Invitrogen

METTL23 Polyclonal Antibody

查看其他3个METTL23抗体

抗体检测数据 (1)

METTL23 Antibody in Western Blot (WB)

METTL23 Antibody in Western Blot (WB)

图: 1 / 1

METTL23 Antibody (PA5-144081) in WB

Western blot analysis of METTL23 using METTL23 antibody (Product # PA5-144081). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 µg of sample under reducing conditions. Lane 1: human HCT whole cell lysates; Lane 2: human Caco-2 whole cell lysates; Lane 3: human SHY whole cell lysates; Lane 4: human U87 whole cell lysates; Lane 5: mouse GL261 whole cell lysates. After Electrophoresis, proteins were transferred to a PVDF membrane a... View More

METTL23 Antibody in Western Blot (WB)

Product Image Gallery

METTL23 Antibody in Western Blot (WB) — Western blot analysis of METTL23 using METTL23 antibody (Product # PA5-144081). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 µg of sample under reducing conditions. Lane 1: human HCT whole cell lysates; Lane 2: human Caco-2 whole cell lysates; Lane 3: human SHY whole cell lysates; Lane 4: human U87 whole cell lysates; Lane 5: mouse GL261 whole cell lysates. After Electrophoresis, proteins were transferred to a PVDF membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-METTL23 antigen affinity purified polyclonal antibody (Product # PA5-144081) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0. 1%Tween 3 times with 5 minutes each and detected by Licor IR800 rabbit antibody at a dilution of 1:5,000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system.

产品信息

PA5-144081

应用

建议稀释比

已发表文章

免疫印迹 (WB)

0.5 µg/mL

-

酶联免疫吸附实验 (ELISA)

Assay-dependent

-

产品规格

种属反应

Human, Mouse

宿主/亚型

Rabbit / IgG

分类

Polyclonal

类型

Antibody

抗原

Human METTL23 between 11-190

查看免疫原

偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

500 µg/mL

纯化类型

Antigen affinity chromatography

保存液

PBS with 50% glycerol

内含物

0.02% sodium azide

保存条件

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

运输条件

Wet ice

RRID

AB_3075295

靶标信息

The protein encoded by this gene functions as a transcription factor regulator in the transcriptional pathway for human cognition. It is a partner of the alpha subunit of the GA-binding protein transcription factor. Mutations in this gene cause mild autosomal recessive intellectual disability. Alternative splicing results in multiple transcript variants.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

篇参考文献 (0)

您是否在文献中引用过该产品？请点击下方按钮邮件告知我们。

引用该产品

生物信息学

蛋白别名: Histone-arginine methyltransferase METTL23; Methyltransferase-like protein 23

查看更多收起

基因别名: 1110005A03Rik; 1500035B17Rik; 4933424L15Rik; C17orf95; METTL23; MRT44

查看更多收起

UniProt ID: (Human) Q86XA0, (Mouse) A2AA28

查看更多收起

Entrez Gene ID: (Human) 124512, (Mouse) 74319

查看更多收起

methyltransferase activity rRNA (adenine-N6,N6-)-dimethyltransferase activity molecular_function protein C-terminal carboxyl O-methyltransferase activity tRNA (guanine-N2-)-methyltransferase activity C-methyltransferase activity N-methyltransferase activity O-methyltransferase activity S-methyltransferase activity RNA methyltransferase activity mRNA methyltransferase activity tRNA methyltransferase activity protein methyltransferase activity 2-polyprenyl-6-methoxy-1,4-benzoquinone methyltransferase activity rRNA methyltransferase activity rRNA (uridine-2'-O-)-methyltransferase activity rRNA (adenine-N6-)-methyltransferase activity DNA-methyltransferase activity tRNA (guanine-N1-)-methyltransferase activity rRNA (cytosine-C5-)-methyltransferase activity selenocysteine methyltransferase activity arginine N-methyltransferase activity protein-arginine N-methyltransferase activity lysine N-methyltransferase activity protein-lysine N-methyltransferase activity tRNA (uracil) methyltransferase activity tRNA (guanine) methyltransferase activity tRNA (adenine) methyltransferase activity tRNA (cytosine) methyltransferase activity tRNA (cytosine-5-)-methyltransferase activity tRNA (adenine-N1-)-methyltransferase activity rRNA (adenine) methyltransferase activity rRNA (cytosine) methyltransferase activity rRNA (guanine) methyltransferase activity rRNA (uridine) methyltransferase activity transferase activity protein C-terminal leucine carboxyl O-methyltransferase activity 1-phenanthrol methyltransferase activity protein-arginine N5-methyltransferase activity methylarsonite methyltransferase activity dimethylarsinite methyltransferase activity 4,5-dihydroxybenzo(a)pyrene methyltransferase activity 1-hydroxypyrene methyltransferase activity 1-hydroxy-6-methoxypyrene methyltransferase activity demethylmenaquinone methyltransferase activity cobalt-precorrin-6B C5-methyltransferase activity cobalt-precorrin-7 C15-methyltransferase activity cobalt-precorrin-5B C1-methyltransferase activity cobalt-precorrin-3 C17-methyltransferase activity dimethylamine methyltransferase activity hydroxyneurosporene-O-methyltransferase activity tRNA (adenine-57, 58-N(1)-) methyltransferase activity methylamine-specific methylcobalamin:coenzyme M methyltransferase activity trimethylamine methyltransferase activity methanol-specific methylcobalamin:coenzyme M methyltransferase activity monomethylamine methyltransferase activity P-methyltransferase activity Se-methyltransferase activity 2-phytyl-1,4-naphthoquinone methyltransferase activity tRNA (uracil-2'-O-)-methyltransferase activity tRNA (cytosine-2'-O-)-methyltransferase activity phosphomethylethanolamine N-methyltransferase activity tRNA (cytosine-3-)-methyltransferase activity rRNA (cytosine-2'-O-)-methyltransferase activity rRNA (cytosine-N4-)-methyltransferase activity trihydroxyferuloyl spermidine O-methyltransferase activity

参与通路

biological_process methylation