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Human IgM Secondary Antibody (MII0401) in WB
Western blot was performed using Mouse anti-Human IgM Secondary Antibody (Product # MII0401) and an ~80 kDa band corresponding to Human IgM Heavy Chain was observed in Raji, Ramos and Daudi but not in IM-9, ARH-77, MOLT-4 and Jurkat8203. Whole cell extracts (30 µg) of IM-9 (Lane 1), ARH-77 (Lane 2), Raji (Lane 3), Ramos (Lane 4), Daudi (Lane 5), MOLT-4 (Lane 6) and Jurkat (Lane 7) were electrophoresed using NuPAGE™ 4-12% Bis-Tris Protein Gel (Product # NP0322BOX). Resolved proteins were then transferred onto a nitrocellulose membrane (Prod... View More
产品信息
MII0401
应用
建议稀释比
免疫印迹 (WB)
0.5-2 µg/mL
流式细胞分析 (Flow)
1 µg/mL
酶联免疫吸附实验 (ELISA)
1 µg/mL
免疫沉淀 (IP)
Assay-dependent
放射免疫测定 (RIA)
Assay-dependent
产品规格
种属反应
Human
宿主/亚型
Mouse
/ IgG1
分类
Monoclonal
类型
Secondary Antibody
克隆号
P5E2
抗原
Purified Human Serum IgM
偶联物
形式
Liquid
浓度
5 mg/mL
纯化类型
Protein A
保存液
PBS, pH 7.4
内含物
0.09% sodium azide
保存条件
Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C
运输条件
Ambient (domestic); Wet ice (international)
RRID
AB_11153935
靶标
IgM
抗体形式
Whole Antibody
产品详细信息
By ELISA and WB, MII0401 does not react with Human IgM or Human IgA.
MII0401 is also sold as a smaller package size (Product # MA5-14729).
MII0401 was formerly sold as a Seradyn product.
靶标信息
Anti-Human secondary antibodies are affinity-purified antibodies with well-characterized specificity for human immunoglobulins and are useful in the detection, sorting or purification of its specified target. Secondary antibodies offer increased versatility enabling users to use many detection systems (e.g. HRP, AP, fluorescence). They can also provide greater sensitivity through signal amplification as multiple secondary antibodies can bind to a single primary antibody. Most commonly, secondary antibodies are generated by immunizing the host animal with a pooled population of immunoglobulins from the target species and can be further purified and modified (i.e. immunoaffinity chromatography, antibody fragmentation, label conjugation, etc.) to generate highly specific reagents.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
