Invitrogen

NOTCH3 Recombinant Rabbit Monoclonal Antibody (4H5L17)

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NOTCH3 Antibody (701975) in WB

Western blot analysis was performed on Whole cell extracts (30 µg lysate) of MCF-7 (Lane 1), U-87 MG (Lane 2), SH-SY5Y (Lane 3), NTERA-2 (Lane 4), MDA-MB-231 (Lane 5), T-47D (Lane 6), U-2-OS (Lane 7) and A-431 (Lane 8). The blots were probed with Anti-NOTCH3 Recombinant Rabbit Monoclonal Antibody (Product # 701975, 1-2 µg/mL) and detected by chemiluminescence using Goat anti-Rabbit IgG (Heavy Chain) Superclonal Secondary Antibody, HRP conjugate (Product # A27036, 0.4 µg/mL, 1:2500 dilution). A 110 kDa (cleaved fragment) band corresponding ... View More

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产品信息

701975

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1-2 µg/mL

产品规格

种属反应

Human

宿主/亚型

Rabbit / IgG

Expression System

Expi293

分类

Recombinant Monoclonal

类型

Antibody

克隆号

4H5L17

抗原

Peptides corresponding to human Notch3 (aa2308-2321, 1618-1636, 1803-1816)

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偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

0.5 mg/mL

纯化类型

Protein A

保存液

PBS, pH 7.4

内含物

0.09% sodium azide

保存条件

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

运输条件

Wet ice

AB_2665150

产品详细信息

This antibody is predicted to react with Monkey, Bovine, Rat, Cat

Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems. The expression systems are developed by cloning in the specific antibody DNA sequences from immunoreactive rabbits. Then, individual clones are screened to select the best candidates for production. The advantages of using recombinant rabbit monoclonal antibodies include: better specificity and sensitivity, lot-to-lot consistency, animal origin-free formulations, and broader immunoreactivity to diverse targets due to larger rabbit immune repertoire.

靶标信息

NOTCH3 is the third discovered human homologue of the Drosophilia melanogaster type I membrane protein notch. In Drosophilia, notch interaction (with its cell-bound ligands delta, serrate) establishes an intercellular signalling pathway that plays a key role in neural development. Homologues of the notch-ligands have also been identified in human. NOTCH3 functions as a receptor for membrane-bound ligands Jagged1, Jagged2, and Delta1 to regulate cell-fate determination. Mutations in NOTCH3 have been identified as the underlying cause of cerebral autosomal dominant arteriopathy with subcortical infarcts and leukoencephalopathy (CADASIL).

仅用于科研。不用于诊断过程。未经明确授权不得转售。