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AA Sequence of recombinant protein: QITHATETKE VQSSLKAQQG LEIEMFHMGF QDSSDCCLSY NSRIQCSRFI GYFPTSGGCT RPGIIFISKR GFQVCANPSD RRVQRCIERL EQNSQPRTYK Q.
Preparation: Produced from sera of rabbits immunized with highly pure Recombinant Murine MIP-1gamma (CCL9/10). Anti-Murine MIP-1gamma (CCL9/10)-specific antibody was purified by affinity chromatography and then biotinylated.
Sandwich ELISA: To detect Murine MIP-1gamma (CCL9/10) by sandwich ELISA (using 100 µL/well antibody solution) a concentration of 0.25-1.0 µg/mL of this antibody is required. This biotinylated polyclonal antibody, in conjunction with PeproTech Polyclonal Anti-Murine MIP-1gamma (CCL9/10) (500-P117) as a capture antibody, allows the detection of at least 0.2-0.4 ng/well of Recombinant Murine MIP-1gamma (CCL9/10).
Western Blot: To detect Murine MIP-1gamma by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 µg/mL. When used in conjunction with compatible secondary reagents, the detection limit for Recombinant Murine MIP-1gamma is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.
500-P117BT-1MG will be provided as 2 x 500 µg
MIP-1gamma is a CC chemokine found in murine blood and a wide variety of murine tissues, with no known human homolog. MIP-1gamma signals through the CCR1 receptor. MIP-1gamma chemoattracts neutrophils, and also inhibits colony formation of bone marrow myeloid immature progenitors. MIP-1gamma contains six cysteines, including the four highly conserved cysteine residues present in CC chemokines.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
蛋白别名: C-C motif chemokine 9; CCF18; Macrophage inflammatory protein 1-gamma; Macrophage inflammatory protein-related protein 2; MIP-1 gamma; MIP-1-gamma; MRP-2; RP23-320E6.3; small inducible cytokine A10; small inducible cytokine A9; Small-inducible cytokine A9
基因别名: CCF18; Ccl9; MRP-2; Mrp2; Scya10; Scya9
UniProt ID: (Mouse) P51670
Entrez Gene ID: (Mouse) 20308