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Invitrogen
MCP-5 Polyclonal Antibody, Biotin, PeproTech®
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MCP-5 Antibody (500-P61BT-25UG) in WB
Western Blot: To detect mMCP-5 by Western Blot analysis MCP-5 Polyclonal Antibody, Biotin (Product # 500-P61BT-500UG) can be used at a concentration of 0.1-0.2 µg/mL. Used in conjunction with compatible secondary reagents the detection limit for Recombinant mMCP-5 is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.
产品信息
500-P61BT-25UG
产品规格
种属反应
Mouse
宿主/亚型
Rabbit
分类
Polyclonal
类型
Antibody
抗原
E.coli-derived Recombinant Murine MCP-5 (CCL12)
偶联物
形式
Lyophilized
浓度
0.1-1.0 mg/mL
纯化类型
Antigen affinity chromatography
保存液
PBS
内含物
no preservative
保存条件
-20°C
运输条件
Ambient
RRID
产品详细信息
AA Sequence of recombinant protein: GPDAVSTPVT CCYNVVKQKI HVRKLKSYRR ITSSQCPREA VIFRTILDKE ICADPKEKWV KNSINHLDKT SQTFILEPSC LG.
Preparation: Produced from sera of rabbits immunized with highly pure Recombinant Murine MCP-5 (CCL12). Anti-Murine MCP-5 (CCL12)-specific antibody was purified by affinity chromatography and then biotinylated.
Sandwich ELISA: To detect mMCP-5 by sandwich ELISA (using 100 µL/well antibody solution) a concentration of 0.25-1.0 µg/mL of this antibody is required. This biotinylated polyclonal antibody, in conjunction with PeproTech Polyclonal Anti-Murine MCP-5 (500-P61) as a capture antibody, allows the detection of at least 0.2-0.4 ng/well of Recombinant mMCP-5.
Western Blot: To detect mMCP-5 by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 µg/mL. Used in conjunction with compatible secondary reagents the detection limit for Recombinant mMCP-5 is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.
500-P61BT-1MG will be provided as 2 x 500 µg
靶标信息
Chemotactic factor that attracts eosinophils, monocytes, and lymphocytes but not neutrophils. Potent monocyte active chemokine that signals through CCR2. Involved in allergic inflammation and the host response to pathogens and may play a pivotal role during early stages of allergic lung inflammation.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
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生物信息学
蛋白别名: C-C motif chemokine 12; MCP-1-related chemokine; MCP-5; Monocyte chemoattractant protein 5; Monocyte chemotactic protein 5; small inducible cytokine A12; Small-inducible cytokine A12
基因别名: Ccl12; MCP-5; Mcp5; Scya12
UniProt ID: (Mouse) Q62401
Entrez Gene ID: (Mouse) 20293
MAPK cascade
angiogenesis
monocyte chemotaxis
positive regulation of leukocyte migration
chemotaxis
inflammatory response
immune response
cytoskeleton organization
regulation of cell shape
cytokine-mediated signaling pathway
lipopolysaccharide-mediated signaling pathway
negative regulation of glial cell apoptotic process
protein kinase B signaling
negative regulation of neuron apoptotic process
astrocyte cell migration
macrophage chemotaxis
negative regulation of leukocyte proliferation
cellular response to organic cyclic compound
positive regulation of calcium ion import
negative regulation of natural killer cell chemotaxis
