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Invitrogen
Mouse IgM Isotype Control, FITC
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产品信息
PA5-33229
应用
建议稀释比
流式细胞分析 (Flow)
Assay-dependent
对照试剂 (Ctrl)
Assay-Dependent
产品规格
宿主/亚型
Mouse
/ IgM
分类
Control
类型
Isotype Control
偶联物
激发/发射光谱
498/517 nm
查看光谱
形式
Liquid
浓度
0.1 mg/mL
规格
100 µg
保存条件
4°C, store in dark, DO NOT FREEZE!
运输条件
Wet ice
RRID
产品详细信息
PA5-33229 can be used as a negative control in flow cytometry when using mouse monoclonal antibodies in human cell surface antigens of isotype IgM. This enables an estimation of non-specific binding of mouse monoclonal antibodies to cell surface components. This antibody recognizes a rat cell surface marker and therefore cannot be used as a negative control in the species.
For FACS analysis, use 10 µL of the suggested working dilution to label 1x10^6 cells in 100 µL.
靶标信息
Mouse IgM is an immunoglobulin M antibody that is produced by B cells in response to an antigen. It is the first antibody produced during an immune response and is the largest immunoglobulin in the serum. Mouse IgM is composed of five heavy chains and five light chains, and it is involved in complement activation and agglutination of antigens. It plays a crucial role in the innate immune response and is important for neutralizing pathogens before the adaptive immune response is fully activated. Mouse IgM is widely used in research and diagnostic applications, including ELISA, Western blotting, and immunohistochemistry.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
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