Invitrogen

METTL3 Polyclonal Antibody

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METTL3 Antibody in Western Blot (WB) — Western blot analysis of METTL3 in Lane 1: human placenta tissue lysates, Lane 2: human HepG2 whole cell lysate, Lane 3: human K562 whole cell lysate, Lane 4: human HeLa whole cell lysate, Lane 5: human Caco-2 whole cell lysate. Electrophoresis was performed with 5-20% SDS-PAGE gel (70V, Stacking gel; 90V Resolving gel, Time: 2-3 hours), transferred to a nitrocellulose membrane and blocked using 5% Non-fat Milk/TBS (1.5 hrs at room temperature). Samples were incubated with METTL3 polyclonal antibody (Product # PA5-95569) using a 0.5 µg/mL dilution, followed by a goat anti-rabbit IgG-HRP at a dilution of 1:10,000, and developed with enhanced chemiluminescence (ECL).

METTL3 Antibody in Flow Cytometry (Flow) — Flow Cytometry of METTL3 in HEPA1-6 cells (blue line), isotype control rabbit IgG (green line) and unlabeled (red line). Samples were blocked with 10% goat serum, incubated with METTL3 Polyclonal Antibody (Product # PA5-95569) at a dilution of 1 μg (per 1x10^6 cells), followed by DyLight®488 conjugated goat anti-rabbit IgG (for 30 minutes at 20°C) using 5-10 μg (per 1x10^6 cells) dilution.

产品信息

PA5-95569

应用

建议稀释比

已发表文章

免疫印迹 (WB)

0.1-0.5 µg/mL

流式细胞分析 (Flow)

1-3 µg/1x10^6 cells

酶联免疫吸附实验 (ELISA)

0.1-0.5 µg/mL

产品规格

种属反应

Human, Mouse, Rat

宿主/亚型

Rabbit / IgG

分类

Polyclonal

类型

Antibody

抗原

E.coli-derived human METTL3 recombinant protein (Position: Q423-D571).

查看免疫原

偶联物

Unconjugated

形式

Lyophilized

浓度

500 µg/mL

纯化类型

Affinity chromatography

保存液

PBS with 4mg trehalose

内含物

no preservative

保存条件

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

RRID

AB_2807371

产品详细信息

Reconstitute with 0.2 mL of distilled water to yield a concentration of 500 µg/mL.

靶标信息

METTL3 is the 70 kDa subunit of MT-A which is part of N6-adenosine-methyltransferase. This enzyme is involved in the posttranscriptional methylation of internal adenosine residues in eukaryotic mRNAs, forming N6-methyladenosine.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

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生物信息学

蛋白别名: adoMet-binding subunit of the human mRNA (N6-adenosine)-methyltransferase; hMETTL3; m6a methyltransferase; methyltransferase-like 3; Methyltransferase-like protein 3; mRNA (2'-O-methyladenosine-N(6)-)-methyltransferase; mRNA m(6)A methyltransferase; MT-A70; N6-adenosine-methyltransferase 70 kDa subunit; N6-adenosine-methyltransferase catalytic subunit; N6-adenosine-methyltransferase subunit METTL3

基因别名: 2310024F18Rik; IME4; M6A; METTL3; MT-A70; MTA70; Spo8

UniProt ID: (Human) Q86U44, (Mouse) Q8C3P7

Entrez Gene ID: (Human) 56339, (Mouse) 56335, (Rat) 361035

分子生物学功能: RNA metabolism protein RNA methyltransferase nucleic acid metabolism protein