Invitrogen

METTL23 Polyclonal Antibody

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METTL23 Antibody in Western Blot (WB)

METTL23 Antibody in Western Blot (WB)

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METTL23 Antibody (PA5-71814) in WB

Western blot analysis of METTL23 in NCI-H460 cell line lysates. Samples were incubated with METTL23 polyclonal antibody (Product # PA5-71814). Lysates: 35 µg/lane. C17orf95 protein (arrow).

METTL23 Antibody in Western Blot (WB)

METTL23 Antibody in Western Blot, Immunocytochemistry (WB, ICC/IF)

METTL23 Antibody in Western Blot, Immunohistochemistry (WB, IHC)

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METTL23 Antibody in Western Blot (WB) — Western blot analysis of METTL23 in NCI-H460 cell line lysates. Samples were incubated with METTL23 polyclonal antibody (Product # PA5-71814). Lysates: 35 µg/lane. C17orf95 protein (arrow).

METTL23 Antibody in Western Blot, Immunocytochemistry (WB, ICC/IF) — The METTL23 c.A83G mutation leads to aberrant expression and trafficking in vitro. ( A ) METTL23 expression was reduced by the c.A83G mutation. Expression of full-length METTL23, splicing 1 (skip exon 2) and splicing 2 (skip exons 2 and 3) with the C-terminal FLAG-tag in HEK293T, COS-7, and 661W cells was determined by WB. ( B ) Quantification of METTL23 expression. Data are representative of 3 independent experiments. ( C and D ) Localization of METTL23 and different splicing products with the C-terminal FLAG-tag in transfected COS-7 ( C ) and 661W ( D ) cells by immunofluorescence. METTL23 was labeled with anti-METTL23 (green) and anti-FLAG (red) antibodies. Nuclei were stained with DAPI (blue). Scale bars: 10 mum. ( E ) 2.5D expression pattern of variant METTL23 in COS-7 and 661W cells. ( F ) Medium optical density (MOD) statistical results for METTL23 in transfected COS-7 and 661W cells. Results are representative of 4-11 independent experiments. All data are presented as the mean +- SEM. * P < 0.05, ** P < 0.01, *** P < 0.001, and **** P < 0.0001, by 1-way ANOVA followed by Tukey's multiple-comparison test.

METTL23 Antibody in Western Blot, Immunohistochemistry (WB, IHC) — The Mettl23 mutation reduces expression in vivo. ( A ) Localization of METTL23 in cynomolgus macaque retina. METTL23 was specifically expressed in RGC nuclei and optic nerve fibers, as demonstrated by immunofluorescence. Nuclei were stained with DAPI (blue) in the same section. The merged image reveals nuclear colocalization of METTL23. METTL23 expression was more intense in the central retina than in the periphery. ( B ) WT murine retina shows cytoplasmic and nuclear staining in RGCs with anti-METTL23 antibody (green) by immunofluorescence. In Mettl23 G/G and Mettl23 -/- mice, METTL23 lost specific RGC binding. ( C and D ) Higher-magnification images show a high degree of colocalization of METTL23 and RGCs in macaque ( C , higher-magnification view of GCL in A ) and murine ( D ) retinal tissue. ( E ) Mettl23 G/G and Mettl23 -/- mice lost retinal METTL23 expression as shown by WB. Scale bars: 20 mum. INL, inner nuclear layer; OPL, outer plexiform layer; ONL, outer nuclear layer; PL, photoreceptor layer.

产品信息

PA5-71814

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1:1,000

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免疫组化 (IHC)

-

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免疫组化（石蜡） (IHC (P))

-

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免疫细胞化学 (ICC/IF)

-

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产品规格

种属反应

Human

已发表种属

Human, Mouse, Non-human primate

宿主/亚型

Rabbit / IgG

分类

Polyclonal

类型

Antibody

抗原

KLH conjugated synthetic peptide between 138-166 amino acids from the C-terminal region of human C17orf95

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偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

0.5 mg/mL

纯化类型

Protein A, Antigen affinity chromatography

保存液

PBS, pH 7.4

内含物

0.09% sodium azide

保存条件

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

运输条件

Ambient (domestic); Wet ice (international)

RRID

AB_2717668

靶标信息

The protein encoded by this gene functions as a transcription factor regulator in the transcriptional pathway for human cognition. It is a partner of the alpha subunit of the GA-binding protein transcription factor. Mutations in this gene cause mild autosomal recessive intellectual disability. Alternative splicing results in multiple transcript variants.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

Product References

METTL23 mutation alters histone H3R17 methylation in normal-tension glaucoma.

