Invitrogen

NEDL2 Polyclonal Antibody

查看其他6个NEDL2抗体

问题与答案

问题与答案

问题与答案

Additional Information:

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抗体检测数据 (4)

NEDL2 Antibody in Immunocytochemistry (ICC/IF)

NEDL2 Antibody in Immunocytochemistry (ICC/IF)

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NEDL2 Antibody (PA5-113497) in ICC/IF

Immunocytochemichal analysis of NEDL2 in A549 cells using a Polyclonal antibody (Product # PA5-113497) at a dilution of 1:166. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated Goat Anti-Rabbit IgG(H+L). Cells were also counter-stained with DAPI.

NEDL2 Antibody in Immunocytochemistry (ICC/IF)

NEDL2 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

NEDL2 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

NEDL2 Antibody in Western Blot (WB)

Please note: We are reviewing Western blot images included in the antibody testing data in our catalog, including those provided by third parties. Unless expressly labeled or annotated as “raw-unedited”, Western blot images included in the antibody testing data in our catalog may have been edited, optimized or otherwise adjusted for presentation.

Product Image Gallery

NEDL2 Antibody in Immunocytochemistry (ICC/IF) — Immunocytochemichal analysis of NEDL2 in A549 cells using a Polyclonal antibody (Product # PA5-113497) at a dilution of 1:166. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated Goat Anti-Rabbit IgG(H+L). Cells were also counter-stained with DAPI.

NEDL2 Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunocytochemical analysis of paraffin-embeded NEDL2 in human brain tissue using a NEDL2 Polyclonal antibody (Product # PA5-113497) at a dilution of 1:500.

NEDL2 Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunocytochemical analysis of paraffin-embeded NEDL2 in human glioma using a NEDL2 Polyclonal antibody (Product # PA5-113497) at a dilution of 1:500.

NEDL2 Antibody in Western Blot (WB) — Western Blot analysis of NEDL2 in multiple sample types using a NEDL2 polyclonal antibody (Product # PA5-113497) at a concentration of 3.5 µg/ml. A goat anti rabbit polyclonal secondary antibody was used at a dilution of 1:50,000. Sample types that were tested include: Hela whole cell lysate, HepG2 whole cell lysate, A549 whole cell lysate, 293T whole cell lysate .

Immunogen image

NEDL2 Polyclonal Antibody

Immunogen Image NEDL2 Polyclonal Antibody

产品信息

PA5-113497

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1:500-1:5,000

-

免疫组化（石蜡） (IHC (P))

1:500-1:1,000

-

免疫细胞化学 (ICC/IF)

1:50-1:200

-

酶联免疫吸附实验 (ELISA)

Assay-dependent

-

产品规格

种属反应

Human

宿主/亚型

Rabbit / IgG

分类

Polyclonal

类型

Antibody

抗原

Recombinant Human E3 ubiquitin-protein ligase HECW2 protein (495-641AA)

3D表位/免疫原

偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

1 mg/mL

100 µg

纯化类型

Protein G

保存液

PBS, pH 7.4, with 50% glycerol

内含物

0.03% ProClin 300

保存条件

-20°C or -80°C if preferred

Ambient (domestic); Wet ice (international)

靶标信息

E3 ubiquitin-protein ligase that mediates ubiquitination of TP73. Acts to stabilize TP73 and enhance activation of transcription by TP73.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

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Cite this product

生物信息学

蛋白别名: hect; HECT-type E3 ubiquitin transferase HECW2; NEDD4-like E3 ubiquitin-protein ligase 2; NEDD4-related E3 ubiquitin ligase NEDL2

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基因别名: NDHSAL; NEDL2

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UniProt ID: (Human) Q9P2P5

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Entrez Gene ID: (Human) 57520

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ubiquitin-protein transferase activity protein binding ubiquitin protein ligase activity

ubiquitin-dependent protein catabolic process protein ubiquitination regulation of mitotic metaphase/anaphase transition regulation of dendrite morphogenesis