Invitrogen

MTM1 Polyclonal Antibody

高级验证

This Antibody was verified by Knockdown to ensure that the antibody binds to the antigen stated.

查看其他9个MTM1抗体

说明书

实验方案

技术支持

说明书

实验方案

技术支持

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MTM1 Antibody in Western Blot (WB) — Knockdown of MTM 1 was achieved by transfecting Hep G2 cells with MTM 1 specific siRNAs (Silencer® select Product # s9041). Western blot analysis (Fig. a) was performed using whole cell extracts from the MTM 1 knockdown cells (lane 3), non-specific scrambled siRNA transfected cells (lane 2) and untransfected cells (lane 1). The blots were probed with MTM 1 Polyclonal Antibody (Product # PA5-17972, 1:250 dilution) and Goat anti-Rabbit IgG (H+L) Superclonal™ Secondary Antibody, HRP conjugate (Product # A27036, 0.25 µg/mL, 1:4000 dilution). Densitometric analysis of this western blot is shown in histogram (Fig. b). Decrease in signal upon siRNA mediated knock down confirms that antibody is specific to MTM 1.

MTM1 Antibody — Antibody specificity was demonstrated by siRNA mediated knockdown of target protein. Hep G2 cells were transfected with MTM 1 siRNA and loss of signal was observed in Western Blot using Anti-MTM 1 Polyclonal Antibody (Product # PA5-17972). {KD}

产品信息

PA5-17972

应用	建议稀释比	已发表文章
免疫印迹 (WB)	1-3 µg/mL	-
免疫组化（石蜡） (IHC (P))	10 µg/mL	-

产品规格
种属反应	Human, Mouse
宿主/亚型	Goat / IgG
分类	Polyclonal
类型	Antibody
抗原	Synthetic peptide sequence (SSPSQMMPHVQTHF) corresponding to the C-terminus amino acids of MTM1
偶联物	Unconjugated
形式	Liquid
浓度	0.5 mg/mL
纯化类型	Antigen affinity chromatography
保存液	TBS, pH 7.3, with 0.5% BSA
内含物	0.02% sodium azide
保存条件	-20° C, Avoid Freeze/Thaw Cycles
RRID	AB_11002306

产品详细信息

This antibody is predicted to react with bovine, canine and mouse based on sequence homology.

靶标信息

Human MTM1, a 603 amino-acid protein, is mutated in myotubular myopathy. The largely related protein hMTMR2 is found mutated in a recessive form of Charcot-Marie-tooth neuropathy. Myotubularin is primarily a lipid phosphatase that acts on hosphatidylinositol 3-monophosphate and is involved in the regulation of the phosphatidylinositol 3-kinase (PI 3-kinase) pathway and membrane trafficking. Wildtype myotubularin can directly dephosphorylate PI 3-P and PI 4-P in vitro. Thus, it decreases PI 3-P levels by down-regulating PI 3-K activity and by facilitating the degradation of PI 3-P.

仅用于科研。不用于诊断过程。未经明确授权不得转售。