Invitrogen

MLH1 Recombinant Rabbit Monoclonal Antibody (PD01-61)

Name: MLH1 Recombinant Rabbit Monoclonal Antibody (PD01-61)
Brand: Invitrogen
SKU: MA5-50815
Availability: InStock

查看其他44个MLH1抗体

说明书

Certificates

实验方案

问题与答案

技术支持

说明书

Certificates

实验方案

问题与答案

技术支持

说明书

Certificates

实验方案

问题与答案

技术支持

Additional Information:

抗体检测数据 (5)

MLH1 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

图: 1 / 5

MLH1 Antibody (MA5-50815) in IHC (P)

Immunohistochemistry of MLH1 in paraffin-embedded human colon carcinoma tissue. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with MLH1 recombinant monoclonal antibody (Product # MA5-50815) with a dilution of 1:1,000 for 1 ho... View More

Please note: We are reviewing Western blot images included in the antibody testing data in our catalog, including those provided by third parties. Unless expressly labeled or annotated as “raw-unedited”, Western blot images included in the antibody testing data in our catalog may have been edited, optimized or otherwise adjusted for presentation.

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Immunogen image

Immunogen Image MLH1 Recombinant Rabbit Monoclonal Antibody (PD01-61)

产品信息

MA5-50815

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1:1,000

免疫组化（石蜡） (IHC (P))

1:200-1:1,000

产品规格

种属反应

Human, Rat

宿主/亚型

Rabbit / IgG

Expression System

HEK293 cells

分类

Recombinant Monoclonal

类型

Antibody

克隆号

PD01-61

抗原

Synthetic peptide corresponding to Human MLH1 aa 700-756.

3D表位/免疫原

偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

1 mg/mL

100 µg

纯化类型

Protein A

保存液

PBS, pH 7.4, with 0.1% BSA, 40% glycerol

内含物

0.05% sodium azide

保存条件

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

运输条件

Ambient (domestic); Wet ice (international)

AB_3093794

产品详细信息

Positive control: K562 cell lysates, human colon tissue, rat small intestine tissue, human colon carcinoma tissue, human tonsil tissue.

Predicted band size: 85 kDa

Subcellular Location: Nucleus.

靶标信息

MLH1 is a DNA mismatch repair protein. The repair of mismatch DNA is essential to maintaining the integrity of genetic information over time. An alteration of microsatellite repeats is the result of slippage owing to strand misalignment during DNA replication and is referred to as microsatellite instability (MSI). These defects in DNA repair pathways have been related to human carcinogenesis. The importance of mismatch repair genes became apparent with the identification of the genetic basis for hereditary nonpolyposis colon cancer (HNPC). MSHS2 is involved in the initial cognition of mismatch nucleotides during the replication mismatch repair process. It is thought that after MSH2 binds to a mismatched DNA duplex it is joined by a heterodimer of MLH1 and PMSH, which together help facilitate the later steps in mismatch repair.

仅用于科研。不用于诊断过程。未经明确授权不得转售。