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Invitrogen
Metallothionein Polyclonal Antibody
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Metallothionein Antibody (PA5-77772) in WB
Western blot analysis of Metallothionein in pseudomonas aeruginosa purified protein using 25 µg of protein. Sample was prepared with 1% milk, 2% BSA, incubated with Metallothionein polyclonal antibody (Product # PA5-77772) using a dilution of 1:1000 (16 hours at 4°C) and followed by a Mouse Anti-Rabbit secondary antibody at a dilution of 1:1000 (1 hour at RT). Samples are arranged as follows: Lane 1: molecular weight ladder, Lane 2: Blank, Lane 3: Pseudomonas aeruginosa purified protein with GST tag.
产品信息
PA5-77772
产品规格
种属反应
Bacteria
宿主/亚型
Rabbit
/ IgG
分类
Polyclonal
类型
Antibody
抗原
Synthetic peptide from the mid-protein of Pseudomonas aeruginosa Metallothionein
偶联物
形式
Liquid
浓度
1 mg/mL
纯化类型
Antigen affinity chromatography
保存液
PBS, pH 7.4, with 50% glycerol
内含物
0.09% sodium azide
保存条件
-20°C
运输条件
Ambient (domestic); Wet ice (international)
RRID
产品详细信息
A 1:1,000 dilution of PA5-77772 was sufficient for detection of Metallothionein in 15 µg of pseudomonas aeruginosa purified Metallothionein (pmtA-GST) protein by ECL immunoblot analysis using goat anti-rabbit IgG:HRP as the secondary antibody.|Detects approximately 36 kDa.
靶标信息
Metallothioneins (MTs) are a family of low molecular weight, heavy metal-binding proteins characterized by a high cysteine content and lack of aromatic amino acids. MTs bind 7 to 12 heavy metal atoms per molecule of protein. They are ubiquitous in the animal and plant kingdoms and are also found in prokaryotes. In mammals, the cysteine residues are absolutely conserved and serve to coordinate heavy metal atoms such as zinc, cadmium, and copper via mercaptide linkages.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
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生物信息学
蛋白别名: CES 1; CES1; Metallothionein; Metallothionein 2; Metallothionein 2A; Metallothionein IA; Metallothionein II; Metallothionein2; MGC32848; MT 1A; MT 2; MT 2A; MT IA; MT II; MT2; MT2A; Probable pmtA
