Invitrogen

MEF2D Polyclonal Antibody

Name: MEF2D Polyclonal Antibody
Brand: Invitrogen
SKU: PA5-30950

高级验证

查看其他21个MEF2D抗体

说明书

实验方案

问题与答案

技术支持

说明书

实验方案

问题与答案

技术支持

说明书

实验方案

问题与答案

技术支持

抗体检测数据 (3)
高级验证 (1)

MEF2D Antibody in Immunocytochemistry (ICC/IF)

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MEF2D Antibody (PA5-30950) in ICC/IF

Immunofluorescence analysis of MEF2D was performed using HeLa cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 1% BSA for 1 hour at room temperature. The cells were labeled with MEF2D Polyclonal Antibody (Product # PA5-30950) at a dilution of 1:50 in 0.1% BSA, incubated at 4 degree Celsius overnight and then labeled with Goat anti-Rabbit IgG (Heavy Chain) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate (Product # A27034) at a dilution of ... View More

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MEF2D Antibody — Relative expression of proteins demonstrates antibody specificity. Western blot of MEF2D using MEF2D Polyclonal Antibody (Product # PA5-30950), shows increased expression of MEF2D in differentiated C2C12 cells compared to undifferentiated C2C12. {RE}

产品信息

PA5-30950

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1:500-1:3,000

免疫细胞化学 (ICC/IF)

1:50

产品规格

种属反应

Human, Mouse, Rat

宿主/亚型

Rabbit / IgG

分类

Polyclonal

类型

Antibody

抗原

Recombinant protein encompassing a sequence within the center region of human MEF2D. The exact sequence is proprietary.

偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

0.85 mg/mL

纯化类型

Antigen affinity chromatography

保存液

0.1M tris glycine, pH 7, with 20% glycerol

内含物

0.01% thimerosal

保存条件

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

运输条件

Ambient (domestic); Wet ice (international)

AB_2548424

产品详细信息

Recommended positive controls: 293T, A431, Jurkat, Raji.

Predicted reactivity: Mouse (98%), Rat (95%), Xenopus laevis (83%), Chicken (84%), Bovine (94%).

Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.

靶标信息

This gene is a member of the myocyte-specific enhancer factor 2 (MEF2) family of transcription factors. Members of this family are involved in control of muscle and neuronal cell differentiation and development, and are regulated by class II histone deacetylases. Fusions of the encoded protein with Deleted in Azoospermia-Associated Protein 1 (DAZAP1) due to a translocation have been found in an acute lymphoblastic leukemia cell line, suggesting a role in leukemogenesis. The encoded protein may also be involved in Parkinson disease and myotonic dystrophy. Alternative splicing results in multiple transcript variants.

⚠WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause birth defects or other reproductive harm. For more information go to www.P65Warnings.ca.gov.

仅用于科研。不用于诊断过程。未经明确授权不得转售。