Invitrogen

NDUFA4 Polyclonal Antibody

Product Image Gallery

NDUFA4 Antibody in Western Blot (WB) — Fig. 4. C15ORF48 induces NDUFA4 protein degradation in stimulated macrophages. ( A and B ) Human MDMs transfected with control or miR-147b locked nucleic acid (LNA) antisense miRNA antagonist (25 nM), or nontransfected. (A) Cells unstimulated or treated with LPS (10 ng/ml) for 12 hours starting 1 day after transfection. NDUFA4 mRNA detected by RT-qPCR ( n = 4; means +- SEM). (B) Cells unstimulated or treated with LPS (10 ng/ml) for 24 hours starting 1 day after transfection. NDUFA4 protein detected by Western blotting. Representative blot of n = 3. Graph n = 3; means +- SEM. ( C ) CHX chase and Western blotting to determine NDUFA4 protein decay in human MDMs unstimulated [vehicle (Veh)] or treated with LPS (10 ng/ml) for the indicated times with or without addition of CHX (5 mug/ml). Samples collected at time points as indicated by arrows in experimental time line (top). Representative blot (left) and quantification by densitometry normalized to alpha-tubulin, reported relative to 0 hours CHX (right) ( n = 3; means +- SD). ( D ) C c O subunits detected by Western blotting in human MDMs unstimulated or treated with LPS (10 ng/ml) for 24 hours. Representative blot of n = 3. ( E ) Select subunits of ETC complexes detected by Western blotting in human MDMs unstimulated or treated with LPS (10 ng/ml) for 24 hours. Representative blot of n = 3. ( F and G ) Human MDMs transfected with control or C15orf48 small interfering RNA (siRNA) (25 nM), or nontransfected. (F) Cells unstimulate

产品信息

PA5-50068

应用	建议稀释比	已发表文章
免疫印迹 (WB)	1:500-1:3,000	查看1篇已发表文章 1篇已发表文章

产品规格
种属反应	Human, Mouse
已发表种属	Not Applicable
宿主/亚型	Rabbit / IgG
分类	Polyclonal
类型	Antibody
抗原	Synthesized peptide derived from internal of human NDUFA4. 查看免疫原
偶联物	Unconjugated
形式	Liquid
浓度	1 mg/mL
纯化类型	Antigen affinity chromatography
保存液	PBS, pH 7.4, with 50% glycerol
内含物	0.02% sodium azide
保存条件	-20°C
RRID	AB_2635521

产品详细信息

The antibody detects endogenous levels of total NDUFA4 protein.

靶标信息

Cytochrome c oxidase (COX, complex IV) is the terminal component of the mitochondrial respiratory chain that catalyzes the reduction of oxygen to water. Required for complex IV maintenance. {ECO:0000269; PubMed:22902835}.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

Product References

Inflammation causes remodeling of mitochondrial cytochrome c oxidase mediated by the bifunctional gene C15orf48.

Science advances

Clayton SA,Daley KK,MacDonald L,Fernandez-Vizarra E,Bottegoni G,O'Neil JD,Major T,Griffin D,Zhuang Q,Adewoye AB,Woolcock K,Jones SW,Goodyear C,Elmesmari A,Filer A,Tennant DA,Alivernini S,Buckley CD,Pitceathly RDS,Kurowska-Stolarska M,Clark AR

Published figure using NDUFA4 polyclonal antibody (Product # PA5-50068) in Western Blot

Fri Dec 10 00:00:00 EST 2021

生物信息学

蛋白别名: CI-MLRQ; Complex I 9kDa subunit; Complex I-MLRQ; Cytochrome c oxidase subunit NDUFA4; EC 1.6.5.3; EC 1.6.99.3; NADH dehydrogenase (ubiquinone) 1 alpha subcomplex, 4 (9 kDa, MLRQ); NADH dehydrogenase (ubiquinone) 1 alpha subcomplex, 4, 9kDa; NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 4; NADH-ubiquinone oxidoreductase MLRQ subunit

基因别名: CI-9k; CI-MLRQ; MLRQ; NDUFA4

UniProt ID: (Human) O00483, (Mouse) Q62425

Entrez Gene ID: (Human) 4697, (Mouse) 17992

分子生物学功能: oxidoreductase