Invitrogen

MMP13 Recombinant Rabbit Monoclonal Antibody (3H13L17)

MMP13 Antibody in Immunocytochemistry (ICC/IF)

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MMP13 Antibody (701287) in ICC/IF

Immunofluorescent analysis of MMP-13 in HeLa cells using a MMP-13 recombinant rabbit monoclonal antibody (Product # 701287) followed by detection using an Alexa Fluor 488-conjugated goat anti-rabbit secondary antibody (green) (Image A). Nuclei were stained using DAPI (Image B) and actin stained with Alexa Fluor 594 phalloidin (red) (image C). Image D is a composite image showing nuclear localization of MMP-13.

MMP13 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

MMP13 Antibody in Western Blot, Immunocytochemistry (WB, ICC/IF)

MMP13 Antibody in Immunohistochemistry (IHC)

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产品信息

701287

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1-3 µg/mL

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免疫组化 (IHC)

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免疫组化（石蜡） (IHC (P))

1:20-1:200

免疫细胞化学 (ICC/IF)

1 µg/mL

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产品规格

种属反应

Human

已发表种属

Human, Mouse

宿主/亚型

Rabbit / IgG

Expression System

Expi293

分类

Recombinant Monoclonal

类型

Antibody

克隆号

3H13L17

抗原

Recombinant protein corresponding to amino acids 221-471 of human MMP-13

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偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

0.5 mg/mL

纯化类型

Protein A

保存液

PBS

内含物

0.09% sodium azide

保存条件

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

运输条件

Wet ice

RRID

AB_2532459

产品详细信息

This antibody is predicted to react with mouse, rat, non-human primate and rabbit based on sequence homology.

Intact IgG appears on a non-reducing gel as ~150 kDa band and upon reduction generating a ~25 kDa light chain band and a ~50 kDa heavy chain.

Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems. The expression systems are developed by cloning in the specific antibody DNA sequences from immunoreactive rabbits. Then, individual clones are screened to select the best candidates for production. The advantages of using recombinant rabbit monoclonal antibodies include: better specificity and sensitivity, lot-to-lot consistency, animal origin-free formulations, and broader immunoreactivity to diverse targets due to larger rabbit immune repertoire.

靶标信息

MMP13 (collagense 3) plays a role in the degradation of extracellular matrix proteins including fibrillar collagen, fibronectin, TNC, and ACAN. MMP13 cleaves tripal helical collagens, including type I, II, and III collagen - but has the highest activity with soluble type II collagen. It can degrade collagen type IV, XIV, and X. MMP13 plays a role in wound healing, tissue remodeling, cartilage degradation, bone development, bone mineralization, and ossification. It is required for normal embryonic bone development and ossificaiton. Mutations affecting the gene can lead to spondyloepimetaphyseal dysplasia Missouri type, metaphyseal anadysplasia 1, and metaphyseal dysplasia Sphar type.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

Product References

Glucosidase inhibitor, Nimbidiol ameliorates renal fibrosis and dysfunction in type-1 diabetes.

Scientific reports

Juin SK,Pushpakumar S,Tyagi SC,Sen U

701287 was used in Western Blot to suggest that Nimbidiol attenuates inflammation and ECM accumulation and thereby, protects kidney from fibrosis and dysfunction possibly by inhibiting TGF-β/Smad and MAPK signaling pathways in type-1 diabetes.

Thu Dec 15 00:00:00 EST 2022

Xanthohumol Attenuated Inflammation and ECM Degradation by Mediating HO-1/C/EBPβ Pathway in Osteoarthritis Chondrocytes.

Frontiers in pharmacology

Zhang M,Zhang R,Zheng T,Chen Z,Ji G,Peng F,Wang W

Published figure using MMP13 recombinant monoclonal antibody (Product # 701287) in Immunocytochemistry

Sun May 23 00:00:00 EDT 2021

Xanthohumol Attenuated Inflammation and ECM Degradation by Mediating HO-1/C/EBPβ Pathway in Osteoarthritis Chondrocytes.

Frontiers in pharmacology

Zhang M,Zhang R,Zheng T,Chen Z,Ji G,Peng F,Wang W

Published figure using MMP13 recombinant monoclonal antibody (Product # 701287) in Immunocytochemistry

Sun May 23 00:00:00 EDT 2021

Xanthohumol Attenuated Inflammation and ECM Degradation by Mediating HO-1/C/EBPβ Pathway in Osteoarthritis Chondrocytes.

Frontiers in pharmacology

Zhang M,Zhang R,Zheng T,Chen Z,Ji G,Peng F,Wang W

Published figure using MMP13 recombinant monoclonal antibody (Product # 701287) in Immunocytochemistry

Sun May 23 00:00:00 EDT 2021

Investigation of the role of matrix metalloproteinases in the genetic etiology of Alzheimer's disease.

Neurobiology of aging

Hoogmartens J,Hens E,Engelborghs S,De Deyn PP,van der Zee J,Van Broeckhoven C,Cacace R

701287 was used in Immunocytochemistry, Immunohistochemistry (Paraffin), Western Blot to hypothesize a gain-of-function disease mechanism for the missense mutations found in patients.

Sun Aug 01 00:00:00 EDT 2021

GYY4137 Regulates Extracellular Matrix Turnover in the Diabetic Kidney by Modulating Retinoid X Receptor Signaling.

Biomolecules

Juin SK,Pushpakumar S,Sen U

701287 was used in Western Blot to suggest that RXR signaling plays a significant role in ECM turnover, and GYY4137 modulates PPAR/RAR-mediated RXR signaling to ameliorate PAI-1-dependent adverse ECM turnover in DN.

Thu Oct 07 00:00:00 EDT 2021

Distinct biological events generated by ECM proteolysis by two homologous collagenases.

Proceedings of the National Academy of Sciences of the United States of America

Solomonov I,Zehorai E,Talmi-Frank D,Wolf SG,Shainskaya A,Zhuravlev A,Kartvelishvily E,Visse R,Levin Y,Kampf N,Jaitin DA,David E,Amit I,Nagase H,Sagi I

Published figure using MMP13 monoclonal antibody (Product # 701287) in Western Blot

Tue Sep 27 00:00:00 EDT 2016

生物信息学

蛋白别名: Collagenase; Collagenase 3; Collagenase3; matrix metallo protease; matrix metalloproteinase 13 (collagenase 3); Matrix metalloproteinase-13; MMP; MMP-13; MMPs; Procollagenase 3

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基因别名: CLG3; MANDP1; MMP-13; MMP13

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UniProt ID: (Human) P45452

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Entrez Gene ID: (Human) 4322

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功能

fibronectin binding metalloendopeptidase activity serine-type endopeptidase activity calcium ion binding collagen binding zinc ion binding calcium-dependent protein binding low-density lipoprotein particle receptor binding metalloprotease

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