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Invitrogen
NFIX Polyclonal Antibody
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NFIX Antibody (PA5-31234) in ICC/IF
NFIX Polyclonal Antibody detects NFIX protein by immunofluorescent analysis. Sample: DIV9 rat hippocampal neuron cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: NFIX stained by NFIX Polyclonal Antibody (Product # PA5-31234) diluted at 1:500. Red: Tau, a Axon marker, stained by Tau antibody [GT287] diluted at 1:500. Blue: Fluoroshield with DAPI .
产品信息
PA5-31234
应用
建议稀释比
已发表文章
产品规格
已发表种属
Human
宿主/亚型
Rabbit
/ IgG
分类
Polyclonal
类型
Antibody
抗原
Recombinant fragment corresponding to a region within amino acids 116 and 361 of Human NFIX
偶联物
形式
Liquid
浓度
0.76 mg/mL
保存条件
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
运输条件
Wet ice
RRID
产品详细信息
Recommended positive controls: 293T.
Predicted reactivity: Mouse (99%), Xenopus laevis (85%).
Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
靶标信息
NF-1, also designated CTF, consists of a family of CCAAT box binding proteins that stimulate DNA replication and activate transcription. Analysis of human NF-1 messenger RNA has revealed two forms of the NF-1 protein arising from an alternate splicing of a single NF-1 gene. NF-1 binds its consensus DNA element as a homodimer via an amino-terminal DNA binding domain, and activates transcription through a putatively novel, proline-rich, carboxy terminal transactivation domain. The NF-1 protein has been shown to recognize and bind the adenovirus type 2 promoter and activate transcription of herpes simplex virus thymidine kinase genes. The NF-1 consensus element has been found in the upstream promoter region of myriad eukaryotic genes, including that of Ha-Ras, alpha-globin, HSP 70, GRP 78, Histone H1, myelin basic protein and in the Xenopus laevis vitellogenin gene promoter.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
