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MMP13 Antibody (PA5-95401) in WB
Western blot analysis of MMP13 in Lane 1: human PC-3 whole cell lysates, Lane 2: human U20S whole cell lysates, Lane 3: human A549 whole cell lysates, Lane 4: human HEK293 whole cell lysates. Electrophoresis was performed with 5-20% SDS-PAGE gel (70V, Stacking gel; 90V Resolving gel, Time: 2-3 hours), transferred to a nitrocellulose membrane and blocked using 5% Non-fat Milk/TBS (1.5 hrs at room temperature). Samples were incubated with MMP13 polyclonal antibody (Product # PA5-95401) using a 0.5 µg/mL dilution, followed by a goat anti-rabb... View More
Product Details
PA5-95401
产品规格
Species Reactivity
Human,
Non-human primate
Host/Isotype
Rabbit
/ IgG
分类
Polyclonal
类型
Antibody
Immunogen
A synthetic peptide corresponding to a sequence at the N-terminus of human MMP13 (109-154aa RTLKWSKMNLTYRIVNYTPDMTHSEVEKAFKKAFKVWSDVTPLNFT).
Conjugate
Unconjugated
形式
Lyophilized
浓度
500 µg/mL
纯化类型
Affinity chromatography
保存液
PBS with 4mg trehalose
内含物
0.05mg sodium azide
保存条件
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
RRID
AB_2807204
Product Specific Information
Reconstitute with 0.2 mL of distilled water to yield a concentration of 500 µg/mL.
Target Information
MMP13 (collagense 3) plays a role in the degradation of extracellular matrix proteins including fibrillar collagen, fibronectin, TNC, and ACAN. MMP13 cleaves tripal helical collagens, including type I, II, and III collagen - but has the highest activity with soluble type II collagen. It can degrade collagen type IV, XIV, and X. MMP13 plays a role in wound healing, tissue remodeling, cartilage degradation, bone development, bone mineralization, and ossification. It is required for normal embryonic bone development and ossificaiton. Mutations affecting the gene can lead to spondyloepimetaphyseal dysplasia Missouri type, metaphyseal anadysplasia 1, and metaphyseal dysplasia Sphar type.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
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Bioinformatics
Protein Aliases: Collagenase; Collagenase 3; Collagenase3; matrix metallo protease; matrix metalloproteinase 13 (collagenase 3); Matrix metalloproteinase-13; MMP; MMP-13; MMPs; Procollagenase 3
Gene Aliases: CLG3; MANDP1; MMP-13; MMP13
UniProt ID: (Human) P45452
Entrez Gene ID: (Human) 4322
Molecular Function:
metalloprotease
luteolysis
osteoblast differentiation
response to hypoxia
endochondral ossification
growth plate cartilage development
proteolysis
heart development
parturition
response to mechanical stimulus
extracellular matrix disassembly
bone mineralization
collagen catabolic process
embryonic hindlimb morphogenesis
response to drug
peptide catabolic process
response to estrogen
cellular protein metabolic process
estrous cycle
bone morphogenesis
cellular response to fluid shear stress
positive regulation of pancreatic trypsinogen secretion
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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