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Invitrogen
MDA5 Polyclonal Antibody
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图: 1 / 12
MDA5 Antibody (PA5-89344) in ICC/IF
Immunofluorescence analysis of Interferon-induced helicase C domain-containing protein 1 was performed using 70% confluent log phase A549 IFN gamma, 100 units/mL for 24 hrs. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 2% BSA for 1 hour at room temperature. The cells were labeled with MDA5 Polyclonal Antibody (Product # PA5-89344) at 5 µg/mL in 0.1% BSA, incubated at 4 degree celsius overnight and then labeled with Goat anti-Rabbit IgG (H+L) Highly Cros... View More
Please note: We are reviewing Western blot images included in the antibody testing data in our catalog, including those provided by third parties. Unless expressly labeled or annotated as “raw-unedited”, Western blot images included in the antibody testing data in our catalog may have been edited, optimized or otherwise adjusted for presentation.
产品信息
PA5-89344
应用
建议稀释比
已发表文章
产品规格
种属反应
Human,
Mouse,
Rat
宿主/亚型
Rabbit
/ IgG
分类
Polyclonal
类型
Antibody
抗原
Recombinant protein (or fragment)
偶联物
Unconjugated
Unconjugated
Unconjugated
形式
Liquid
浓度
1.7 mg/mL
规格
170 µg
纯化类型
Affinity Chromatography
保存液
PBS, pH 7.3, with 50% glycerol
内含物
0.09% sodium azide
保存条件
-20°C, Avoid Freeze/Thaw Cycles
运输条件
Ambient (domestic); Wet ice (international)
RRID
产品详细信息
Positive Samples: THP-1; Cellular Location: Cytoplasm, Nucleus
Immunogen sequence: MSNGYSTDEN FRYLISCFRA RVKMYIQVEP VLDYLTFLPA EVKEQIQRTV ATSGNMQAVE LLLSTLEKGV WHLGWTREFV EALRRTGSPL AARYMNPELT DLPSPSFENA HDEYLQLLNL LQPTLVDKLL VRDVLDKCME EELLTIEDRN RIAAAENNGN ESGVRELLKR IVQKENWFSA FLNVLRQTGN NELVQELTGS DCSES
靶标信息
DEAD box proteins, characterized by the conserved motif Asp-Glu-Ala-Asp and a protein kinase C-activating compound, mezerein. Irreversible reprogramming of melanomas can be achieved by treatment with both these agents; treatment with either agent alone only achieves reversible differentiation.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
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生物信息学
蛋白别名: Helicard; Helicase with 2 CARD domains; Interferon induced with helicase C domain protein 1; Interferon-induced helicase C domain-containing protein 1; MDA-5; Melanoma differentiation-associated protein 5; MGC133047; RIG-I-like receptor 2; RLR-2
基因别名: 9130009C22Rik; Helicard; Hlcd; Ifih1; MDA5; RGD1560496; RLR-2
UniProt ID: (Mouse) Q8R5F7
Entrez Gene ID: (Rat) 499801, (Mouse) 71586
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