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Invitrogen
NFYC Polyclonal Antibody
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NFYC Antibody (PA5-117162) in WB
Western blot analysis of NFYC in rat thymus. Samples were incubated with NFYC Polyclonal antibody (Product # PA5-117162) using a dilution of 1:400, followed by HRP Goat Anti-Rabbit IgG (H+L) at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 180s.
产品信息
PA5-117162
产品规格
种属反应
Mouse
宿主/亚型
Rabbit
/ IgG
分类
Polyclonal
类型
Antibody
抗原
Recombinant protein (or fragment)
偶联物
形式
Liquid
浓度
0.8 mg/mL
纯化类型
Affinity chromatography
保存液
PBS, pH 7.3, with 50% glycerol
内含物
0.02% sodium azide
保存条件
-20°C, Avoid Freeze/Thaw Cycles
运输条件
Ambient (domestic); Wet ice (international)
RRID
产品详细信息
Positive Samples: Mouse kidney, Mouse heart
Immunogen sequence: MEEIRNLTVK DFRVQELPLA RIKKIMKLDE DVKMISAEAP VLFAKAAQIF ITELTLRAWI HTEDNKRRTL QRNDIAMAIT KFDQFDFLID IVPR
靶标信息
The CCAAT-binding factor NF-Y is a heteromeric transcription factor that specifically binds to CCAAT sequences in many eukaryotic genes. NF-Y is made up of three subunits, NF-YA, NF-YB and NF-YC. All three components are necessary for DNA binding. In each NF-Y subunit, the segment needed for formation of the NF-Y-DNA complex is conserved from yeast to human. These conserved segments are homologous to the histone-fold motif of eukaryotic histones. The DNA binding domains of the NF-YB and NF-YC subunits have been suggested to interact through a protein-protein histone-fold "handshake" motif in a manner analogous to the histone proteins, H2B and H2A, respectively.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
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生物信息学
蛋白别名: CAAT box DNA-binding protein subunit C; DKFZp667G242; FLJ45775; NF-YC; Nuclear transcription factor Y subunit C; Nuclear transcription factor Y subunit gamma
基因别名: Nfyc
UniProt ID: (Mouse) P70353
Entrez Gene ID: (Mouse) 18046
RNA polymerase II core promoter proximal region sequence-specific DNA binding
transcriptional activator activity, RNA polymerase II core promoter proximal region sequence-specific binding
DNA binding
transcription factor activity, sequence-specific DNA binding
transcription factor binding
sequence-specific DNA binding
transcription regulatory region DNA binding
protein heterodimerization activity
DNA-binding transcription factor
