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Invitrogen
MED4 Polyclonal Antibody
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MED4 Antibody (PA5-97188) in WB
Western blot analysis of MED4 in extracts of various cell lines. Samples were incubated with MED4 Polyclonal antibody (Product # PA5-97188) using a dilution of 1:1,000, followed by HRP Goat Anti-Rabbit IgG (H+L) at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.
Please note: We are reviewing Western blot images included in the antibody testing data in our catalog, including those provided by third parties. Unless expressly labeled or annotated as “raw-unedited”, Western blot images included in the antibody testing data in our catalog may have been edited, optimized or otherwise adjusted for presentation.
产品信息
PA5-97188
产品规格
种属反应
Human
宿主/亚型
Rabbit
/ IgG
分类
Polyclonal
类型
Antibody
抗原
Recombinant fusion protein containing a sequence corresponding to amino acids 1-270 of human MED4 (NP_0548851)
偶联物
Unconjugated
Unconjugated
Unconjugated
形式
Liquid
浓度
1.03 mg/mL
规格
103 µg
纯化类型
Affinity Chromatography
保存液
PBS, pH 7.3, with 50% glycerol
内含物
0.02% sodium azide
保存条件
-20°C, Avoid Freeze/Thaw Cycles
运输条件
Ambient (domestic); Wet ice (international)
RRID
产品详细信息
Immunogen sequence: MAASSSGEKE KERLGGGLGV AGGNSTRERL LSALEDLEVL SRELIEMLAI SRNQKLLQAG EENQVLELLI HRDGEFQELM KLALNQGKIH HEMQVLEKEV EKRDSDIQQL QKQLKEAEQI LATAVYQAKE KLKSIEKARK GAISSEEIIK YAHRISASNA VCAPLTWVPG DPRRPYPTDL EMRSGLLGQM NNPSTNGVNG HLPGDALAAG RLPDVLAPQY PWQSNDMSMN MLPPNHSSDF LLEPPGHNKE NEDDVEIMST DSSSSSSESD; Positive Samples: Jurkat, SH-SY5Y, HL-60, SW480, HT-1080; Cellular Location: Nucleus
靶标信息
The Mediator is a multiprotein coactivator that is required by DNA-binding transcription factors for activation of polymerase II transcribed genes. MED4 appears to be a core Mediator subunit and is found in nearly all Mediator preparations (Sato et al., 2004).
仅用于科研。不用于诊断过程。未经明确授权不得转售。
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生物信息学
蛋白别名: activator-recruited cofactor 36 kDa component; FLJ10956; mediator, 34-kD subunit, homolog; RP11-90M2.2; unnamed protein product; VDRIP; vitamin D receptor-interacting protein, 36-kD; vitamin D3 receptor-interacting protein complex 36 kDa component
基因别名: ARC36; DRIP36; HSPC126; TRAP36; VDRIP
UniProt ID: (Human) Q9NPJ6
Entrez Gene ID: (Human) 29079
regulation of transcription from RNA polymerase II promoter
transcription from RNA polymerase II promoter
positive regulation of transcription elongation from RNA polymerase II promoter
positive regulation of transcription, DNA-templated
RNA polymerase II transcriptional preinitiation complex assembly
positive regulation of transcription initiation from RNA polymerase II promoter
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