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Invitrogen
Mouse IgG2b kappa Isotype Control (eBMG2b), eFluor™ 506, eBioscience™
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Mouse IgG2b kappa Isotype Control (69-4732-81) in Flow
C57BL/6 mouse splenocytes were stained with CD19 Monoclonal Antibody, PE-Cyanine7 (Product # 25-0199-82) and staining buffer (autofluorescence) (left) or 0.125 µg of Mouse IgG2b Isotype control Monoclonal Antibody, eFluor 506 (right). Cells in the lymphocyte gate were used for analysis.
产品信息
69-4732-81
应用
建议稀释比
流式细胞分析 (Flow)
Assay-Dependent
对照试剂 (Ctrl)
Assay-Dependent
产品规格
宿主/亚型
Mouse
/ IgG2b, kappa
分类
Monoclonal
类型
Isotype Control
克隆号
eBMG2b
偶联物
激发/发射光谱
419/508 nm
查看光谱
形式
Liquid
浓度
0.2 mg/mL
纯化类型
Affinity chromatography
保存液
PBS, pH 7.2
内含物
0.09% sodium azide
保存条件
4° C, store in dark, DO NOT FREEZE!
运输条件
Ambient (domestic); Wet ice (international)
RRID
AB_2811922
产品详细信息
Description: The monoclonal mouse IgG2b is useful as an isotype control immunoglobulin.
Applications Reported: This eBMG2b antibody has been reported for use in flow cytometric analysis.
Applications Tested: This eBMG2b antibody has been tested by flow cytometric analysis of normal human peripheral blood cells. This should be used at the same concentration as the experimental antibody.
eFluor 506 can be excited with the violet laser line (405 nm) and emits at 506 nm. We recommend using a 510/20 band pass filter, or equivalent. Please make sure that your instrument is capable of detecting this fluorochrome.
Excitation: 405 nm; Emission: 506 nm; Laser: Violet Laser
靶标信息
Mouse IgG2b is a subtype of immunoglobulin G antibody that is produced by B cells in response to an antigen. It is the third most abundant IgG subtype in the serum and plays a crucial role in the adaptive immune response. Mouse IgG2b is composed of two heavy chains and two light chains, and it is involved in opsonization, complement activation, and antibody-dependent cell-mediated cytotoxicity. It is also involved in the regulation of immune responses, including the activation of T cells and the inhibition of B cell activation. Mouse IgG2b is widely used in research and diagnostic applications, including ELISA, Western blotting, and immunohistochemistry. It is also used in therapeutic applications, such as immunotherapy and vaccine development.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
生物信息学
蛋白别名: IgG2; Immunoglobulin G; Immunoglobulin G2; ImmunoglobulinG; ImmunoglobulinG2
