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Invitrogen
MHC Class I (H-2Kb) Monoclonal Antibody (AF6-88.5.5.3), Biotin, eBioscience™
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图: 1 / 12
MHC Class I (H-2Kb) Antibody (13-5958-80) in Flow
Staining of C57Bl/6 splenocytes with 0.06 µg of Mouse IgG2a K Isotype Control Biotin (Product # 13-4724-85) (left) or 0.06 µg of Anti-Mouse MHC Class I (H-2Kb) Biotin (right) followed by Streptavidin PE (Product # 12-4317-87). Total viable cells were used for analysis.
产品信息
13-5958-80
应用
建议稀释比
已发表文章
产品规格
已发表种属
C. elegans,
Human,
Mouse
宿主/亚型
Mouse
/ IgG2a, kappa
分类
Monoclonal
类型
Antibody
克隆号
AF6-88.5.5.3
偶联物
形式
Liquid
浓度
0.5 mg/mL
保存条件
4°C, store in dark, DO NOT FREEZE!
运输条件
Ambient (domestic); Wet ice (international)
RRID
产品详细信息
Description: This AF6-88.5.5.3 monoclonal antibody reacts with the H-2Kb MHC class I alloantigen. H-2Kb is involved in antigen presentation to T cells expressing CD3/TCR and CD8. Reactivity to other haplotypes (e.g. d, f, j, k, p, q, r, s, u, and v) has not been observed.
Applications Reported: This AF6-88.5.5.3 antibody has been reported for use in flow cytometric analysis.
Applications Tested: This AF6-88.5.5.3 antibody has been tested by flow cytometric analysis on C57Bl/6 splenocytes. This can be used at less than or equal to 0.06 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.
Filtration: 0.2 µm post-manufacturing filtered.
靶标信息
This protein is an MHC class I alloantigen, which is involved in antigen presentation to T cells expressing CD3/TCR and CD8.
HLA and MHC antibodies play a significant role in Immunopeptidomics, facilitating the identification and characterization of neoantigens through high-performance liquid chromatography coupled to tandem Mass Spectrometry.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