The Journal of clinical investigation

Pan Y,Suga A,Kimura I,Kimura C,Minegishi Y,Nakayama M,Yoshitake K,Iejima D,Minematsu N,Yamamoto M,Mabuchi F,Takamoto M,Shiga Y,Araie M,Kashiwagi K,Aihara M,Nakazawa T,Iwata T

PA5-71814 was used in Western Blot, Immunohistochemistry (Paraffin) to suggest an etiologic role of METTL23 in NTG with tissue-specific pathology.

Tue Nov 01 00:00:00 UTC 2022

METTL23 mutation alters histone H3R17 methylation in normal-tension glaucoma.

The Journal of clinical investigation

Pan Y,Suga A,Kimura I,Kimura C,Minegishi Y,Nakayama M,Yoshitake K,Iejima D,Minematsu N,Yamamoto M,Mabuchi F,Takamoto M,Shiga Y,Araie M,Kashiwagi K,Aihara M,Nakazawa T,Iwata T

PA5-71814 was used in Western Blot, Immunohistochemistry (Paraffin) to suggest an etiologic role of METTL23 in NTG with tissue-specific pathology.

Tue Nov 01 00:00:00 UTC 2022

METTL23 mutation alters histone H3R17 methylation in normal-tension glaucoma.

The Journal of clinical investigation

Pan Y,Suga A,Kimura I,Kimura C,Minegishi Y,Nakayama M,Yoshitake K,Iejima D,Minematsu N,Yamamoto M,Mabuchi F,Takamoto M,Shiga Y,Araie M,Kashiwagi K,Aihara M,Nakazawa T,Iwata T

PA5-71814 was used in Western Blot, Immunohistochemistry (Paraffin) to suggest an etiologic role of METTL23 in NTG with tissue-specific pathology.

Tue Nov 01 00:00:00 UTC 2022

METTL23 mutation alters histone H3R17 methylation in normal-tension glaucoma.

The Journal of clinical investigation

Pan Y,Suga A,Kimura I,Kimura C,Minegishi Y,Nakayama M,Yoshitake K,Iejima D,Minematsu N,Yamamoto M,Mabuchi F,Takamoto M,Shiga Y,Araie M,Kashiwagi K,Aihara M,Nakazawa T,Iwata T

PA5-71814 was used in Western Blot, Immunohistochemistry (Paraffin) to suggest an etiologic role of METTL23 in NTG with tissue-specific pathology.

Tue Nov 01 00:00:00 UTC 2022

METTL23 mutation alters histone H3R17 methylation in normal-tension glaucoma.

The Journal of clinical investigation

Pan Y,Suga A,Kimura I,Kimura C,Minegishi Y,Nakayama M,Yoshitake K,Iejima D,Minematsu N,Yamamoto M,Mabuchi F,Takamoto M,Shiga Y,Araie M,Kashiwagi K,Aihara M,Nakazawa T,Iwata T

PA5-71814 was used in Western Blot, Immunohistochemistry (Paraffin) to suggest an etiologic role of METTL23 in NTG with tissue-specific pathology.

Tue Nov 01 00:00:00 UTC 2022

METTL23 mutation alters histone H3R17 methylation in normal-tension glaucoma.

The Journal of clinical investigation

Pan Y,Suga A,Kimura I,Kimura C,Minegishi Y,Nakayama M,Yoshitake K,Iejima D,Minematsu N,Yamamoto M,Mabuchi F,Takamoto M,Shiga Y,Araie M,Kashiwagi K,Aihara M,Nakazawa T,Iwata T

PA5-71814 was used in Western Blot, Immunohistochemistry (Paraffin) to suggest an etiologic role of METTL23 in NTG with tissue-specific pathology.

Tue Nov 01 00:00:00 UTC 2022

METTL23 mutation alters histone H3R17 methylation in normal-tension glaucoma.

The Journal of clinical investigation

Pan Y,Suga A,Kimura I,Kimura C,Minegishi Y,Nakayama M,Yoshitake K,Iejima D,Minematsu N,Yamamoto M,Mabuchi F,Takamoto M,Shiga Y,Araie M,Kashiwagi K,Aihara M,Nakazawa T,Iwata T

PA5-71814 was used in Western Blot, Immunohistochemistry (Paraffin) to suggest an etiologic role of METTL23 in NTG with tissue-specific pathology.

Tue Nov 01 00:00:00 UTC 2022

生物信息学

蛋白别名: Histone-arginine methyltransferase METTL23; Methyltransferase-like protein 23

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基因别名: C17orf95; METTL23; MRT44

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UniProt ID: (Human) Q86XA0

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Entrez Gene ID: (Human) 124512

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methyltransferase activity

参与通路

methylation