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Mouse IgM Isotype Control (14-4752-82) in ICC/IF
Immunocytochemistry of fixed PC-12 cells stained with 20 µg/mL of Mouse IgM Isotype Control Purified (left) or 20 µg/mL of Anti-Polysialylated Neural Cell Adhesion Molecule (PSA-NCAM) Purified (right), followed by Anti-mouse IgM Biotin and Streptavidin FITC.Nuclei are stained with DAPI.
产品信息
14-4752-82
应用
建议稀释比
免疫组化（石蜡） (IHC (P))
Assay-Dependent
免疫细胞化学 (ICC/IF)
Assay-Dependent
流式细胞分析 (Flow)
Assay-Dependent
对照试剂 (Ctrl)
Assay-Dependent
产品规格
宿主/亚型
Mouse
/ IgM
分类
Monoclonal
类型
Isotype Control
克隆号
11E10
偶联物
形式
Liquid
浓度
0.5 mg/mL
纯化类型
Affinity chromatography
保存液
PBS, pH 7.2
内含物
0.09% sodium azide
保存条件
4° C
运输条件
Ambient (domestic); Wet ice (international)
RRID
AB_470123
产品详细信息
Description: The mouse IgM is useful as an isotype control immunoglobulin. The immunoglobulin was generated by immunization with lipopolysaccharide (LPS). The immunoglobulin is reactive with LPS.
Applications Reported: This mouse IgM isotype control has been reported for use in immunocytochemistry, immunohistochemistry, and flow cytometric analysis.
Applications Tested: Mouse IgM Isotype Control Purified has been tested by flow cytometric analysis. Use at the same concentration as the experimental antibody.
Purity: Greater than 90%, as determined by SDS-PAGE.
Aggregation: Less than 10%, as determined by HPLC.
Filtration: 0.2 µm post-manufacturing filtered.
靶标信息
Mouse IgM is an immunoglobulin M antibody that is produced by B cells in response to an antigen. It is the first antibody produced during an immune response and is the largest immunoglobulin in the serum. Mouse IgM is composed of five heavy chains and five light chains, and it is involved in complement activation and agglutination of antigens. It plays a crucial role in the innate immune response and is important for neutralizing pathogens before the adaptive immune response is fully activated. Mouse IgM is widely used in research and diagnostic applications, including ELISA, Western blotting, and immunohistochemistry.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
