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Invitrogen
MRPL42 Polyclonal Antibody
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MRPL42 Antibody (PA5-120186) in WB
Western blot analysis of MRPL42 in extracts of various cell lines. Samples were incubated with MRPL42 Polyclonal antibody (Product # PA5-120186) using a dilution of 1:3,000, followed by HRP Goat Anti-Rabbit IgG (H+L) at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.
产品信息
PA5-120186
产品规格
种属反应
Human,
Mouse
宿主/亚型
Rabbit
/ IgG
分类
Polyclonal
类型
Antibody
抗原
Recombinant fusion protein containing a sequence corresponding to amino acids 1-142 of human MRPL42 (NP_054769.1).
偶联物
形式
Liquid
浓度
0.84 mg/mL
纯化类型
Affinity Chromatography
保存液
PBS, pH 7.3, with 50% glycerol
内含物
0.01% thimerosal
保存条件
-20°C, Avoid Freeze/Thaw Cycles
运输条件
Ambient (domestic); Wet ice (international)
RRID
产品详细信息
Positive test controls include: U-87MG, HeLa, Raji, HepG2, U-251MG, Mouse kidney. The target is usually found in the following locations: Mitochondrion.
Immunogen sequence: MAVAAVKWVM SKRTILKHLF PVQNGALYCV CHKSTYSPLP DDYNCNVELA LTSDGRTIVC YHPSVDIPYE HTKPIPRPDP VHNNEETHDQ VLKTRLEEKV EHLEEGPMIE QLSKMFFTTK HRWYPHGRYH RCRKNLNPPK DR
靶标信息
MRPL42 are mammalian mitochondrial ribosomal proteins are encoded by nuclear genes and help in protein synthesis within the mitochondrion. Mitochondrial ribosomes (mitoribosomes) consist of a small 28S subunit and a large 39S subunit. They have an estimated 75% protein to rRNA composition compared to prokaryotic ribosomes, where this ratio is reversed. Another difference between mammalian mitoribosomes and prokaryotic ribosomes is that the latter contain a 5S rRNA. Among different species, the proteins comprising the mitoribosome differ greatly in sequence, and sometimes in biochemical properties, which prevents easy recognition by sequence homology. This gene encodes a protein identified as belonging to both the 28S and the 39S subunits. Alternative splicing results in multiple transcript variants. Pseudogenes corresponding to this gene are found on chromosomes 4q, 6p, 6q, 7p, and 15q.
⚠WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause birth defects or other reproductive harm. For more information go to www.P65Warnings.ca.gov.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
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生物信息学
蛋白别名: 28S ribosomal protein L42, mitochondrial; 28S ribosomal protein S32, mitochondrial; 39S ribosomal protein L31, mitochondrial; 39S ribosomal protein L42, mitochondrial; L31mt; L42mt; Large ribosomal subunit protein mL42; mitochndrial ribosomal protein L42; MRP-L42; MRP-S32; S32mt
基因别名: 2700009F22Rik; 2900055D03Rik; D10Ertd322e; HSPC204; L31MT; L42MT; MRP-L31; MRP-L42; MRP-S32; MRPL31; MRPL42; MRPS32; PTD007; RPML31; S32MT
UniProt ID: (Human) Q9Y6G3, (Mouse) Q9CPV3
Entrez Gene ID: (Human) 28977, (Mouse) 67270
