Invitrogen

MDC1 Polyclonal Antibody

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MDC1 Antibody in Immunocytochemistry (ICC/IF) — Immunofluorescence analysis of Mediator of DNA damage checkpoint protein 1 was performed using 70% confluent log phase HCT 116 Nocodazole treated (200 nmol/L for 16hrs). The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 2% BSA for 45 minutes at room temperature. The cells were labeled with MDC1 Polyclonal Antibody (Product # PA5-97022) at 1:100 in 0.1% BSA, incubated at 4 degree celsius overnight, and then labeled with Donkey anti-Rabbit IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor Plus 488 (Product # A32790), (1:2000 dilution), for 45 minutes at room temperature (Panel a: Green). Nuclei (Panel b: Blue) were stained with ProLong™ Diamond Antifade Mountant with DAPI (Product # P36962). F-actin (Panel c: Red) was stained with Rhodamine Phalloidin (Product # R415, 1:300). Panel d represents the merged image showing Nuclear foci localization. Panel e represents untreated cells with nuclear localization. Panel f represents control cells with no primary antibody to assess the background. The images were captured at 60X magnification.

MDC1 Antibody in Immunohistochemistry (IHC) — Immunohistochemistry analysis of MDC1 in paraffin-embedded human prostate cancer using MDC1 Polyclonal Antibody (Product # PA5-97022) at a dilution of 1:100.

MDC1 Antibody in Western Blot (WB) — Western Blot analysis of MDC1 in extracts of various cell lines using MDC1 Polyclonal Antibody (Product # PA5-97022) at a dilution of 1:1000. A HRP Goat Anti-Rabbit IgG (H+L) secondary antibody was used at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST.

产品信息

PA5-97022

应用	建议稀释比	已发表文章
免疫印迹 (WB)	1:500-1:2,000	查看1篇已发表文章 1篇已发表文章
免疫组化（石蜡） (IHC (P))	1:100 - 1:200	-
免疫细胞化学 (ICC/IF)	1:50-1:200	-

产品规格
种属反应	Human
已发表种属	Human
宿主/亚型	Rabbit / IgG
分类	Polyclonal
类型	Antibody
抗原	Recombinant fusion protein containing a sequence corresponding to amino acids 1-350 of human MDC1.
偶联物	Unconjugated
形式	Liquid
浓度	0.92 mg/mL
纯化类型	Antigen affinity chromatography
保存液	PBS, pH 7.3, with 50% glycerol
内含物	0.02% sodium azide
保存条件	-20° C, Avoid Freeze/Thaw Cycles
RRID	AB_2808824

产品详细信息

Immunogen sequence: MEDTQAIDWD VEEEEETEQS SESLRCNVEP VGRLHIFSGA HGPEKDFPLH LGKNVVGRMP DCSVALPFPS ISKQHAEIEI LAWDKAPILR DCGSLNGTQI LRPPKVLSPG VSHRLRDQEL ILFADLLCQY HRLDVSLPFV SRGPLTVEET PRVQGETQPQ RLLLAEDSEE EVDFLSERRM VKKSRTTSSS VIVPESDEEG HSPVLGGLGP PFAFNLNSDT DVEEGQQPAT EEASSAARRG ATVEAKQSEA EVVTEIQLEK DQPLVKERDN DTKVKRGAGN GVVPAGVILE RSQPPGEDSD TDVDDDSRPP GRPAEVHLER AQPFGFIDSD TDAEEERIPA TPVVIPMKKR; Positive Samples: Jurkat, LO2; Cellular Location: Chromosome, Nucleus

靶标信息

MDC1 participates in the early response to DNA damage. It is thought to be a member of the BRCT (BRCA1 C-terminus) super family of nuclear proteins. It contains an N-terminus forkhead-associated (FHA) motif, two C-terminus BRCT motifs and 13 internal repetitions of a 41 amino acid sequence.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

Product References

O-GlcNAcylation Affects the Pathway Choice of DNA Double-Strand Break Repair.

International journal of molecular sciences

Averbek S,Jakob B,Durante M,Averbeck NB

PA5-97022 was used in Western Blot to study the role of the post-translational modification, O-linked β-N-acetylglucosaminylation, on the cellular DNA damage response.

Thu May 27 00:00:00 EDT 2021

生物信息学

蛋白别名: DAAP-285E11.7; DKFZp781A0122; homologue to Drosophila photoreceptor protein calphotin; Mediator of DNA damage checkpoint protein 1; mediator of DNA-damage checkpoint 1; MGC166888; Nuclear factor with BRCT domains 1

基因别名: KIAA0170; MDC1; NFBD1

UniProt ID: (Human) Q14676

Entrez Gene ID: (Human) 9656

功能

double-strand break repair via nonhomologous end joining protein sumoylation intra-S DNA damage checkpoint

参与通路

protein binding protein C-terminus binding FHA domain binding

MDC1 Polyclonal Antibody

产品信息

PA5-97022

免疫印迹 (WB)

免疫组化（石蜡） (IHC (P))

免疫细胞化学 (ICC/IF)

种属反应

已发表种属

宿主/亚型

分类

类型

抗原

偶联物

形式

浓度

纯化类型

保存液

内含物

保存条件

RRID

产品详细信息

靶标信息

生物信息学

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