Invitrogen

MDC1 Polyclonal Antibody

Product Image Gallery

MDC1 Antibody in Immunocytochemistry (ICC/IF) — Immunofluorescence analysis of Mediator of DNA damage checkpoint protein 1 was performed using 70% confluent log phase HCT 116 Nocodazole treated (200 nmol/L for 16hrs). The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 2% BSA for 45 minutes at room temperature. The cells were labeled with MDC1 Polyclonal Antibody (Product # PA5-97022) at 1:100 in 0.1% BSA, incubated at 4 degree celsius overnight, and then labeled with Donkey anti-Rabbit IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor Plus 488 (Product # A32790), (1:2000 dilution), for 45 minutes at room temperature (Panel a: Green). Nuclei (Panel b: Blue) were stained with ProLong™ Diamond Antifade Mountant with DAPI (Product # P36962). F-actin (Panel c: Red) was stained with Rhodamine Phalloidin (Product # R415, 1:300). Panel d represents the merged image showing Nuclear foci localization. Panel e represents untreated cells with nuclear localization. Panel f represents control cells with no primary antibody to assess the background. The images were captured at 60X magnification.

MDC1 Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry analysis of MDC1 in paraffin-embedded human prostate cancer using MDC1 Polyclonal Antibody (Product # PA5-97022) at a dilution of 1:100.

MDC1 Antibody in Western Blot (WB) — Western Blot analysis of MDC1 in extracts of various cell lines using MDC1 Polyclonal Antibody (Product # PA5-97022) at a dilution of 1:1000. A HRP Goat Anti-Rabbit IgG (H+L) secondary antibody was used at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST.

产品信息

PA5-97022

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1:500-1:2,000

查看1篇已发表文章 1篇已发表文章

免疫组化（石蜡） (IHC (P))

1:100 - 1:200

免疫细胞化学 (ICC/IF)

1:50-1:200

产品规格

种属反应

Human

已发表种属

Human

宿主/亚型

Rabbit / IgG

分类

Polyclonal

类型

Antibody

抗原

Recombinant fusion protein containing a sequence corresponding to amino acids 1-350 of human MDC1.

查看免疫原

偶联物

Unconjugated

形式

Liquid

浓度

0.28 mg/mL

纯化类型

Affinity Chromatography

保存液

PBS, pH 7.3, with 50% glycerol

内含物

0.02% sodium azide

保存条件

-20° C, Avoid Freeze/Thaw Cycles

RRID

AB_2808824

产品详细信息

Immunogen sequence: MEDTQAIDWD VEEEEETEQS SESLRCNVEP VGRLHIFSGA HGPEKDFPLH LGKNVVGRMP DCSVALPFPS ISKQHAEIEI LAWDKAPILR DCGSLNGTQI LRPPKVLSPG VSHRLRDQEL ILFADLLCQY HRLDVSLPFV SRGPLTVEET PRVQGETQPQ RLLLAEDSEE EVDFLSERRM VKKSRTTSSS VIVPESDEEG HSPVLGGLGP PFAFNLNSDT DVEEGQQPAT EEASSAARRG ATVEAKQSEA EVVTEIQLEK DQPLVKERDN DTKVKRGAGN GVVPAGVILE RSQPPGEDSD TDVDDDSRPP GRPAEVHLER AQPFGFIDSD TDAEEERIPA TPVVIPMKKR; Positive Samples: Jurkat, LO2; Cellular Location: Chromosome, Nucleus

靶标信息

MDC1 participates in the early response to DNA damage. It is thought to be a member of the BRCT (BRCA1 C-terminus) super family of nuclear proteins. It contains an N-terminus forkhead-associated (FHA) motif, two C-terminus BRCT motifs and 13 internal repetitions of a 41 amino acid sequence.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

Product References

O-GlcNAcylation Affects the Pathway Choice of DNA Double-Strand Break Repair.

International journal of molecular sciences

Averbek S,Jakob B,Durante M,Averbeck NB

PA5-97022 was used in Western Blot to study the role of the post-translational modification, O-linked β-N-acetylglucosaminylation, on the cellular DNA damage response.

Thu May 27 00:00:00 EDT 2021

生物信息学

蛋白别名: DAAP-285E11.7; DKFZp781A0122; homologue to Drosophila photoreceptor protein calphotin; Mediator of DNA damage checkpoint protein 1; mediator of DNA-damage checkpoint 1; MGC166888; Nuclear factor with BRCT domains 1

基因别名: KIAA0170; MDC1; NFBD1

UniProt ID: (Human) Q14676

Entrez Gene ID: (Human) 9656

功能

double-strand break repair via nonhomologous end joining protein sumoylation intra-S DNA damage checkpoint

参与通路

protein binding protein C-terminus binding FHA domain binding