Invitrogen

METT10D Polyclonal Antibody

查看其他8个METT10D抗体

问题与答案

问题与答案

问题与答案

抗体检测数据 (6)

METT10D Antibody in Immunocytochemistry (ICC/IF)

METT10D Antibody in Immunocytochemistry (ICC/IF)

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METT10D Antibody (PA5-120402) in ICC/IF

Immunofluorescence analysis of METT10D in NIH/3T3 cells. Samples were incubated with METT10D Polyclonal antibody (Product # PA5-120402) using a dilution of 1:100. Blue: DAPI for nuclear staining.

METT10D Antibody in Immunocytochemistry (ICC/IF)

METT10D Antibody in Immunocytochemistry (ICC/IF)

METT10D Antibody in Immunocytochemistry (ICC/IF)

METT10D Antibody in Immunohistochemistry (Paraffin) (IHC (P))

METT10D Antibody in Western Blot (WB)

METT10D Antibody in Immunoprecipitation (IP)

Product Image Gallery

METT10D Antibody in Immunocytochemistry (ICC/IF) — Immunofluorescence analysis of METT10D in NIH/3T3 cells. Samples were incubated with METT10D Polyclonal antibody (Product # PA5-120402) using a dilution of 1:100. Blue: DAPI for nuclear staining.

METT10D Antibody in Immunocytochemistry (ICC/IF) — Immunofluorescence analysis of METT10D in U-2 OS cells. Samples were incubated with METT10D Polyclonal antibody (Product # PA5-120402) using a dilution of 1:100. Blue: DAPI for nuclear staining.

METT10D Antibody in Immunocytochemistry (ICC/IF) — Immunofluorescence analysis of METT10D in C6 cells. Samples were incubated with METT10D Polyclonal antibody (Product # PA5-120402) using a dilution of 1:100. Blue: DAPI for nuclear staining.

METT10D Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry analysis of METT10D in paraffin-embedded human spleen. Samples were incubated with METT10D Polyclonal antibody (Product # PA5-120402) using a dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

METT10D Antibody in Western Blot (WB) — Western blot analysis of METT10D in various lysates. Samples were incubated with METT10D Polyclonal antibody (Product # PA5-120402) using a dilution of 1:1,000, followed by HRP Goat Anti-Rabbit IgG (H+L) at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 60s.

METT10D Antibody in Immunoprecipitation (IP) — Immunoprecipitation of METT10D in 300 μg extracts of LNCaP cells. Samples were precipitated with 3 μg METT10D Polyclonal antibody (Product # PA5-120402). Western blot was performed from the immunoprecipitate using METT10D Polyclonal antibody (Product # PA5-120402) at a dilution of 1:1,000.

Immunogen image

METT10D Polyclonal Antibody

Immunogen Image METT10D Polyclonal Antibody

产品信息

PA5-120402

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1:500-1:1,000

-

免疫组化（石蜡） (IHC (P))

1:50-1:200

-

免疫细胞化学 (ICC/IF)

1:50-1:200

-

酶联免疫吸附实验 (ELISA)

1 µg/mL

-

免疫沉淀 (IP)

0.5 µg-4 µg antibody for 200 µg-400 µg extracts of

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产品规格

种属反应

Human, Mouse, Rat

宿主/亚型

Rabbit / IgG

分类

Polyclonal

类型

Antibody

抗原

Recombinant fusion protein containing a sequence corresponding to amino acids 263-562 of human METTL16 (NP_076991.3).

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偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

1.4 mg/mL

纯化类型

Affinity Chromatography

保存液

PBS, pH 7.3, with 50% glycerol

内含物

0.09% sodium azide

保存条件

-20°C, Avoid Freeze/Thaw Cycles

运输条件

Ambient (domestic); Wet ice (international)

产品详细信息

Positive test controls include: HeLa, A-549, NIH/3T3, C6, LNCaP, Rat testis.

