NeoBiotechnologies

Myogenin/Myf-4 (Skeletal Muscle Marker) (Transcription Factor) Monoclonal Antibody (PCRP-MYOG-1C5)

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问题与答案

问题与答案

抗体检测数据 (4)

Myogenin/Myf-4 (Skeletal Muscle Marker) (Transcription Factor) Antibody in Immunocytochemistry (ICC/IF)

Myogenin/Myf-4 (Skeletal Muscle Marker) (Transcription Factor) Antibody in Immunocytochemistry (ICC/IF)

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Myogenin/Myf-4 (Skeletal Muscle Marker) (Transcription Factor) Antibody (4656-MSM17-P0) in ICC/IF

Immunofluorescence analysis of PFA-fixed HeLa cells stained using Myogenin Mouse Monoclonal Antibody (PCRP-MYOG-1C5) followed by goat anti-mouse IgG-CF488 (green). CF640A phalloidin (red).

Myogenin/Myf-4 (Skeletal Muscle Marker) (Transcription Factor) Antibody in Immunocytochemistry (ICC/IF)

Myogenin/Myf-4 (Skeletal Muscle Marker) (Transcription Factor) Antibody in Flow Cytometry (Flow)

Myogenin/Myf-4 (Skeletal Muscle Marker) (Transcription Factor) Antibody in Peptide array (ARRAY)

Myogenin/Myf-4 (Skeletal Muscle Marker) (Transcription Factor) Antibody in SDS-PAGE (SDS-PAGE)

Product Image Gallery

Myogenin/Myf-4 (Skeletal Muscle Marker) (Transcription Factor) Antibody in Immunocytochemistry (ICC/IF) — Immunofluorescence analysis of PFA-fixed HeLa cells stained using Myogenin Mouse Monoclonal Antibody (PCRP-MYOG-1C5) followed by goat anti-mouse IgG-CF488 (green). CF640A phalloidin (red).

Myogenin/Myf-4 (Skeletal Muscle Marker) (Transcription Factor) Antibody in Flow Cytometry (Flow) — Flow cytometric analysis of PFA-fixed HeLa cells. Myogenin Mouse Monoclonal Antibody (PCRP-MYOG-1C5) followed by goat anti-mouse IgG-CF488 (blue); isotype control (red).

Myogenin/Myf-4 (Skeletal Muscle Marker) (Transcription Factor) Antibody in Peptide array (ARRAY) — Analysis of Protein Array containing more than 19,000 full-length human proteins using Myogenin Mouse Monoclonal Antibody (PCRP-MYOG-1C5). Z- and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody (MAb) (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProtTM array. Z-scores are described in units of standard deviations (SDs) above the mean value of all signals generated on that array. If targets on HuProtTM are arranged in descending order of the Z-score, the S-score is the diFerence (also in units of SDs) between the Z-score. S-score therefore represents the relative target specificity of a MAb to its intended target. A MAb is considered to specific to its intended target, if the MAb has an S-score of at least 2.5. For example, if a MAb binds to protein X with a Z-score of 43 and to protein Y with a Z-score of 14, then the S-score for the binding of that MAb to protein X is equal to 29.

Myogenin/Myf-4 (Skeletal Muscle Marker) (Transcription Factor) Antibody in SDS-PAGE (SDS-PAGE) — SDS-PAGE analysis Purified Myogenin Mouse Monoclonal Antibody (PCRP-MYOG-1C5). Confirmation of Integrity and Purity of Antibody.

产品信息

4656-MSM17-P0

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1-2 µg/mL

-

免疫细胞化学 (ICC/IF)

1-2 µg/mL

-

流式细胞分析 (Flow)

1-2 µg/10^6 cells

-

酶联免疫吸附实验 (ELISA)

Assay-dependent

-

免疫沉淀 (IP)

1-2 µg/100-500 µg total protein

-

肽阵列 (Array)

Assay-dependent

-

产品规格

种属反应

Human, Mouse, Rat

宿主/亚型

Mouse / IgG2b, kappa

分类

Monoclonal

类型

Antibody

克隆号

PCRP-MYOG-1C5

抗原

Human myogenin recombinant protein

偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

200 µg/mL

纯化类型

Protein A/G

保存液

PBS, pH 7.4, with 0.05% BSA

内含物

0.05% sodium azide

保存条件

4°C, do not freeze

运输条件

Ambient (domestic); Wet ice (international)

靶标信息

Myogenin is a muscle-specific transcription factor that can induce myogenesis in a variety of cell types in tissue culture. It is a member of a large family of proteins related by sequence homology, the helix-loop-helix proteins. It is essential for the development of functional skeletal muscle.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

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引用该产品

生物信息学

蛋白别名: bHLHc3; Class C basic helix-loop-helix protein 3; Myf-4; MYOD1-related protein; Myogenic factor 4; Myogenic factor-4; Myogenin

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基因别名: BHLHC3; myf-4; MYF4; myo; MYOG

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UniProt ID: (Human) P15173, (Mouse) P12979, (Rat) P20428

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Entrez Gene ID: (Human) 4656, (Mouse) 17928, (Rat) 29148

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RNA polymerase II regulatory region sequence-specific DNA binding RNA polymerase II core promoter proximal region sequence-specific DNA binding core promoter binding transcriptional activator activity, RNA polymerase II core promoter proximal region sequence-specific binding transcriptional activator activity, RNA polymerase II transcription regulatory region sequence-specific binding transcription factor activity, sequence-specific DNA binding transcription factor activity, RNA polymerase II distal enhancer sequence-specific binding protein binding chromatin DNA binding sequence-specific DNA binding protein heterodimerization activity E-box binding core promoter proximal region sequence-specific DNA binding DNA binding protein dimerization activity molecular_function basic helix-loop-helix transcription factor

ossification cell cycle skeletal muscle tissue development negative regulation of cell proliferation response to gravity positive regulation of myotube differentiation skeletal muscle atrophy positive regulation of muscle atrophy regulation of skeletal muscle satellite cell proliferation response to muscle activity involved in regulation of muscle adaptation response to electrical stimulus involved in regulation of muscle adaptation striated muscle atrophy response to denervation involved in regulation of muscle adaptation skeletal muscle cell differentiation muscle cell fate commitment mRNA transcription from RNA polymerase II promoter skeletal muscle tissue regeneration myoblast differentiation positive regulation of myoblast differentiation negative regulation of glycolytic process positive regulation of transcription from RNA polymerase II promoter skeletal muscle fiber development positive regulation of skeletal muscle fiber development positive regulation of muscle cell differentiation positive regulation of cell cycle arrest cellular response to magnetism cellular response to lithium ion cellular response to retinoic acid cellular response to growth factor stimulus cellular response to estradiol stimulus regulation of myoblast fusion positive regulation of myoblast fusion positive regulation of oxidative phosphorylation transcription, DNA-templated regulation of transcription, DNA-templated regulation of transcription from RNA polymerase II promoter transcription from RNA polymerase II promoter multicellular organism development muscle organ development myotube differentiation cell differentiation histone H3 acetylation histone H4 acetylation positive regulation of transcription, DNA-templated response to muscle activity