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Invitrogen
MERTK Monoclonal Antibody (A3KCAT), eBioscience™
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产品信息
14-9053-82
产品规格
种属反应
Human
宿主/亚型
Mouse
/ IgG1, kappa
分类
Monoclonal
类型
Antibody
克隆号
A3KCAT
抗原
Full extracellular domain of human MerTK (N-terminal part)
偶联物
形式
Liquid
浓度
0.5 mg/mL
纯化类型
Affinity chromatography
保存液
PBS
内含物
0.09% sodium azide
保存条件
4°C, store in dark, DO NOT FREEZE!
运输条件
Ambient (domestic); Wet ice (international)
RRID
产品详细信息
This A3KCAT antibody recognizes human MerTK, known also as MER or c-mer, a 170-210 kDa member of the TAM family of tyrosine kinase receptors that also includes Axl and Tyro3. MerTK is expressed on a subset of anti-inflammatory macrophages and is involved in the removal of apoptotic cells. This process relies on two soluble ligands of MerTK, Protein S and Gas6, that coat the surface of cells undergoing apoptosis. Upon binding these ligands, MerTK undergoes autophosphorylation at multiple tyrosine residues that activate the PI3K and Akt pathways. This results in the phagocytosis of target cells and also the direct inhibition of TLR-induced production of pro-inflammatory cytokines by the phagocytes. MerTK may also function as a putative entry receptor for filoviruses. Deficiency of MerTK causes general autoimmunity, inflammation, and accumulation of apoptotic bodies. MerTK can be released from the cell surface by metalloproteinases, and this process is significantly enhanced when macrophages are stimulated e.g. with LPS. MerTK is often expressed on malignant cells, and may be implicated in immune evasion.
This A3KCAT antibody has been tested by immunohistochemistry of formalin-fixed paraffin embedded tissue using low or high pH antigen retrieval and can be used at less than or equal to 0.5 µg/mL. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.
Purity: Greater than 90%, as determined by SDS-PAGE.
Aggregation: Less than 10%, as determined by HPLC.
Filtration: 0.2 µm post-manufacturing filtered.
靶标信息
MERTK (cMER) is a tyrosine kinase proto-oncogene and is involved in the retinal pigment epithelium (RPE) phagocytosis pathway, which is implicated in human retinal disease. Regulates many physiological processes including cell survival, migration, differentiation, and phagocytosis of apoptotic cells (efferocytosis). Ligand binding at the cell surface induces autophosphorylation of MERTK on its intracellular domain that provides docking sites for downstream signaling molecules. Following activation by ligand, interacts with GRB2 or PLCG2 and induces phosphorylation of MAPK1, MAPK2, FAK/PTK2 or RAC1. MERTK signaling plays a role in various processes such as macrophage clearance of apoptotic cells, platelet aggregation, cytoskeleton reorganization and engulfment. Functions in the retinal pigment epithelium (RPE) as a regulator of rod outer segments fragments phagocytosis. Plays also an important role in inhibition of Toll-like receptors (TLRs)-mediated innate immune response by activating STAT1, which selectively induces production of suppressors of cytokine signaling SOCS1 and SOCS3.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
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生物信息学
蛋白别名: c-mer proto-oncogene tyrosine kinase; MER receptor tyrosine kinase; MGC133349; Proto-oncogene c-Mer; Receptor tyrosine kinase MerTK; sMER; sMERTK; soluble MER; soluble MERTK; STK kinase; Tyrosine-protein kinase Mer
基因别名: c-Eyk; c-mer; MER; MERTK; RP38; Tyro12
UniProt ID: (Human) Q12866
Entrez Gene ID: (Human) 10461
natural killer cell differentiation
protein phosphorylation
phagocytosis
cell surface receptor signaling pathway
cell-cell signaling
spermatogenesis
peptidyl-tyrosine phosphorylation
platelet activation
secretion by cell
substrate adhesion-dependent cell spreading
apoptotic cell clearance
protein kinase B signaling
positive regulation of phagocytosis
leukocyte migration
negative regulation of lymphocyte activation
retina development in camera-type eye
vagina development
negative regulation of leukocyte apoptotic process
