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MCP-4 Antibody (M810) in ELISA
Sandwich ELISA analysis of an anti-human MCP-4 monoclonal antibody (Product # M810) was performed by loading 100 µL per well of antibody (Product # M810) at a concentration of 2 µg/mL overnight at room temperature. The plate was washed 3 times with ELISA Wash Buffer (Product # N503), and 100 µL of recombinant human MCP-4/CCL13 was added to wells in duplicate at 2000, 1000, 500, 250, 125, 62.5, 31.25, and 0pg/mL concentrations and the samples were incubated for 2 hours at room temperature. The plate was washed, then incubated with 100 µL pe... View More
产品信息
M810
产品规格
种属反应
Human
宿主/亚型
Mouse
/ IgG1
分类
Monoclonal
类型
Antibody
克隆号
8C12
抗原
Recombinant CCL13 protein
偶联物
形式
Liquid
浓度
1 mg/mL
纯化类型
Protein A
保存液
PBS
内含物
no preservative
保存条件
-20° C, Avoid Freeze/Thaw Cycles
运输条件
Ambient (domestic); Wet ice (international)
RRID
AB_2536662
产品详细信息
The M810 MCP-4 antibody (clone 8C12) has successfully been paired as the coating antibody in a sandwich ELISA with detection antibody M809 (biotinylated conjugate of clone 3G4). The M810 MCP-4 antibody (biotinylated conjugate of clone 8C12) has also been used successfully in a sandwich ELISA as a detection antibody when paired with M809 (clone 3G4) as the coating antibody.
靶标信息
Monocyte Chemotactic Protein 4 (MCP-4), also called CCL13, is induced by inflammatory proteins such as IL-1 and TNFalpha. MCP-4 is a ligand for three different G protein coupled receptors, CCR2, CCR3 and CCR5. MCP-4 activates signaling in monocytes, T lymphocytes, eosinophils and basophils and this signaling is associated with the allergic response.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
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生物信息学
蛋白别名: C-C motif chemokine 13; CCL 13; chemokine (C-C motif) ligand 13; CK-beta-10; H-MCP-4; MCP-4; Monocyte chemoattractant protein 4; Monocyte chemotactic protein 4; NCC-1; new CC chemokine 1; small inducible cytokine subfamily A (Cys-Cys), member 13; Small-inducible cytokine A13
基因别名: CCL13; CKb10; MCP-4; MCP4; NCC-1; NCC1; SCYA13; SCYL1
UniProt ID: (Human) Q99616
Entrez Gene ID: (Human) 6357
monocyte chemotaxis
cellular calcium ion homeostasis
chemotaxis
inflammatory response
cytoskeleton organization
signal transduction
G-protein coupled receptor signaling pathway
cell-cell signaling
regulation of cell shape
neutrophil chemotaxis
positive regulation of GTPase activity
eosinophil chemotaxis
lymphocyte chemotaxis
positive regulation of inflammatory response
chemokine-mediated signaling pathway
positive regulation of ERK1 and ERK2 cascade
cellular response to interferon-gamma
cellular response to interleukin-1
cellular response to tumor necrosis factor
