Invitrogen

MAST4 Polyclonal Antibody

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MAST4 Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry analysis of MAST4 in paraffin-embedded human rectum carcinoma tissue (cytoplasmic staining) and negative control (right, with PBS only). Samples were incubated with MAST4 polyclonal antibody (Product # PA5-36976) at a dilution of 1:50, followed by goat Anti-Rabbit IgG-biotin and avidin peroxidase.

MAST4 Antibody in Western Blot (WB) — Western blot analysis of MAST4 in Lane 1: HeLa cell lysate, Lane 2: sp2/0 cell lysate, Lane 3: PC12 cell lysate. Samples were incubated with MAST4 polyclonal antibody (Product # PA5-36976) at a dilution of 1:500.

产品信息

PA5-36976

应用	建议稀释比	已发表文章
免疫印迹 (WB)	1:500-1:1,000	-
免疫组化（石蜡） (IHC (P))	1:50-1:200	-
免疫细胞化学 (ICC/IF)	1:50-1:200	-
免疫沉淀 (IP)	1:50-1:200	-

产品规格
种属反应	Human, Mouse, Rat
宿主/亚型	Rabbit / IgG
分类	Polyclonal
类型	Antibody
抗原	Synthetic peptide corresponding to amino acids 108-153 of human MAST4
偶联物	Unconjugated
形式	Liquid
浓度	1 mg/mL
纯化类型	Antigen affinity chromatography
保存液	PBS, pH 7.2
内含物	15mM sodium azide
保存条件	Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
RRID	AB_2553845

产品详细信息

This antibody detects endogenous protein at a molecular weight of 266 kDa.

Purity is >95% by SDS-PAGE.

靶标信息

The phosphorylation and dephosphorylation of proteins on serine and threonine residues is an essential means of regulating a broad range of cellular functions in eukaryotes, including cell division, homeostasis and apoptosis. A group of proteins that are intimately involved in this process are the serine/threonine(Ser/Thr) protein kinases. MAST4 (microtubule associated serine/threonine kinase family member 4), also known as KIAA0303, is a 2,626 amino acid protein that localizes to the cytoplasm and contains one protein kinase domain, as well as one PDZ domain and one AGC-kinase C-terminal domain. Expressed at high levels in most adult tissues, including testis, colon and small intestine, MAST4 uses magnesium as a cofactor to catalyze the ATP-dependent phosphorylation of target proteins. Multiple isoforms of MAST4 exist due to alternative splicing events.

仅用于科研。不用于诊断过程。未经明确授权不得转售。