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          Disclaimer

          Clicking the images or links will redirect you to a website hosted by BenchSci that provides third-party scientific content. Neither the content nor the BenchSci technology and processes for selection have been evaluated by us; we are providing them as-is and without warranty of any kind, including for use or application of the Thermo Fisher Scientific products presented.

          • Primary Antibodies ›
          • MMP13 Antibodies

          Invitrogen

          MMP13 Monoclonal Antibody (VIIIA2)

          12个已发表图片
          52 篇参考文献
          查看其他20个MMP13抗体

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          说明书
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          技术支持
          说明书
          实验方案
          问题与答案
          技术支持

          Cite MMP13 Monoclonal Antibody (VIIIA2)

          • 抗体检测数据 (2)
          • 已发表图片 (12)
          MMP13 Antibody in Western Blot (WB)
          Group 53 Created with Sketch.
          MMP13 Antibody in Western Blot (WB)
          Group 53 Created with Sketch.

          图: 1 / 14

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          MMP13 Antibody (MA5-14238) in WB

          Western blot was performed using Anti-MMP13 Monoclonal Antibody (VIIIA2) (Product # MA5-14238) and a 50 kDa band corresponding to the activated form of MMP-13 was observed in all the tested cell lines. Whole cell lysates (30 µg lysate) of HeLa (Lane 1), COLO 205 (Lane 2), MDA-MB-231 (Lane 3), Jurkat (Lane 4), PC-3 (Lane 5), U-2 OS (Lane 6) and Hep G2 (Lane 7) were electrophoresed using NuPAGE® 10 % Bis-Tris gel (Product # NP0302BOX). Resolved proteins were then transferred onto a nitrocellulose membrane (Product # IB23001) by iBlot® 2 Dry ... View More {{ $ctrl.currentElement.advancedVerification.fullName }} 验证信息 View more
          已发表图片由___提供 benchsci-logo
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          查看产品

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          MMP13 Antibody in Western Blot (WB)
          MMP13 Antibody in Western Blot (WB)
          MMP13 Antibody in Immunohistochemistry (IHC)
          MMP13 Antibody in Immunocytochemistry (ICC/IF)
          MMP13 Antibody in Immunocytochemistry (ICC/IF)
          MMP13 Antibody in Immunohistochemistry (Paraffin) (IHC (P))
          MMP13 Antibody in Immunohistochemistry (IHC)
          MMP13 Antibody in Immunohistochemistry (IHC)
          MMP13 Antibody in Immunohistochemistry (IHC)
          MMP13 Antibody in Immunohistochemistry (IHC)
          MMP13 Antibody in Immunohistochemistry (IHC)
          MMP13 Antibody in Western Blot (WB)
          MMP13 Antibody in Western Blot (WB)
          MMP13 Antibody in Western Blot (WB)

          产品信息

          MA5-14238

          应用
          建议稀释比
          已发表文章

          免疫印迹 (WB)

          2-4 µg/mL
          查看6篇已发表文章 6篇已发表文章

          免疫组化 (IHC)

          -
          查看39篇已发表文章 39篇已发表文章

          免疫组化(石蜡) (IHC (P))

          -
          查看4篇已发表文章 4篇已发表文章

          免疫细胞化学 (ICC/IF)

          -
          查看3篇已发表文章 3篇已发表文章
          产品规格

          种属反应

          Human, Mouse

          已发表种属

          Human, Mouse, Rabbit, Rat

          宿主/亚型

          Mouse / IgG1, kappa

          分类

          Monoclonal

          类型

          Antibody

          克隆号

          VIIIA2

          抗原

          Human recombinant collagenase-3 protein

          偶联物

          Unconjugated Unconjugated Unconjugated

          形式

          Liquid

          浓度

          0.2 mg/mL

          纯化类型

          Protein G

          保存液

          PBS, pH 7.4, with 0.2% BSA

          内含物

          0.09% sodium azide

          保存条件

          4°C

          运输条件

          Ambient (domestic); Wet ice (international)

          RRID

          AB_10981616

          产品详细信息

          MA5-14238 targets MMP-13 (Collagenase-3) in IHC (P) and IP applications and shows reactivity with Human, mouse, and Rabbit samples.

          The MA5-14238 immunogen is human recombinant collagenase-3 protein.

          靶标信息

          MMP13 (collagense 3) plays a role in the degradation of extracellular matrix proteins including fibrillar collagen, fibronectin, TNC, and ACAN. MMP13 cleaves tripal helical collagens, including type I, II, and III collagen - but has the highest activity with soluble type II collagen. It can degrade collagen type IV, XIV, and X. MMP13 plays a role in wound healing, tissue remodeling, cartilage degradation, bone development, bone mineralization, and ossification. It is required for normal embryonic bone development and ossificaiton. Mutations affecting the gene can lead to spondyloepimetaphyseal dysplasia Missouri type, metaphyseal anadysplasia 1, and metaphyseal dysplasia Sphar type.

          仅用于科研。不用于诊断过程。未经明确授权不得转售。

          生物信息学

          蛋白别名: Collagenase; Collagenase 3; collagenase-1; Collagenase-3; Collagenase3; interstitial collagenase; matrix metallo protease; matrix metalloproteinase 13; matrix metalloproteinase 13 (collagenase 3); Matrix metalloproteinase-13; MMP; MMP-13; MMPs; Procollagenase 3

          查看更多 收起

          基因别名: Clg; CLG3; MANDP1; MMP-13; Mmp1; MMP13

          查看更多 收起

          UniProt ID: (Human) P45452, (Mouse) P33435

          查看更多 收起

          Entrez Gene ID: (Human) 4322, (Mouse) 17386

          查看更多 收起

          功能
          fibronectin binding metalloendopeptidase activity serine-type endopeptidase activity calcium ion binding collagen binding zinc ion binding calcium-dependent protein binding low-density lipoprotein particle receptor binding peptidase activity metallopeptidase activity hydrolase activity metal ion binding metalloprotease
          参与通路
          luteolysis osteoblast differentiation response to hypoxia endochondral ossification growth plate cartilage development proteolysis heart development parturition response to mechanical stimulus extracellular matrix disassembly bone mineralization collagen catabolic process embryonic hindlimb morphogenesis response to drug peptide catabolic process response to estrogen cellular protein metabolic process estrous cycle bone morphogenesis cellular response to fluid shear stress positive regulation of pancreatic trypsinogen secretion receptor internalization cartilage development
          It has to be done as per old AB suggested Products section.
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          性能保障

          只要您发现Invitrogen抗体在实验中的表现与网站或说明书的所述内容不符,您即可享受免费退换货服务。*

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