Invitrogen

NOVA1 Polyclonal Antibody

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NOVA1 Antibody in Immunocytochemistry (ICC/IF) — Immunofluorescence analysis of NOVA1 in A549 cell. Antigen retrieval was performed on the tissue using IHC enzyme antigen retrieval reagent and blocked with 10% goat serum. Samples were incubated with NOVA1 polyclonal antibody (Product # PA5-95571) at a 2 µg/mL dilution, followed by 488 conjugated goat anti-rabbit IgG using a 1:100 dilution.

NOVA1 Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry analysis of NOVA1 in paraffin-embedded human glioma tissue. Antigen retrieval was performed on the tissue using citrate buffer (pH 6, 20 min) and blocked with 10% goat serum. Samples were incubated with NOVA1 polyclonal antibody (Product # PA5-95571) at a 1 µg/mL dilution, followed by biotinylated goat anti-rabbit IgG (30 min, 37°C), and developed with Strepavidin-Biotin-Complex and DAB.

NOVA1 Antibody in Immunohistochemistry (Frozen) (IHC (F)) — Immunohistochemistry analysis of NOVA1 in paraffin-embedded human placenta tissues. Antigen retrieval was performed on the tissue using citrate buffer (pH 6, 20 min) and blocked with 10% goat serum. Samples were incubated with NOVA1 polyclonal antibody (Product # PA5-95571) at a 1 µg/mL dilution, followed by biotinylated goat anti-rabbit IgG (30 min, 37°C), and developed with Strepavidin-Biotin-Complex and DAB.

NOVA1 Antibody in Western Blot (WB) — Western blot analysis of NOVA1 in Lane 1: human U-87MG whole cell lysate, Lane 2: human Caco-2 whole cell lysate. Electrophoresis was performed with 5-20% SDS-PAGE gel (70V, Stacking gel; 90V Resolving gel, Time: 2-3 hours), transferred to a nitrocellulose membrane and blocked using 5% Non-fat Milk/TBS (1.5 hrs at room temperature). Samples were incubated with NOVA1 polyclonal antibody (Product # PA5-95571) using a 0.5 µg/mL dilution, followed by a goat anti-rabbit IgG-HRP at a dilution of 1:10,000, and developed with enhanced chemiluminescence (ECL).

产品细节

Applications

Tested Dilution

Publications

免疫印迹 (WB)

0.1-0.5 µg/mL

免疫组化（石蜡） (IHC (P))

0.5-1 µg/mL

免疫组化（冰冻） (IHC (F))

0.5-1 µg/mL

免疫细胞化学 (ICC/IF)

2 µg/mL

酶联免疫吸附实验 (ELISA)

0.1-0.5 µg/mL

产品规格

种属反应

Human, Mouse, Rat

宿主/亚型

Rabbit / IgG

分类

Polyclonal

类型

Antibody

抗原

E.coli-derived human Nova1 recombinant protein (Position: T416-V509).

偶联物

Unconjugated

形式

Lyophilized

浓度

500 µg/mL

纯化类型

Affinity chromatography

保存液

PBS with 4mg trehalose

内含物

0.05mg sodium azide

保存条件

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

RRID

AB_2807373

产品详细信息

Reconstitution information: Add 0.2 mL of distilled water will yield a concentration of 500 µg/mL.

靶标信息

This gene encodes a neuron-specific RNA-binding protein, a member of the Nova family of paraneoplastic disease antigens, that is recognized and inhibited by paraneoplastic antibodies. These antibodies are found in the sera of patients with paraneoplastic opsoclonus-ataxia, breast cancer, and small cell lung cancer. Alternatively spliced transcripts encoding distinct isoforms have been described.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

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生物信息学

蛋白别名: Neuro-oncological ventral antigen 1; Onconeural ventral antigen 1; Paraneoplastic Ri antigen; RNA-binding protein Nova-1; Ventral neuron-specific protein 1

基因别名: 9430099M15Rik; G630039L02; Nova-1; NOVA1

UniProt ID: (Human) P51513, (Mouse) Q9JKN6

Entrez Gene ID: (Human) 4857, (Mouse) 664883, (Rat) 298992

分子生物学功能: RNA metabolism protein