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Invitrogen
NF1 Monoclonal Antibody (McNFn27b)
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NF1 Antibody (MA1-085) in ICC/IF
Immunofluorescence analysis of Neurofibromin was done on 70% confluent log phase SH-SY5Y cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 10 minutes, and blocked with 1% BSA for 1 hour at room temperature. The cells were labeled with Neurofibromin (McNFn27b) Mouse Monoclonal Antibody (Product # MA1-085) at 2 µg/mL in 0.1% BSA and incubated for 3 hours at room temperature and then labeled with Goat anti-Mouse IgG (H+L) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate (... View More
产品信息
MA1-085
产品规格
宿主/亚型
Mouse
/ IgG1
分类
Monoclonal
类型
Antibody
克隆号
McNFn27b
抗原
Synthetic peptide corresponding to the N-terminal residues 27-41 of human neurofibromin.
偶联物
形式
Liquid
浓度
1 mg/mL
保存条件
-20°C, Avoid Freeze/Thaw Cycles
运输条件
Ambient (domestic); Wet ice (international)
RRID
产品详细信息
MA1-085 detects neurofibromin in human, rat, and mouse cells.
MA1-085 has been successfully used in Western blot, and immunohistochemistry procedures. By Western blot, this antibody detects a ~240-260 kDa protein.
The MA1-085 immunogen is a synthetic peptide corresponding to the N-terminal residues 27-41 of human neurofibromin.
靶标信息
The protein encoded by this gene is a type I integral membrane protein localized in the intermediate region between the endoplasmic reticulum and the Golgi, presumably recycling between the two compartments. The protein is a mannose-specific lectin and is a member of a novel family of plant lectin homologs in the secretory pathway of animal cells. Mutations in the gene are associated with a coagulation defect. Using positional cloning, the gene was identified as the disease gene leading to combined factor V-factor VIII deficiency, a rare, autosomal recessive disorder in which both coagulation factors V and VIII are diminished.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
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