Invitrogen

MYH9 Polyclonal Antibody

MYH9 Antibody in Immunocytochemistry (ICC/IF)

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MYH9 Antibody (PA5-17025) in ICC/IF

Immunofluorescent analysis of Myosin IIa in HeLa cells using a Myosin IIa polyclonal antibody (Product # PA5-17025) (green) showing colocalization with actin filaments that have been labeled with a fluorescent red phalloidin. DNA is labeled using a fluorescent blue dye.

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产品信息

PA5-17025

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1:1,000

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免疫细胞化学 (ICC/IF)

1:50

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产品规格

种属反应

Human, Mouse, Rat

已发表种属

Dog, Mouse

宿主/亚型

Rabbit / IgG

分类

Polyclonal

类型

Antibody

抗原

Synthetic peptide corresponding to the carboxy terminus of mouse myosin IIa

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偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

20 µg/mL

纯化类型

Affinity chromatography

保存液

0.01M HEPES, pH 7.5, with 0.15M NaCl, 100µg/mL BSA, 50% glycerol

内含物

no preservative

保存条件

-20°C

运输条件

Ambient (domestic); Wet ice (international)

AB_10984569

产品详细信息

It is not recommended to aliquot this antibody.

This antibody is not cross-reactive with the nonmuscle heavy chains of myosin IIb or IIc.

靶标信息

The MYH9 gene, located on chromosome 22q12.3, encodes the heavy chain of non-muscle myosin IIA (NMHC IIA), a critical component of the actin cytoskeleton that plays essential roles in various cellular processes. Structurally, the MYH9 gene spans over 106 kilobases and includes 41 exons that translate into a protein of 1,960 amino acids. This protein is a part of a hexameric complex, which includes two heavy chains, two regulatory light chains, and two essential light chains. The NMHC IIA protein interacts with actin filaments and is involved in cellular activities such as cell migration, adhesion, division, and maintenance of cell shape. Functionally, mutations in MYH9 can result in a spectrum of autosomal dominant disorders collectively known as MYH9-related diseases (MYH9-RD), which include conditions such as May-Hegglin anomaly, Fechtner syndrome, and Epstein syndrome. These disorders are primarily characterized by macrothrombocytopenia (abnormally large platelets) and may lead to other complications such as hearing loss, renal failure, and cataracts later in life. MYH9 is also crucial in hematopoiesis, where its proper function is necessary for the survival and maintenance of hematopoietic stem and progenitor cells (HSPCs). Loss of MYH9 function disrupts normal hematopoiesis, leading to severe blood cell deficiencies and bone marrow failure.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

Product References

Molecular determinants of peri-apical targeting of inositol 1,4,5-trisphosphate receptor type 3 in cholangiocytes.

Hepatology communications

Rodrigues MA,Gomes DA,Fiorotto R,Guerra MT,Weerachayaphorn J,Bo T,Sessa WC,Strazzabosco M,Nathanson MH

PA5-17025 was used in Western Blot to investigate the mechanism by which ITPR3 localizes to the apical region to regulate secretion.

Sat Oct 01 00:00:00 UTC 2022

Molecular determinants of peri-apical targeting of inositol 1,4,5-trisphosphate receptor type 3 in cholangiocytes.

Hepatology communications

Rodrigues MA,Gomes DA,Fiorotto R,Guerra MT,Weerachayaphorn J,Bo T,Sessa WC,Strazzabosco M,Nathanson MH

PA5-17025 was used in Western Blot to investigate the mechanism by which ITPR3 localizes to the apical region to regulate secretion.

Sat Oct 01 00:00:00 UTC 2022

Molecular determinants of peri-apical targeting of inositol 1,4,5-trisphosphate receptor type 3 in cholangiocytes.

Hepatology communications

Rodrigues MA,Gomes DA,Fiorotto R,Guerra MT,Weerachayaphorn J,Bo T,Sessa WC,Strazzabosco M,Nathanson MH

PA5-17025 was used in Western Blot to investigate the mechanism by which ITPR3 localizes to the apical region to regulate secretion.

Sat Oct 01 00:00:00 UTC 2022

A resilient formin-derived cortical actin meshwork in the rear drives actomyosin-based motility in 2D confinement.

Nature communications

Ramalingam N,Franke C,Jaschinski E,Winterhoff M,Lu Y,Brühmann S,Junemann A,Meier H,Noegel AA,Weber I,Zhao H,Merkel R,Schleicher M,Faix J

PA5-17025 was used in immunocytochemistry to investigate how the cortical actin meshwork in the rear develops

Tue Sep 29 00:00:00 UTC 2015

A resilient formin-derived cortical actin meshwork in the rear drives actomyosin-based motility in 2D confinement.

Nature communications

Ramalingam N,Franke C,Jaschinski E,Winterhoff M,Lu Y,Brühmann S,Junemann A,Meier H,Noegel AA,Weber I,Zhao H,Merkel R,Schleicher M,Faix J

PA5-17025 was used in immunocytochemistry to investigate how the cortical actin meshwork in the rear develops

Tue Sep 29 00:00:00 UTC 2015

生物信息学

蛋白别名: Cellular myosin heavy chain, type A; flectin; MGC104539; Myosin heavy chain 9; myosin heavy chain IX; Myosin heavy chain, non-muscle IIa; myosin IIA; Myosin-9; myosin-9-like; myosin9; NMHC II-A; NMHC-II A; NMMHC II-a; NMMHC IIA; NMMHC-A; non-muscle myosin heavy chain 9; Non-muscle myosin heavy chain A; Non-muscle myosin heavy chain IIa; non-muscle myosin heavy polypeptide 9; nonmuscle myosin heavy chain II-A

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基因别名: BDPLT6; DFNA17; EPSTS; Fltn; FTNS; MHA; MYH9; Myhn-1; Myhn1; NMHC-II-A; NMHCIIA; NMMHC-A; NMMHC-IIA; NMMHCA; TU72.6

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UniProt ID: (Human) P35579, (Mouse) Q8VDD5

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Entrez Gene ID: (Rat) 100911597, (Human) 4627, (Mouse) 17886

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功能