Invitrogen

MHC Class II (H2-Aa) Polyclonal Antibody

Name: MHC Class II (H2-Aa) Polyclonal Antibody
Brand: Invitrogen
SKU: PA5-116820

说明书

实验方案

问题与答案

技术支持

说明书

实验方案

问题与答案

技术支持

说明书

实验方案

问题与答案

技术支持

抗体检测数据 (1)

MHC Class II (H2-Aa) Antibody in Western Blot (WB)

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MHC Class II (H2-Aa) Antibody (PA5-116820) in WB

Western blot analysis of MHC Class II (H2-Aa) in extracts of various cell lines. Samples were incubated with MHC Class II (H2-Aa) Polyclonal antibody (Product # PA5-116820) using a dilution of 1:1,000, followed by HRP Goat Anti-Rabbit IgG (H+L) at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 60s.

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Immunogen image

Immunogen Image MHC Class II (H2-Aa) Polyclonal Antibody

产品信息

PA5-116820

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1:500-1:1,000

酶联免疫吸附实验 (ELISA)

1 µg/mL

产品规格

种属反应

Human, Mouse

宿主/亚型

Rabbit / IgG

分类

Polyclonal

类型

Antibody

抗原

Recombinant protein (or fragment)

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偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

1.99 mg/mL

纯化类型

Affinity chromatography

保存液

PBS, pH 7.3, with 50% glycerol

内含物

0.09% sodium azide

保存条件

-20°C, Avoid Freeze/Thaw Cycles

运输条件

Ambient (domestic); Wet ice (international)

AB_2901451

产品详细信息

Positive Samples: Mouse lung, Mouse kidney

Immunogen sequence: IEADHVGTYG ISVYQSPGDI GQYTFEFDGD ELFYVDLDKK ETVWMLPEFG QLASFDPQGG LQNIAVVKHN LGVLTKRSNS TPATNEAPQA TVFPKSPVLL GQPNTLICFV DNIFPPVINI TWLRNSKSVA DGVYETSFFV NRDYSFHKLS YLTFIPSDDD IYDCKVEHWG LEEPVLKHWE PE

靶标信息

MHC class II molecules are heterodimers of non-covalently associated alpha (31-34 kDa) and beta (26-29 kDa) chains. Major histocompatibility complex class II antigen presentation requires the participation of lysosomal proteases in two convergent processes. First, the antigens endocytosed by the antigen presenting cells must be broken down into antigenic peptides. Second, class II molecules are synthesized with their peptide-binding site blocked by invariant chain (Ii), and they aquire the capacity to bind antigens only after Ii has been degraded in the compartments where peptides reside. MHC class II molecules present exogenously derived antigen to CD4+ T lymphocytes, which are usually T helper cells. CD4 interacts with non-polymorphic residues of MHC class II.

HLA and MHC antibodies play a significant role in Immunopeptidomics, facilitating the identification and characterization of neoantigens through high-performance liquid chromatography coupled to tandem Mass Spectrometry.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

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引用该产品

生物信息学

蛋白别名: A alpha; H-2 class II histocompatibility antigen, A-B alpha chain; H-2 class II histocompatibility antigen, A-F alpha chain; H-2 class II histocompatibility antigen, A-K alpha chain; H-2 class II histocompatibility antigen, A-Q alpha chain; H-2 class II histocompatibility antigen, A-R alpha chain; H-2 class II histocompatibility antigen, A-S alpha chain; H-2 class II histocompatibility antigen, A-U alpha chain; histocompatibility 2, class II antigen A, alpha; IAalpha; major histocompatibility protein class II alpha chain; MHC class II antigen A alpha isoform-004; MHC-II I-A(d) alpha

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基因别名: Aalpha; H-2Aa; H2-Aa; H2Aa; I-Aalpha; Ia-1; Ia1; IAalpha

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UniProt ID: (Mouse) P14434

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Entrez Gene ID: (Mouse) 14960

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功能

protein binding peptide antigen binding protein heterodimerization activity major histocompatibility complex protein

参与通路

immune system process antigen processing and presentation of peptide or polysaccharide antigen via MHC class II immune response antigen processing and presentation antigen processing and presentation of exogenous peptide antigen via MHC class II response to interferon-gamma negative regulation of T cell proliferation positive regulation of T cell differentiation antigen processing and presentation of peptide antigen