Invitrogen

NDUFAF2 Polyclonal Antibody

高级验证

This Antibody was verified by Cell treatment to ensure that the antibody binds to the antigen stated.

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说明书

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NDUFAF2 Antibody in Immunocytochemistry (ICC/IF) — Immunofluorescence analysis of NADH dehydrogenase [ubiquinone] 1 alpha subcomplex assembly factor 2 was performed using 70% confluent log phase Hep G2 cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 2% BSA for 45 minutes at room temperature. The cells were labeled with NDUFAF2 Polyclonal Antibody (Product # PA5-50452) at 1:100 in 0.1% BSA, incubated at 4 degree celsius overnight and then labeled with Donkey anti-Rabbit IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor Plus 488 (Product # A32790), (1:2000), for 45 minutes at room temperature (Panel a: Green). Nuclei (Panel b:Blue) were stained with Hoechst 33342 (Product # H1399). F-actin (Panel c: Red) was stained with Rhodamine Phalloidin (Product # R415, 1:300). Panel d represents the merged image showing predominantly Mitochondrial localization. Panel e represents control cells with no primary antibody to assess background. The images were captured at 40X magnification in CellInsight CX7 LZR High-Content Screening (HCS) Platform (Product # CX7C1115LZR).

NDUFAF2 Antibody in Western Blot (WB) — Western blot was performed using Anti-NDUFAF2 Polyclonal Antibody (Product # PA5-50452) and a 20 kDa band corresponding to NADH dehydrogenase [ubiquinone] 1 alpha subcomplex assembly factor 2 was observed. Whole cell extracts (30 µg lysate) of Raji (Lane 1), PC-3 (Lane 2), HEK-293 (Lane 3), SK-BR-3 (Lane 4), HeLa (Lane 5), T98G (Lane 6), MCF7 (Lane 7) and tissue extracts (30 µg lysate) of Mouse Heart (Lane 8), Rat Heart (Lane 9) were electrophoresed using NuPAGE™ 12% Bis-Tris Protein Gel (Product # NP0342BOX). Resolved proteins were then transferred onto a nitrocellulose membrane (Product # IB23001) by iBlot® 2 Dry Blotting System (Product # IB21001). The blot was probed with the primary antibody (1:1000) and detected by chemiluminescence with Goat anti-Rabbit IgG (H+L) Superclonal™ Recombinant Secondary Antibody, HRP (Product # A27036, 1:4000) using the iBright FL 1000 (Product # A32752). Chemiluminescent detection was performed using Novex® ECL Chemiluminescent Substrate Reagent Kit (Product # WP20005).

NDUFAF2 Antibody — Altered expression of proteins upon cell treatment demonstrates antibody specificity. Western blot analysis (Fig a) using NDUFAF2 Polyclonal Antibody (Product # PA5-50452), shows upregulation of NDUFAF2 expression in Hep G2 cells treated with IL1 beta and IL6 (doi: 10.1186/1471-2121-10-23). Densitometric analysis of this western blot is shown in histogram (Fig. b). {TM}

产品信息

PA5-50452

应用	建议稀释比	已发表文章
免疫印迹 (WB)	1:500-1:2,000	-
免疫细胞化学 (ICC/IF)	1:100	-

产品规格
种属反应	Human
宿主/亚型	Rabbit / IgG
分类	Polyclonal
类型	Antibody
抗原	Fusion protein corresponding to a region derived from internal residues of human NADH dehydrogenase (ubiquinone) complex I, assembly factor 2
偶联物	Unconjugated
形式	Liquid
浓度	2.6 mg/mL
纯化类型	Antigen affinity chromatography
保存液	PBS, pH 7.4, with 40% glycerol
内含物	0.05% sodium azide
保存条件	-20°C
RRID	AB_2635905

产品详细信息

The antibody detects endogenous levels of total NDUFAF2 protein.

靶标信息

Mimitin, a small mitochondrial protein, whose transcription is directly stimulated by c-Myc, is highly expressed in 80% of esophageal squamous cell carcinomas (ESCC). Suppression of Mimitin expression by RNA interference had no effect in cancerous cell lines such as human cervical carcinoma or hepatocarcinoma cell lines, but caused a decrease in cell proliferation in human glioblastoma, embryonic lung fibroblastic cells, and ESCC, suggesting Mimitin may play a special role in these types of cells. Mimitin expression is also regulated by MAPK kinases and IL-1, but not through the NF-kappa-B-related pathway. It will interact with the microtubular protein MAP1S and can affect the activities of caspase-3 and -7 in cells stimulated to develop apoptosis. Other experiments suggest that Mimitin also acts as a molecular chaperone for the assembly of the mitochondrial complex I.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

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引用该产品

生物信息学

蛋白别名: B17.2-like; B17.2L; B172-like; B172L; Mimitin; MMTN; Myc-induced mitochondrial protein; NADH dehydrogenase (ubiquinone) 1 alpha subcomplex, assembly factor 2; NADH dehydrogenase (ubiquinone) complex I, assembly factor 2; NADH dehydrogenase 1 alpha subcomplex assembly factor 2; NADH dehydrogenase [ubiquinone] 1 alpha subcomplex assembly factor 2; NDUFA12-like protein

基因别名: B17.2L; mimitin; MMTN; NDUFA12L; NDUFAF2

UniProt ID: (Human) Q8N183

Entrez Gene ID: (Human) 91942

分子生物学功能: chaperone

功能

respiratory electron transport chain mitochondrial respiratory chain complex I assembly cellular respiration negative regulation of insulin secretion involved in cellular response to glucose stimulus reactive oxygen species metabolic process

参与通路

NADH dehydrogenase (ubiquinone) activity electron carrier activity

NDUFAF2 Polyclonal Antibody

产品信息

PA5-50452

免疫印迹 (WB)

免疫细胞化学 (ICC/IF)

种属反应

宿主/亚型

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类型

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偶联物

形式

浓度

纯化类型

保存液

内含物

保存条件

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产品详细信息

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生物信息学

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