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Invitrogen
MTM1 Polyclonal Antibody
Advanced Verification
This Antibody was verified by Knockdown to ensure that the antibody binds to the antigen stated.
FIGURE: 1 / 9
MTM1 Antibody (PA5-17972) in WB
Western blot analysis was performed membrane enriched extracts (30 µg lysate) of Hep G2 (Lane 1), HEK-293 (Lane 2), A-431 (Lane 3), A549 (Lane 4), HeLa (Lane 5) and tissue extract of Mouse Skeletal Muscle (Lane 6). The blot was probed with Anti-MTM1 antibody (Product # PA5-17972, 1µg/mL) and detected by chemiluminescence using Rabbit anti-Goat IgG Heavy Chain Superclonal™ Secondary Antibody, HRP conjugate (Product # A27014, 0.25 µg/mL, 1:4000 dilution). A 62 kDa band corresponding to MTM1 was observed across the cell lines and tissue teste... View More
Product Details
PA5-17972
Applications
Tested Dilution
Publications
产品规格
Species Reactivity
Human,
Mouse
Published species
Human
Host/Isotype
Goat
/ IgG
分类
Polyclonal
类型
Antibody
Immunogen
Synthetic peptide sequence (SSPSQMMPHVQTHF) corresponding to the C-terminus amino acids of MTM1
Conjugate
Unconjugated
形式
Liquid
浓度
0.5 mg/mL
纯化类型
Antigen affinity chromatography
保存液
TBS, pH 7.3, with 0.5% BSA
内含物
0.02% sodium azide
保存条件
-20° C, Avoid Freeze/Thaw Cycles
RRID
AB_11002306
Product Specific Information
This antibody is predicted to react with bovine, canine and mouse based on sequence homology.
Target Information
Human MTM1, a 603 amino-acid protein, is mutated in myotubular myopathy. The largely related protein hMTMR2 is found mutated in a recessive form of Charcot-Marie-tooth neuropathy. Myotubularin is primarily a lipid phosphatase that acts on hosphatidylinositol 3-monophosphate and is involved in the regulation of the phosphatidylinositol 3-kinase (PI 3-kinase) pathway and membrane trafficking. Wildtype myotubularin can directly dephosphorylate PI 3-P and PI 4-P in vitro. Thus, it decreases PI 3-P levels by down-regulating PI 3-K activity and by facilitating the degradation of PI 3-P.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
Bioinformatics
Protein Aliases: Myotubularin; Phosphatidylinositol-3,5-bisphosphate 3-phosphatase; Phosphatidylinositol-3-phosphate phosphatase
Gene Aliases: AF073996; CG2; CNM; mKIAA4176; Mtm; MTM1; MTMX; XLMTM
UniProt ID: (Human) A6NDB1, (Mouse) Q9Z2C5
Entrez Gene ID: (Human) 4534, (Mouse) 17772
Molecular Function:
phosphatase
protein dephosphorylation
phosphatidylinositol biosynthetic process
endosome to lysosome transport
protein transport
negative regulation of TOR signaling
negative regulation of proteasomal ubiquitin-dependent protein catabolic process
peptidyl-tyrosine dephosphorylation
regulation of vacuole organization
intermediate filament organization
muscle cell cellular homeostasis
phosphatidylinositol dephosphorylation
mitochondrion distribution
positive regulation of skeletal muscle tissue growth
negative regulation of protein kinase B signaling
mitochondrion morphogenesis
negative regulation of autophagosome assembly
lipid metabolic process
transport
dephosphorylation
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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