This antibody was purified from whole rabbit serum prepared by repeated immunizations with MYC epitope tag peptide, E-Q-K-L-I-S-E-E-D-L, conjugated to KLH using maleimide. The sequence corresponds to amino acids 410-419 of human c-Myc.

偶联物

DyLight™ 549 DyLight™ 549 DyLight™ 549

激发/发射光谱

556/570 nm 查看光谱

形式

Lyophilized

浓度

1 mg/mL

100 µg

纯化类型

Affinity chromatography

保存液

0.02M potassium phosphate, pH 7.2, with 10mg/mL BSA, 0.15M NaCl

内含物

0.01% sodium azide

保存条件

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

运输条件

Ambient (domestic); Wet ice (international)

产品详细信息

Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

This affinity purified antibody is directed against the MYC epitope tag and is useful in determining its presence in over expressed proteins in various assays. The antibody recognizes the MYC epitope tag -tag (Glu-Gln-Lys-Leu-Ile-Ser-Glu-Glu-Asp-Leu) fused to either the amino- or carboxy- termini of targeted proteins in transfected or transformed cells.

靶标信息

Myc is a proto-oncogene and encodes a nuclear phosphoprotein that plays a role in cell cycle progression, apoptosis and cellular transformation. The encoded protein forms a heterodimer with the related transcription factor MAX. This complex binds to the E box DNA consensus sequence and regulates the transcription of specific target genes. Amplification of this gene is frequently observed in numerous human cancers. Translocations involving this gene are associated with Burkitt lymphoma and multiple myeloma in human patients. There is evidence to show that translation initiates both from an upstream, in-frame non-AUG (CUG) and a downstream AUG start site, resulting in the production of two isoforms with distinct N-termini.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

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引用该产品

生物信息学

蛋白别名: 46-0603; c Myc; c-myc epitope tag; cmyc; Myc protein; Myc Tag; R950-25; R95025

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MYC Epitope Tag Polyclonal Antibody, DyLight™ 549

产品信息

600-442-381

免疫印迹 (WB)

免疫细胞化学 (ICC/IF)

酶联免疫吸附实验 (ELISA)

种属反应

宿主/亚型

分类

类型

抗原

偶联物