

          保存液

          PBS, pH 7.4

          内含物

          0.1% sodium azide

          保存条件

          -20°C

          运输条件

          Ambient (domestic); Wet ice (international)

          RRID

          AB_2533001

          产品详细信息

          This antibody reacts with a 300 kD cytoskeletal protein (MAP2A and MAP2B) primarily expressed in the somatodendritic domain of the neuron but is also found in the glia. Reactivity is not affected by phospohorylation state of MAP2. Abnormal expression of MAP2 may cause cytoskeletal abnormalities and impair signal transduction in dendrites.

          This antibody is suitable for immunohistochemical staining of alcohol-fixed paraffin embedded or frozen tissue sections.

          靶标信息

          MAP2 (Microtubule Associated Protein 2) exists in two high molecular weight forms (MAP2a & MAP2b) and a low molecular weight form. The expression of MAP2 is developmentally regulated and its multiple forms arise by alternative splicing of a single gene. MAP2 is involved in microtubule assembly, which is an essential step in neurogenesis. The products of similar genes in rat and mouse are neuron-specific cytoskeletal proteins that are enriched in dendrites, implicating a role in determining and stabilizing dendritic shape during neuron development. MAP2 is useful in studies of neuron structure in normal and malignant brain tissue as well as in degenerative diseases such as Alzheimer's Disease (AD).

          仅用于科研。不用于诊断过程。未经明确授权不得转售。

          生物信息学

          蛋白别名: AC091465.1; DKFZp686I2148; MAP 2; MAP-2; map-2a; map2ab; Microtubule-associated protein 2; OTTHUMP00000163916; OTTHUMP00000208143

          查看更多 收起

          基因别名: G1-397-34; MAP-2; MAP2; MAP2A; MAP2B; MAP2C; MAP2R; Mtap-2; Mtap2; repro4

          查看更多 收起

          UniProt ID: (Human) P11137, (Mouse) P20357, (Rat) P15146

          查看更多 收起

          Entrez Gene ID: (Human) 4133, (Mouse) 17756, (Rat) 25595

          查看更多 收起

          功能
          dystroglycan binding structural molecule activity protein binding calmodulin binding microtubule binding cytoskeletal regulatory protein binding tubulin binding
          参与通路
          microtubule bundle formation axonogenesis dendrite development cellular response to organic substance microtubule cytoskeleton organization central nervous system neuron development neuron projection development dendrite morphogenesis response to estradiol response to progesterone
          It has to be done as per old AB suggested Products section.
          guarantee_icon

          性能保障

          只要您发现Invitrogen抗体在实验中的表现与网站或说明书的所述内容不符,您即可享受免费退换货服务。*

          了解更多
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