Immunogen sequence: RIQGVPKVTY TEFCQGRTMR WALAWSFYDD VTVPSPPSKR RKLEKPRKPI TFVVLASVMK ELSLKASPLR SETAEGIVVV TTWIEKILTD LKVQHKRVPC GKEEVSLFLT AIENSWIHLR RKKRERVRQL REVPRAPEDV IQALEEKKPT PKESGNSQEL ARGPQERTPC GPALREGEAA AVEGPCPSQE SLSQEENPEP TEDERSEEKG GVEVLESCQG SSNGAQDQEA SEQFGSPVAE RGKRLPGVAG QYLFKCLINV KKEVDDALVE MHWVEGQNRD LMNQLCTYIR NQIFRLVAVN

靶标信息

METT10D is a probable methyltransferase.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

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引用该产品

生物信息学

蛋白别名: methyltransferase 10 domain containing; Methyltransferase 10 domain-containing protein; Methyltransferase-like protein 16; putative methyltransferase METT10D; RNA N(6)-adenosine-methyltransferase METTL16; U6 small nuclear RNA (adenine-(43)-N(6))-methyltransferase

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基因别名: 2610100D03Rik; 2810013M15Rik; A830095F14Rik; AI846133; METT10D; METTL16

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UniProt ID: (Human) Q86W50, (Mouse) Q9CQG2

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Entrez Gene ID: (Human) 79066, (Mouse) 67493

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poly(A) RNA binding 23S rRNA (adenine(1618)-N(6))-methyltransferase activity rRNA (adenine-N6,N6-)-dimethyltransferase activity protein C-terminal carboxyl O-methyltransferase activity tRNA (guanine-N2-)-methyltransferase activity methyltransferase activity C-methyltransferase activity N-methyltransferase activity O-methyltransferase activity S-methyltransferase activity RNA methyltransferase activity mRNA methyltransferase activity tRNA methyltransferase activity protein methyltransferase activity 2-polyprenyl-6-methoxy-1,4-benzoquinone methyltransferase activity rRNA methyltransferase activity rRNA (uridine-2'-O-)-methyltransferase activity rRNA (adenine-N6-)-methyltransferase activity DNA-methyltransferase activity tRNA (guanine-N1-)-methyltransferase activity rRNA (cytosine-C5-)-methyltransferase activity selenocysteine methyltransferase activity arginine N-methyltransferase activity protein-arginine N-methyltransferase activity lysine N-methyltransferase activity protein-lysine N-methyltransferase activity tRNA (uracil) methyltransferase activity tRNA (guanine) methyltransferase activity tRNA (adenine) methyltransferase activity tRNA (cytosine) methyltransferase activity tRNA (cytosine-5-)-methyltransferase activity tRNA (adenine-N1-)-methyltransferase activity rRNA (adenine) methyltransferase activity rRNA (cytosine) methyltransferase activity rRNA (guanine) methyltransferase activity rRNA (uridine) methyltransferase activity transferase activity protein C-terminal leucine carboxyl O-methyltransferase activity 1-phenanthrol methyltransferase activity protein-arginine N5-methyltransferase activity methylarsonite methyltransferase activity dimethylarsinite methyltransferase activity 4,5-dihydroxybenzo(a)pyrene methyltransferase activity 1-hydroxypyrene methyltransferase activity 1-hydroxy-6-methoxypyrene methyltransferase activity demethylmenaquinone methyltransferase activity cobalt-precorrin-6B C5-methyltransferase activity cobalt-precorrin-7 C15-methyltransferase activity cobalt-precorrin-5B C1-methyltransferase activity cobalt-precorrin-3 C17-methyltransferase activity dimethylamine methyltransferase activity hydroxyneurosporene-O-methyltransferase activity tRNA (adenine-57, 58-N(1)-) methyltransferase activity methylamine-specific methylcobalamin:coenzyme M methyltransferase activity trimethylamine methyltransferase activity methanol-specific methylcobalamin:coenzyme M methyltransferase activity monomethylamine methyltransferase activity P-methyltransferase activity Se-methyltransferase activity 2-phytyl-1,4-naphthoquinone methyltransferase activity tRNA (uracil-2'-O-)-methyltransferase activity tRNA (cytosine-2'-O-)-methyltransferase activity phosphomethylethanolamine N-methyltransferase activity tRNA (cytosine-3-)-methyltransferase activity rRNA (cytosine-2'-O-)-methyltransferase activity rRNA (cytosine-N4-)-methyltransferase activity trihydroxyferuloyl spermidine O-methyltransferase activity methyltransferase

rRNA base methylation biological_process methylation